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Article: The human secretin receptor gene: Genomic organization and promoter characterization

TitleThe human secretin receptor gene: Genomic organization and promoter characterization
Authors
KeywordsAutism
Gene structure
Promoter study
Secretin
Secretin receptor
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 1999, v. 455 n. 3, p. 209-214 How to Cite?
AbstractSecretin is the most potent regulator of pancreatic bicarbonate, electrolyte and volume secretion. In this report, the organization of the human secretin receptor (hSR) gene was characterized by overlapping genomic phage clones. The hSR gene consists of 13 exons and 12 introns with all the splice donor and acceptor sites conforming to the canonical GT/AG rule. By transient reporter gene assays, the wild-type promoter, containing 3.0 kb of the hSR gene 5' flanking region, was able to drive 5.8±0.6 and 6.6±0.2-fold (P<0.01) increases in luciferase activities in pancreatic ductule-derived PANC-1 and BPD-1 cells, respectively. By subsequent 5' and 3' deletion analysis, a promoter element was identified within -408 to -158, relative to the ATG codon. This promoter element was found to be cell-specific since it could drive reporter gene expression in PANC-1 and BPD-1 cells but not in Hs 262.St, Hs 746T and αT3-1 cells. The study of the transcriptional control of human secretin and its receptor should shed light on the pathological developments of pancreatic cancer and autism in the future. Copyright (C) 1999 Federation of European Biochemical Societies.
Persistent Identifierhttp://hdl.handle.net/10722/84633
ISSN
2015 Impact Factor: 3.519
2015 SCImago Journal Rankings: 2.026
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, PKen_HK
dc.contributor.authorFong, RSMen_HK
dc.contributor.authorKai, HSTen_HK
dc.contributor.authorLau, EHYen_HK
dc.contributor.authorNgan, ESWen_HK
dc.contributor.authorCotton, CUen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:55:19Z-
dc.date.available2010-09-06T08:55:19Z-
dc.date.issued1999en_HK
dc.identifier.citationFebs Letters, 1999, v. 455 n. 3, p. 209-214en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84633-
dc.description.abstractSecretin is the most potent regulator of pancreatic bicarbonate, electrolyte and volume secretion. In this report, the organization of the human secretin receptor (hSR) gene was characterized by overlapping genomic phage clones. The hSR gene consists of 13 exons and 12 introns with all the splice donor and acceptor sites conforming to the canonical GT/AG rule. By transient reporter gene assays, the wild-type promoter, containing 3.0 kb of the hSR gene 5' flanking region, was able to drive 5.8±0.6 and 6.6±0.2-fold (P<0.01) increases in luciferase activities in pancreatic ductule-derived PANC-1 and BPD-1 cells, respectively. By subsequent 5' and 3' deletion analysis, a promoter element was identified within -408 to -158, relative to the ATG codon. This promoter element was found to be cell-specific since it could drive reporter gene expression in PANC-1 and BPD-1 cells but not in Hs 262.St, Hs 746T and αT3-1 cells. The study of the transcriptional control of human secretin and its receptor should shed light on the pathological developments of pancreatic cancer and autism in the future. Copyright (C) 1999 Federation of European Biochemical Societies.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.rightsF E B S Letters. Copyright © Elsevier BV.en_HK
dc.subjectAutismen_HK
dc.subjectGene structureen_HK
dc.subjectPromoter studyen_HK
dc.subjectSecretinen_HK
dc.subjectSecretin receptoren_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAutistic Disorder - geneticsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshChromosome Mappingen_HK
dc.subject.meshDNA - geneticsen_HK
dc.subject.meshDNA Probes - geneticsen_HK
dc.subject.meshExonsen_HK
dc.subject.meshGenes, Reporteren_HK
dc.subject.meshGenome, Humanen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntronsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshReceptors, G-Protein-Coupleden_HK
dc.subject.meshReceptors, Gastrointestinal Hormone - geneticsen_HK
dc.subject.meshSecretin - metabolismen_HK
dc.titleThe human secretin receptor gene: Genomic organization and promoter characterizationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-5793&volume=455&spage=209&epage=214&date=1999&atitle=The+human+secretin+receptor+gene:+genomic+organization+and+promoter+characterizationen_HK
dc.identifier.emailLau, EHY: ehylau@hku.hken_HK
dc.identifier.emailNgan, ESW: engan@hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityLau, EHY=rp01349en_HK
dc.identifier.authorityNgan, ESW=rp00422en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0014-5793(99)00864-9en_HK
dc.identifier.pmid10437774-
dc.identifier.scopuseid_2-s2.0-0032812191en_HK
dc.identifier.hkuros47968en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032812191&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume455en_HK
dc.identifier.issue3en_HK
dc.identifier.spage209en_HK
dc.identifier.epage214en_HK
dc.identifier.isiWOS:000081753500003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHo, PK=9244016100en_HK
dc.identifier.scopusauthoridFong, RSM=7005653492en_HK
dc.identifier.scopusauthoridKai, HST=36900417600en_HK
dc.identifier.scopusauthoridLau, EHY=7103086074en_HK
dc.identifier.scopusauthoridNgan, ESW=22234827500en_HK
dc.identifier.scopusauthoridCotton, CU=7007041679en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK

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