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Conference Paper: Application of RAPD markers for evaluation of genetic diversity in Spiranther sinensis and S. hongkongensis (Orchidaceae)

TitleApplication of RAPD markers for evaluation of genetic diversity in Spiranther sinensis and S. hongkongensis (Orchidaceae)
Authors
Issue Date1995
PublisherBotanical Society of America, Inc (Columbus), Business Office. The Journal's web site is located at http://www.amjbot.org/
Citation
The Annual Meeting of the Botanical Society of America with Other Affiliated Societies, San Diego, CA., 6-10 August 1995. How to Cite?
AbstractIn Hong Kong, urban development has rapidly encroached on many diverse natural habitats, which once supported more than one hundred species of wild orchids such as Spiranthes sinensis and S. hongkongensis. To estimate and conserve the existing genetic diversity in the wild orchids, the random amplified polymorphic DNA (RAPD) method was used for analyzing genetic diversity. A total of 329 distinct DNA fragments were amplified using 33 random ten-base synthetic oligonucleotides. The proportion of polymorphic loci was 64% in Spiranthes sinensis, and 17% in S. hongkongensis. The RAPD data is consistent with isozyme analysis, which revealed a very high level of genetic variation in populations of S. sinensis (P = 44.8%, A = 1.65, H = 0.17), but nearly complete lack of isozyme variation in populations of S. hongkongensis. Factors that may have contributed to this contrasting pattern of genetic diversity in the two species, and implications for orchid conservation, will be discussed
DescriptionAmerican Journal of Botany, 1995, v. 82 n. 6 Supp, p. 71 Abstract no. 201
http://www.jstor.org/stable/2445799
Persistent Identifierhttp://hdl.handle.net/10722/84626
ISSN
2015 Impact Factor: 2.811
2015 SCImago Journal Rankings: 1.459

 

DC FieldValueLanguage
dc.contributor.authorChan, MCen_HK
dc.contributor.authorSun, Men_HK
dc.date.accessioned2010-09-06T08:55:14Z-
dc.date.available2010-09-06T08:55:14Z-
dc.date.issued1995en_HK
dc.identifier.citationThe Annual Meeting of the Botanical Society of America with Other Affiliated Societies, San Diego, CA., 6-10 August 1995.en_HK
dc.identifier.issn0002-9122en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84626-
dc.descriptionAmerican Journal of Botany, 1995, v. 82 n. 6 Supp, p. 71 Abstract no. 201-
dc.descriptionhttp://www.jstor.org/stable/2445799-
dc.description.abstractIn Hong Kong, urban development has rapidly encroached on many diverse natural habitats, which once supported more than one hundred species of wild orchids such as Spiranthes sinensis and S. hongkongensis. To estimate and conserve the existing genetic diversity in the wild orchids, the random amplified polymorphic DNA (RAPD) method was used for analyzing genetic diversity. A total of 329 distinct DNA fragments were amplified using 33 random ten-base synthetic oligonucleotides. The proportion of polymorphic loci was 64% in Spiranthes sinensis, and 17% in S. hongkongensis. The RAPD data is consistent with isozyme analysis, which revealed a very high level of genetic variation in populations of S. sinensis (P = 44.8%, A = 1.65, H = 0.17), but nearly complete lack of isozyme variation in populations of S. hongkongensis. Factors that may have contributed to this contrasting pattern of genetic diversity in the two species, and implications for orchid conservation, will be discussed-
dc.languageengen_HK
dc.publisherBotanical Society of America, Inc (Columbus), Business Office. The Journal's web site is located at http://www.amjbot.org/en_HK
dc.relation.ispartofAmerican Journal of Botanyen_HK
dc.titleApplication of RAPD markers for evaluation of genetic diversity in Spiranther sinensis and S. hongkongensis (Orchidaceae)en_HK
dc.typeConference_Paperen_HK
dc.identifier.emailSun, M: meisun@hkucc.hku.hken_HK
dc.identifier.authoritySun, M=rp00779en_HK
dc.identifier.hkuros7330en_HK
dc.identifier.hkuros5784-
dc.identifier.volume82-
dc.identifier.issue6 Supp-
dc.identifier.spage71 Abstract no. 201-
dc.identifier.epage71 Abstract no. 201-

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