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Article: Molecular cloning of a cDNA encoding proglucagon from goldfish, Carassius auratus

TitleMolecular cloning of a cDNA encoding proglucagon from goldfish, Carassius auratus
Authors
KeywordsCarassius auratus
GLP
Glucagon
Glucagon-like peptide
Goldfish
Preproglucagon
Proglucagon
RACE
Rapid amplification of cDNA ends
Issue Date1997
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-1742
Citation
Fish Physiology And Biochemistry, 1997, v. 17 n. 1-6, p. 223-230 How to Cite?
AbstractProglucagon is the precursor for a number of peptides: glucagon and at least one glucagon-like peptide (GLP1 and GLP2). The production of these peptides is regulated by tissue-specific proteolytic processing. A cDNA encoding the proglucagon in goldfish was characterized using rapid amplification of the cDNA ends. Goldfish proglucagon cDNA was found to be 660 base pair long, encoding a putative 121-residue prepro-hormone. Goldfish glucagon and GLP do not terminate with two basic residues, as is common in vertebrates as specific endopeptidase cleavage recognition site. Instead, only one basic residue is found at these positions. It is not known whether goldfish glucagon and GLP have adopted an alternate processing pathway, or whether they remained unprocessed to yield C-terminal-extended peptides. By reverse transcriptase-polymerase chain reaction, the proglucagon mRNA was found to be expressed mainly in the gall bladder region and the proximal intestine, and less in the spleen and distal intestine.
Persistent Identifierhttp://hdl.handle.net/10722/84613
ISSN
2015 Impact Factor: 1.442
2015 SCImago Journal Rankings: 0.738
References

 

DC FieldValueLanguage
dc.contributor.authorYuen, TTHen_HK
dc.contributor.authorMok, PYen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:55:05Z-
dc.date.available2010-09-06T08:55:05Z-
dc.date.issued1997en_HK
dc.identifier.citationFish Physiology And Biochemistry, 1997, v. 17 n. 1-6, p. 223-230en_HK
dc.identifier.issn0920-1742en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84613-
dc.description.abstractProglucagon is the precursor for a number of peptides: glucagon and at least one glucagon-like peptide (GLP1 and GLP2). The production of these peptides is regulated by tissue-specific proteolytic processing. A cDNA encoding the proglucagon in goldfish was characterized using rapid amplification of the cDNA ends. Goldfish proglucagon cDNA was found to be 660 base pair long, encoding a putative 121-residue prepro-hormone. Goldfish glucagon and GLP do not terminate with two basic residues, as is common in vertebrates as specific endopeptidase cleavage recognition site. Instead, only one basic residue is found at these positions. It is not known whether goldfish glucagon and GLP have adopted an alternate processing pathway, or whether they remained unprocessed to yield C-terminal-extended peptides. By reverse transcriptase-polymerase chain reaction, the proglucagon mRNA was found to be expressed mainly in the gall bladder region and the proximal intestine, and less in the spleen and distal intestine.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-1742en_HK
dc.relation.ispartofFish Physiology and Biochemistryen_HK
dc.subjectCarassius auratusen_HK
dc.subjectGLPen_HK
dc.subjectGlucagonen_HK
dc.subjectGlucagon-like peptideen_HK
dc.subjectGoldfishen_HK
dc.subjectPreproglucagonen_HK
dc.subjectProglucagonen_HK
dc.subjectRACEen_HK
dc.subjectRapid amplification of cDNA endsen_HK
dc.titleMolecular cloning of a cDNA encoding proglucagon from goldfish, Carassius auratusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0920-1742&volume=17&spage=223&epage=230&date=1997&atitle=Molecular+cloning+of+a+cDNA+encoding+proglucagon+from+goldfish,+Carassius+auratusen_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0001589793en_HK
dc.identifier.hkuros24440en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0001589793&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue1-6en_HK
dc.identifier.spage223en_HK
dc.identifier.epage230en_HK
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridYuen, TTH=36957362600en_HK
dc.identifier.scopusauthoridMok, PY=36884813000en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK

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