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Article: Rabbit sex hormone-binding globulin: Expression in the liver and testis during postnatal development and structural characterization by truncated proteins

TitleRabbit sex hormone-binding globulin: Expression in the liver and testis during postnatal development and structural characterization by truncated proteins
Authors
KeywordsPostnatal expression
Sex hormone binding globulin
Truncated SHBG
Issue Date2001
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJA
Citation
International Journal Of Andrology, 2001, v. 24 n. 3, p. 165-174 How to Cite?
AbstractAlthough sex hormone binding globulin (SHBG) is found in the blood plasma of adult humans and rabbits and the gene is expressed in their livers, it is not detected in the plasma of adult rodents nor is it expressed in adult rodent livers. Thus the rabbit represents a good model to study the metabolism and function of SHBG in the blood. We have used a cloned rabbit SHBG cDNA to detect mRNA expression in rabbits during the postnatal period, and to construct truncated SHBG proteins for structure/function analysis. The SHBG mRNA appeared in the testis as early as 3 days after birth. The level increased gradually in abundance throughout postnatal development, and attained a maximum at 12 weeks of age when the gonads were fully matured. In contrast, SHBG mRNA in the livers of male and female animals increased to a maximum by 4 weeks of age, and were maintained at this level until 12 weeks before subsiding to the initial levels. The increase and decrease in SHBG mRNA levels in the liver were accompanied by similar changes in serum SHBG. This suggests that SHBG in the blood circulation comes from the liver and this might also provide a source of SHBG for the male reproductive tract before formation of the blood-testis barrier. To elucidate the minimal sequence of rabbit SHBG responsible for steroid-binding, a panel of 13 truncated SHBG proteins was constructed, expressed in Escherichia coli, and biochemically purified for study. It was shown that the complete protein sequence of rabbit SHBG was important for maintaining a stable steroid-protein complex. Unlike human SHBG for which a truncated protein of the first 206 residues of the 373 amino acid protein can still bind steroid, removal of 43 or more residues from the C-terminus of rabbit SHBG completely abolished steroid-binding.
Persistent Identifierhttp://hdl.handle.net/10722/84619
ISSN
2014 Impact Factor: 3.695
2015 SCImago Journal Rankings: 1.161
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, ASTen_HK
dc.contributor.authorLui, WYen_HK
dc.contributor.authorHui, ITCen_HK
dc.contributor.authorLee, WMen_HK
dc.date.accessioned2010-09-06T08:55:09Z-
dc.date.available2010-09-06T08:55:09Z-
dc.date.issued2001en_HK
dc.identifier.citationInternational Journal Of Andrology, 2001, v. 24 n. 3, p. 165-174en_HK
dc.identifier.issn0105-6263en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84619-
dc.description.abstractAlthough sex hormone binding globulin (SHBG) is found in the blood plasma of adult humans and rabbits and the gene is expressed in their livers, it is not detected in the plasma of adult rodents nor is it expressed in adult rodent livers. Thus the rabbit represents a good model to study the metabolism and function of SHBG in the blood. We have used a cloned rabbit SHBG cDNA to detect mRNA expression in rabbits during the postnatal period, and to construct truncated SHBG proteins for structure/function analysis. The SHBG mRNA appeared in the testis as early as 3 days after birth. The level increased gradually in abundance throughout postnatal development, and attained a maximum at 12 weeks of age when the gonads were fully matured. In contrast, SHBG mRNA in the livers of male and female animals increased to a maximum by 4 weeks of age, and were maintained at this level until 12 weeks before subsiding to the initial levels. The increase and decrease in SHBG mRNA levels in the liver were accompanied by similar changes in serum SHBG. This suggests that SHBG in the blood circulation comes from the liver and this might also provide a source of SHBG for the male reproductive tract before formation of the blood-testis barrier. To elucidate the minimal sequence of rabbit SHBG responsible for steroid-binding, a panel of 13 truncated SHBG proteins was constructed, expressed in Escherichia coli, and biochemically purified for study. It was shown that the complete protein sequence of rabbit SHBG was important for maintaining a stable steroid-protein complex. Unlike human SHBG for which a truncated protein of the first 206 residues of the 373 amino acid protein can still bind steroid, removal of 43 or more residues from the C-terminus of rabbit SHBG completely abolished steroid-binding.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJAen_HK
dc.relation.ispartofInternational Journal of Andrologyen_HK
dc.rightsInternational Journal of Andrology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectPostnatal expressionen_HK
dc.subjectSex hormone binding globulinen_HK
dc.subjectTruncated SHBGen_HK
dc.titleRabbit sex hormone-binding globulin: Expression in the liver and testis during postnatal development and structural characterization by truncated proteinsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0105-6263&volume=24&spage=165&epage=174&date=2001&atitle=Rabbit+sex+hormone-binding+globulin:+expression+in+the+liver+and+testis+during+postnatal+development+and+structural+characterization+by+truncated+proteinsen_HK
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_HK
dc.identifier.emailLui, WY: wylui@hku.hken_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.authorityWong, AST=rp00805en_HK
dc.identifier.authorityLui, WY=rp00756en_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-2605.2001.00283.xen_HK
dc.identifier.pmid11380705-
dc.identifier.scopuseid_2-s2.0-0035012776en_HK
dc.identifier.hkuros56846en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035012776&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue3en_HK
dc.identifier.spage165en_HK
dc.identifier.epage174en_HK
dc.identifier.isiWOS:000168978700005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, AST=23987963300en_HK
dc.identifier.scopusauthoridLui, WY=35220192400en_HK
dc.identifier.scopusauthoridHui, ITC=7004838788en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK

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