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Article: Genetic structure of three orchid species with contrasting breeding systems using RAPD and allozyme markers

TitleGenetic structure of three orchid species with contrasting breeding systems using RAPD and allozyme markers
Authors
KeywordsAllozymes
Colonization
Eulophia sinensis
Genetic structure
Orchidaceae
RAPD
Zeuxine gracilis
Zeuxine strateumatica
Issue Date2001
PublisherBotanical Society of America, Inc (Columbus), Business Office. The Journal's web site is located at http://www.amjbot.org/
Citation
American Journal Of Botany, 2001, v. 88 n. 12, p. 2180-2188 How to Cite?
AbstractZeuxine gracilis, Zeuxine strateumatica, and Eulophia sinensis are wild orchids with different breeding systems and colonizing abilities. Zeuxine gracilis is an outcrosser with restricted distribution, whereas S. strateumatica is an apomictic colonizer found only in newly available open habitats. Eulophia sinensis is an outcrossing colonizer. This study investigates the levels of genetic variation and patterns of population structure in these wild orchids to provide genetic information for the development of suitable conservation strategies. Lack of allozyme variation was characteristic of all three species, especially in populations of the two colonizing orchids, Z. strateumatica and E. sinensis. More variable markers, randomly amplified polymorphic DNAs (RAPDs), were further employed to characterize population structure of these species. Substantial genetic variation was found at the RAPD loci within populations of Z. gracilis (p = 21.65 ± 15.88%, A = 1.217 ± 0.159, and H = 0.076 ± 0.054) and E. sinensis (p = 17.82 ± 20.97%, A = 1.179 ± 0.209, and H = 0.070 ± 0.084), but little variation existed within populations of Z. strateumatica (p = 2.84 ± 2.58%, A = 1.029 ± 0.026, and H = 0.011 ± 0.011). Regardless of the breeding system, the total gene diversity at the species level was partitioned primarily between populations, as shown by high GST, values, in all three species. An extremely high level of population differentiation (GST = 0.924) was found in the apomictic colonizer Z. strateumatica. The patterns of genetic variation in these wild orchids are apparently related to their differences in breeding system and colonizing ability. Different conservation strategies are needed for the long-term survival of these species.
Persistent Identifierhttp://hdl.handle.net/10722/84615
ISSN
2015 Impact Factor: 2.811
2015 SCImago Journal Rankings: 1.459
References

 

DC FieldValueLanguage
dc.contributor.authorSun, Men_HK
dc.contributor.authorWong, KCen_HK
dc.date.accessioned2010-09-06T08:55:06Z-
dc.date.available2010-09-06T08:55:06Z-
dc.date.issued2001en_HK
dc.identifier.citationAmerican Journal Of Botany, 2001, v. 88 n. 12, p. 2180-2188en_HK
dc.identifier.issn0002-9122en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84615-
dc.description.abstractZeuxine gracilis, Zeuxine strateumatica, and Eulophia sinensis are wild orchids with different breeding systems and colonizing abilities. Zeuxine gracilis is an outcrosser with restricted distribution, whereas S. strateumatica is an apomictic colonizer found only in newly available open habitats. Eulophia sinensis is an outcrossing colonizer. This study investigates the levels of genetic variation and patterns of population structure in these wild orchids to provide genetic information for the development of suitable conservation strategies. Lack of allozyme variation was characteristic of all three species, especially in populations of the two colonizing orchids, Z. strateumatica and E. sinensis. More variable markers, randomly amplified polymorphic DNAs (RAPDs), were further employed to characterize population structure of these species. Substantial genetic variation was found at the RAPD loci within populations of Z. gracilis (p = 21.65 ± 15.88%, A = 1.217 ± 0.159, and H = 0.076 ± 0.054) and E. sinensis (p = 17.82 ± 20.97%, A = 1.179 ± 0.209, and H = 0.070 ± 0.084), but little variation existed within populations of Z. strateumatica (p = 2.84 ± 2.58%, A = 1.029 ± 0.026, and H = 0.011 ± 0.011). Regardless of the breeding system, the total gene diversity at the species level was partitioned primarily between populations, as shown by high GST, values, in all three species. An extremely high level of population differentiation (GST = 0.924) was found in the apomictic colonizer Z. strateumatica. The patterns of genetic variation in these wild orchids are apparently related to their differences in breeding system and colonizing ability. Different conservation strategies are needed for the long-term survival of these species.en_HK
dc.languageengen_HK
dc.publisherBotanical Society of America, Inc (Columbus), Business Office. The Journal's web site is located at http://www.amjbot.org/en_HK
dc.relation.ispartofAmerican Journal of Botanyen_HK
dc.subjectAllozymesen_HK
dc.subjectColonizationen_HK
dc.subjectEulophia sinensisen_HK
dc.subjectGenetic structureen_HK
dc.subjectOrchidaceaeen_HK
dc.subjectRAPDen_HK
dc.subjectZeuxine gracilisen_HK
dc.subjectZeuxine strateumaticaen_HK
dc.titleGenetic structure of three orchid species with contrasting breeding systems using RAPD and allozyme markersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9122&volume=88&spage=2180&epage=2188&date=2001&atitle=Genetic+Structure+of+Three+Orchid+Species+with+Contrasting+Breeding+Systems+Using+RAPD+And+Allozyme+Markersen_HK
dc.identifier.emailSun, M: meisun@hkucc.hku.hken_HK
dc.identifier.authoritySun, M=rp00779en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0005875885en_HK
dc.identifier.hkuros66205en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0005875885&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume88en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2180en_HK
dc.identifier.epage2188en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSun, M=7403181447en_HK
dc.identifier.scopusauthoridWong, KC=7404759813en_HK

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