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Article: Identification and cloning of main immune anlage cDNA of infectious bursal disease variant strain virus

TitleIdentification and cloning of main immune anlage cDNA of infectious bursal disease variant strain virus
傳染性法氏囊病病毒變異株主要免疫原基因cDNA的克隆及鑒定
Authors
KeywordsInfectious bursal disease virus (IBDV)
Variant strain
Gene cloning
DNA sequence
Issue Date1997
Publisher中國農業大學出版社. The Journal's web site is located at http://www.cnki.com.cn/Journal/D-D5-ZSYZ.htm
Citation
中國獸醫雜誌, 1997, v. 23 n. 9, p. 3-5 How to Cite?
Chinese Journal of Veterinary Medicine, 1997, v. 23 n. 9, p. 3-5 How to Cite?
AbstractUsing Reverse Transcription Polymerase Chain Reaction (RT PCR) technique,the cDNA fragment with length of about 1350bp coding for immunodominant protein VP 2 of infectious bursal disease virus (IBDV) was amplified from Gz902,a variant strain isolated from Guangdong Province.The fragment digested with BgII and EcoRI were inserted into the BamHI/EcoRI sites of pBluescriptII KS vector,and the recombinant plasmid were transformed into E.coli strain XLI Blue.The positive recombinant colonies were identified by PCR and restriction endonuclease digestion.The nucleotide sequence of hypervariable region of VP 2 gene was analyzed using Autocycle DNA Sequencing kit.The result showed that the similarities of the DNA sequence of Gz902 strain with variant strain A,GLS and E are 98.9%,96.2%and 95.5% respectively.Comparing the deduced amino acid sequences,there is one amino acid changed in each of the two hydrophilc regions of Gz902 strain.At two positions (249 and 254),the amino acid residues of Gz902 strain are conserved in variant strain A,GLS and E,but differs from that of all the classical strains.These two amino acid residues can be regarded as the signature of variant strains of IBDV.
Persistent Identifierhttp://hdl.handle.net/10722/84636
ISSN

 

DC FieldValueLanguage
dc.contributor.authorCao, Yen_HK
dc.contributor.authorBi, YZen_HK
dc.contributor.authorLaw, MSen_HK
dc.contributor.authorYeung, WSen_HK
dc.contributor.authorLeung, FCCen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2010-09-06T08:55:21Z-
dc.date.available2010-09-06T08:55:21Z-
dc.date.issued1997en_HK
dc.identifier.citation中國獸醫雜誌, 1997, v. 23 n. 9, p. 3-5en_HK
dc.identifier.citationChinese Journal of Veterinary Medicine, 1997, v. 23 n. 9, p. 3-5-
dc.identifier.issn0529-6005-
dc.identifier.urihttp://hdl.handle.net/10722/84636-
dc.description.abstractUsing Reverse Transcription Polymerase Chain Reaction (RT PCR) technique,the cDNA fragment with length of about 1350bp coding for immunodominant protein VP 2 of infectious bursal disease virus (IBDV) was amplified from Gz902,a variant strain isolated from Guangdong Province.The fragment digested with BgII and EcoRI were inserted into the BamHI/EcoRI sites of pBluescriptII KS vector,and the recombinant plasmid were transformed into E.coli strain XLI Blue.The positive recombinant colonies were identified by PCR and restriction endonuclease digestion.The nucleotide sequence of hypervariable region of VP 2 gene was analyzed using Autocycle DNA Sequencing kit.The result showed that the similarities of the DNA sequence of Gz902 strain with variant strain A,GLS and E are 98.9%,96.2%and 95.5% respectively.Comparing the deduced amino acid sequences,there is one amino acid changed in each of the two hydrophilc regions of Gz902 strain.At two positions (249 and 254),the amino acid residues of Gz902 strain are conserved in variant strain A,GLS and E,but differs from that of all the classical strains.These two amino acid residues can be regarded as the signature of variant strains of IBDV.-
dc.languagechien_HK
dc.publisher中國農業大學出版社. The Journal's web site is located at http://www.cnki.com.cn/Journal/D-D5-ZSYZ.htm-
dc.relation.ispartof中國獸醫雜誌en_HK
dc.relation.ispartofChinese Journal of Veterinary Medicine-
dc.subjectInfectious bursal disease virus (IBDV)-
dc.subjectVariant strain-
dc.subjectGene cloning-
dc.subjectDNA sequence-
dc.titleIdentification and cloning of main immune anlage cDNA of infectious bursal disease variant strain virusen_HK
dc.title傳染性法氏囊病病毒變異株主要免疫原基因cDNA的克隆及鑒定-
dc.typeArticleen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.identifier.hkuros36112en_HK
dc.identifier.volume23-
dc.identifier.issue9-
dc.identifier.spage3-
dc.identifier.epage5-
dc.publisher.placeChina-

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