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Article: Identification and purification of proteins from germ cell-conditioned medium (GCCM)

TitleIdentification and purification of proteins from germ cell-conditioned medium (GCCM)
Authors
KeywordsGerm cells
HPLC
Protein sequencing
Testis
Issue Date1999
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/15216543.asp
Citation
Biochemistry And Molecular Biology International, 1999, v. 47 n. 3, p. 479-491 How to Cite?
AbstractGerm cells are known to regulate Sertoli cell and testicular function possibly through released factor(s) or via cell-cell contact. However, the identities of many of these putative biological factors are not known. The aim of this study is to present a strategy to identify and purify germ cell derived proteins found in germ cell-conditioned medium (GCCM) at a quantity sufficient to permit protein microsequencing. The purification scheme of a novel germ cell-derived protein from GCCM designated GC-26 is presented along with several germ cell proteins using a combination of high pressure liquid chromatography (HPLC) columns. The purity of GC-26 and other germ cell proteins were confirmed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and silver staining. The identities of GC-26, a 26-kDa polypeptide, and other proteins were determined by direct protein microsequencing. These partial NH2-terminal amino acid sequences were compared with the existing databases at Protein Identification Resource (PIR), GenBank, and BLAST. These analyses revealed that these proteins are unique. This strategy should be useful for the micropurification of proteins from other biological samples and/or fluids.
Persistent Identifierhttp://hdl.handle.net/10722/84603
ISSN
2001 Impact Factor: 0.925
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChung, SSWen_HK
dc.contributor.authorMruk, Den_HK
dc.contributor.authorLee, WMen_HK
dc.contributor.authorCheng, CYen_HK
dc.date.accessioned2010-09-06T08:54:58Z-
dc.date.available2010-09-06T08:54:58Z-
dc.date.issued1999en_HK
dc.identifier.citationBiochemistry And Molecular Biology International, 1999, v. 47 n. 3, p. 479-491en_HK
dc.identifier.issn1039-9712en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84603-
dc.description.abstractGerm cells are known to regulate Sertoli cell and testicular function possibly through released factor(s) or via cell-cell contact. However, the identities of many of these putative biological factors are not known. The aim of this study is to present a strategy to identify and purify germ cell derived proteins found in germ cell-conditioned medium (GCCM) at a quantity sufficient to permit protein microsequencing. The purification scheme of a novel germ cell-derived protein from GCCM designated GC-26 is presented along with several germ cell proteins using a combination of high pressure liquid chromatography (HPLC) columns. The purity of GC-26 and other germ cell proteins were confirmed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and silver staining. The identities of GC-26, a 26-kDa polypeptide, and other proteins were determined by direct protein microsequencing. These partial NH2-terminal amino acid sequences were compared with the existing databases at Protein Identification Resource (PIR), GenBank, and BLAST. These analyses revealed that these proteins are unique. This strategy should be useful for the micropurification of proteins from other biological samples and/or fluids.en_HK
dc.languageengen_HK
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/15216543.aspen_HK
dc.relation.ispartofBiochemistry and Molecular Biology Internationalen_HK
dc.rightsBiochemistry and Molecular Biology International. Copyright © Informa Healthcare.en_HK
dc.subjectGerm cellsen_HK
dc.subjectHPLCen_HK
dc.subjectProtein sequencingen_HK
dc.subjectTestisen_HK
dc.titleIdentification and purification of proteins from germ cell-conditioned medium (GCCM)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1039-9712&volume=47&spage=479&epage=491&date=1999&atitle=Identification+and+purification+of+proteins+from+germ+cell-conditioned+medium+(GCCM)en_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10204085-
dc.identifier.scopuseid_2-s2.0-0032891770en_HK
dc.identifier.hkuros42370en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032891770&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue3en_HK
dc.identifier.spage479en_HK
dc.identifier.epage491en_HK
dc.identifier.isiWOS:000079512300015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChung, SSW=35104555300en_HK
dc.identifier.scopusauthoridMruk, D=6701823934en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK
dc.identifier.scopusauthoridCheng, CY=7404797787en_HK

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