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Article: Use of two-dimensional gel electrophoresis to differentiate morphospecies of Alexandrium minutum, a paralytic shellfish poisoning toxin-producing dinoflagellate of harmful algal blooms

TitleUse of two-dimensional gel electrophoresis to differentiate morphospecies of Alexandrium minutum, a paralytic shellfish poisoning toxin-producing dinoflagellate of harmful algal blooms
Authors
KeywordsAlexandrium minutum
Harmful algal bloom species
Paralytic shellfish poisoning
Paralytic shellfish poisoning toxin
Two-dimensional gel electrophoresis
Issue Date2005
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2005, v. 5 n. 6, p. 1580-1593 How to Cite?
AbstractContamination of shellfish with paralytic shellfish poisoning toxins (PST) produced by toxic harmful algal blooms (HABs) have been negatively affecting the shellfish and aquaculture industries worldwide. Therefore, accurate and early identification of toxic phytoplankton species is crucial in HABs surveillance programs that allow fish-farmers to take appropriate preventive measures in shellfish harvesting and other aquaculture activities to overcome the negative impacts of HABs on human health. The identification of toxic dinoflagellates present in the water is currently a time-consuming operation since it requires skillful taxonomists and toxicologists equipped with optical and scanning electron microscopes as well as sophisticated equipment, for example, high-performance liquid chromotography-fluorescence detection. In this paper, a two-dimensional gel electrophoresis (2-DE)-based proteomic approach was applied to discriminate between toxic and nontoxic strains of Alexandrium minutum. Variation in morphological features between toxic and nontoxic strains was minimal and not significant. Also, variation in 2-DE protein patterns within either toxic or nontoxic strains was low, but pronounced differences were detected between toxic and nontoxic strains. The most notable differences between these strains were several abundant proteins with pIs ranging from 4.8 to 5.3 and apparent molecular masses between 17.5 and 21.5 kDa. Groups of proteins, namely NT1, NT2, NT3, and NT4, were consistently found in all nontoxic strains, while T1 and T2 were prominent in the toxic strains. These specific protein spots characteristic for toxic and nontoxic strains remained clearly distinguishable irrespective of the various growth conditions tested. Therefore, they have the potential to serve as "taxonomic markers" to distinguish toxic and nontoxic strains within A. minutum. Initial studies revealed that the expression pattern of T1 was tightly correlated to toxin biosynthesis in the examined alga and may be used to serve as a potential toxin indicator. © 2005 WILEY-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/84634
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, LLen_HK
dc.contributor.authorHodgkiss, IJen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorWan, JMFen_HK
dc.contributor.authorChou, HNen_HK
dc.contributor.authorLum, JHKen_HK
dc.contributor.authorLo, MGYen_HK
dc.contributor.authorMak, ASCen_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorLo, SCLen_HK
dc.date.accessioned2010-09-06T08:55:20Z-
dc.date.available2010-09-06T08:55:20Z-
dc.date.issued2005en_HK
dc.identifier.citationProteomics, 2005, v. 5 n. 6, p. 1580-1593en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84634-
dc.description.abstractContamination of shellfish with paralytic shellfish poisoning toxins (PST) produced by toxic harmful algal blooms (HABs) have been negatively affecting the shellfish and aquaculture industries worldwide. Therefore, accurate and early identification of toxic phytoplankton species is crucial in HABs surveillance programs that allow fish-farmers to take appropriate preventive measures in shellfish harvesting and other aquaculture activities to overcome the negative impacts of HABs on human health. The identification of toxic dinoflagellates present in the water is currently a time-consuming operation since it requires skillful taxonomists and toxicologists equipped with optical and scanning electron microscopes as well as sophisticated equipment, for example, high-performance liquid chromotography-fluorescence detection. In this paper, a two-dimensional gel electrophoresis (2-DE)-based proteomic approach was applied to discriminate between toxic and nontoxic strains of Alexandrium minutum. Variation in morphological features between toxic and nontoxic strains was minimal and not significant. Also, variation in 2-DE protein patterns within either toxic or nontoxic strains was low, but pronounced differences were detected between toxic and nontoxic strains. The most notable differences between these strains were several abundant proteins with pIs ranging from 4.8 to 5.3 and apparent molecular masses between 17.5 and 21.5 kDa. Groups of proteins, namely NT1, NT2, NT3, and NT4, were consistently found in all nontoxic strains, while T1 and T2 were prominent in the toxic strains. These specific protein spots characteristic for toxic and nontoxic strains remained clearly distinguishable irrespective of the various growth conditions tested. Therefore, they have the potential to serve as "taxonomic markers" to distinguish toxic and nontoxic strains within A. minutum. Initial studies revealed that the expression pattern of T1 was tightly correlated to toxin biosynthesis in the examined alga and may be used to serve as a potential toxin indicator. © 2005 WILEY-VCH Verlag GmbH & Co. KGaA.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subjectAlexandrium minutumen_HK
dc.subjectHarmful algal bloom speciesen_HK
dc.subjectParalytic shellfish poisoningen_HK
dc.subjectParalytic shellfish poisoning toxinen_HK
dc.subjectTwo-dimensional gel electrophoresisen_HK
dc.titleUse of two-dimensional gel electrophoresis to differentiate morphospecies of Alexandrium minutum, a paralytic shellfish poisoning toxin-producing dinoflagellate of harmful algal bloomsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9853&volume=5&spage=1580&epage=1593&date=2005&atitle=Use+of+two-dimensional+gel+electrophoresis+to+differentiate+morphospecies+of+Alexandrium+minutum,+a+paralytic+shellfish+poisoning+toxin-producing+dinoflagellate+of+harmful+algal+bloomsen_HK
dc.identifier.emailWan, JMF: jmfwan@hku.hken_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.200401020en_HK
dc.identifier.pmid15800974-
dc.identifier.scopuseid_2-s2.0-17844391843en_HK
dc.identifier.hkuros100002en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-17844391843&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1580en_HK
dc.identifier.epage1593en_HK
dc.identifier.isiWOS:000228772400011-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridChan, LL=35757489200en_HK
dc.identifier.scopusauthoridHodgkiss, IJ=7006614647en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridWan, JMF=8930305000en_HK
dc.identifier.scopusauthoridChou, HN=7202063704en_HK
dc.identifier.scopusauthoridLum, JHK=7005329380en_HK
dc.identifier.scopusauthoridLo, MGY=36899513700en_HK
dc.identifier.scopusauthoridMak, ASC=7103123348en_HK
dc.identifier.scopusauthoridSit, WH=8528923000en_HK
dc.identifier.scopusauthoridLo, SCL=7401542305en_HK

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