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Article: Functional studies of a glucagon receptor isolated from frog Rana tigrina rugulosa: Implications on the molecular evolution of glucagon receptors in vertebrates

TitleFunctional studies of a glucagon receptor isolated from frog Rana tigrina rugulosa: Implications on the molecular evolution of glucagon receptors in vertebrates
Authors
KeywordsFrog
Glucagon
Glucagon antagonist des-His 1-[Nle 9-Ala 11-Ala 16]
Glucagon receptor
Glucagon-like peptide 1
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 1999, v. 457 n. 3, p. 499-504 How to Cite?
AbstractIn this report, the first amphibian glucagon receptor (GluR) cDNA was characterized from the liver of the frog Rana tigrina rugulosa. Functional expression of the frog GluR in CHO and COS-7 cells showed a high specificity of the receptor towards human glucagon with an EC 50 value of 0.8±0.5 nM. The binding of radioiodinated human glucagon to GluR was displaced in a dose-dependent manner only with human glucagon and its antagonist (des-His 1-[Nle 9-Ala 11-Ala 16]) with IC 50 values of 12.0±3.0 and 7.8±1.0 nM, respectively. The frog GluR did not display any affinity towards fish and human GLP-1s, and towards glucagon peptides derived from two species of teleost fishes (goldfish, zebrafish). These fish glucagons contain substitutions in several key residues that were previously shown to be critical for the binding of human glucagon to its receptor. By RT-PCR, mRNA transcripts of frog GluR were located in the liver, brain, small intestine and colon. These results demonstrate a conservation of the functional characteristics of the GluRs in frog and mammalian species and provide a framework for a better understanding of the molecular evolution of the GluR and its physiological function in vertebrates. Copyright (C) 1999 Federation of European Biochemical Societies.
Persistent Identifierhttp://hdl.handle.net/10722/84629
ISSN
2015 Impact Factor: 3.519
2015 SCImago Journal Rankings: 2.026
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNgan, ESWen_HK
dc.contributor.authorChow, LSNen_HK
dc.contributor.authorTse, DLYen_HK
dc.contributor.authorDu, Xen_HK
dc.contributor.authorWei, Yen_HK
dc.contributor.authorMojsov, Sen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:55:16Z-
dc.date.available2010-09-06T08:55:16Z-
dc.date.issued1999en_HK
dc.identifier.citationFebs Letters, 1999, v. 457 n. 3, p. 499-504en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84629-
dc.description.abstractIn this report, the first amphibian glucagon receptor (GluR) cDNA was characterized from the liver of the frog Rana tigrina rugulosa. Functional expression of the frog GluR in CHO and COS-7 cells showed a high specificity of the receptor towards human glucagon with an EC 50 value of 0.8±0.5 nM. The binding of radioiodinated human glucagon to GluR was displaced in a dose-dependent manner only with human glucagon and its antagonist (des-His 1-[Nle 9-Ala 11-Ala 16]) with IC 50 values of 12.0±3.0 and 7.8±1.0 nM, respectively. The frog GluR did not display any affinity towards fish and human GLP-1s, and towards glucagon peptides derived from two species of teleost fishes (goldfish, zebrafish). These fish glucagons contain substitutions in several key residues that were previously shown to be critical for the binding of human glucagon to its receptor. By RT-PCR, mRNA transcripts of frog GluR were located in the liver, brain, small intestine and colon. These results demonstrate a conservation of the functional characteristics of the GluRs in frog and mammalian species and provide a framework for a better understanding of the molecular evolution of the GluR and its physiological function in vertebrates. Copyright (C) 1999 Federation of European Biochemical Societies.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.rightsF E B S Letters. Copyright © Elsevier BV.en_HK
dc.subjectFrogen_HK
dc.subjectGlucagonen_HK
dc.subjectGlucagon antagonist des-His 1-[Nle 9-Ala 11-Ala 16]en_HK
dc.subjectGlucagon receptoren_HK
dc.subjectGlucagon-like peptide 1en_HK
dc.titleFunctional studies of a glucagon receptor isolated from frog Rana tigrina rugulosa: Implications on the molecular evolution of glucagon receptors in vertebratesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-5793&volume=457&spage=499&epage=504&date=1999&atitle=Functional+studies+of+a+glucagon+receptor+isolated+from+frog+Rana+tigrina+rugulosa:+implications+on+the+molecular+evolution+of+glucagon+receptors+in+vertebratesen_HK
dc.identifier.emailNgan, ESW: engan@hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityNgan, ESW=rp00422en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0014-5793(99)01112-6en_HK
dc.identifier.pmid10471837-
dc.identifier.scopuseid_2-s2.0-0032867337en_HK
dc.identifier.hkuros47970en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032867337&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume457en_HK
dc.identifier.issue3en_HK
dc.identifier.spage499en_HK
dc.identifier.epage504en_HK
dc.identifier.isiWOS:000082454500043-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridNgan, ESW=22234827500en_HK
dc.identifier.scopusauthoridChow, LSN=7202533094en_HK
dc.identifier.scopusauthoridTse, DLY=7101916515en_HK
dc.identifier.scopusauthoridDu, X=36861888300en_HK
dc.identifier.scopusauthoridWei, Y=36856015300en_HK
dc.identifier.scopusauthoridMojsov, S=7004216736en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK

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