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Article: Hepatic potential of bone marrow stromal cells: Development of in vitro co-culture and intra-portal transplantation models

TitleHepatic potential of bone marrow stromal cells: Development of in vitro co-culture and intra-portal transplantation models
Authors
KeywordsBone marrow
Cirrhosis
Hepatocyte
Mesenchymal stem cells
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim
Citation
Journal Of Immunological Methods, 2005, v. 305 n. 1, p. 39-47 How to Cite?
AbstractBone marrow comprises heterogeneous cell populations and is thought to contain certain progenitors with the ability to differentiate into multiple mesenchymal cell lineages. To identify any differentiation plasticity of adult bone marrow stromal cells (BMSCs) into hepatocyte-like phenotypes, we developed a co-culture model with damaged liver tissue and an animal model of engraftment in cirrhotic liver via intra-portal transplantation. After 10 days of co-culture with injured liver tissues, BMSC expressed specific markers for hepatocytes by RT-PCR and Western blot. The two animal models for liver injury employed used carbon tetrachloride (CCl4) induction or bile duct ligation. For tracing BMSC resident in the liver after intra-portal transplantation, green fluorescent protein (GFP)-positive BMSCs or in situ hybdization of Y-chromosome Sry gene in male BMSC were used in a cross-sex transplantation model. Expression of hepatocyte-specific markers in recipients' liver tissues was determined by fluorescence immunohistochemistry. Our findings demonstrated that about 16% parenchyma cells were GFP-positive cells derived from infused BMSCs, and expression of albumin was detected in these cells in engrafted liver tissues. In conclusion, BMSCs exhibited hepatocyte-like phenotypes after co-cultivation with liver tissue and transplanted into the injured liver. The presented evidence indicated the trans differentiation potential of BMSC developing to the hepatocytes. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/84457
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 1.053
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorWang, PPen_HK
dc.contributor.authorLee, CKen_HK
dc.contributor.authorWang, JHen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-06T08:53:11Z-
dc.date.available2010-09-06T08:53:11Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Immunological Methods, 2005, v. 305 n. 1, p. 39-47en_HK
dc.identifier.issn0022-1759en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84457-
dc.description.abstractBone marrow comprises heterogeneous cell populations and is thought to contain certain progenitors with the ability to differentiate into multiple mesenchymal cell lineages. To identify any differentiation plasticity of adult bone marrow stromal cells (BMSCs) into hepatocyte-like phenotypes, we developed a co-culture model with damaged liver tissue and an animal model of engraftment in cirrhotic liver via intra-portal transplantation. After 10 days of co-culture with injured liver tissues, BMSC expressed specific markers for hepatocytes by RT-PCR and Western blot. The two animal models for liver injury employed used carbon tetrachloride (CCl4) induction or bile duct ligation. For tracing BMSC resident in the liver after intra-portal transplantation, green fluorescent protein (GFP)-positive BMSCs or in situ hybdization of Y-chromosome Sry gene in male BMSC were used in a cross-sex transplantation model. Expression of hepatocyte-specific markers in recipients' liver tissues was determined by fluorescence immunohistochemistry. Our findings demonstrated that about 16% parenchyma cells were GFP-positive cells derived from infused BMSCs, and expression of albumin was detected in these cells in engrafted liver tissues. In conclusion, BMSCs exhibited hepatocyte-like phenotypes after co-cultivation with liver tissue and transplanted into the injured liver. The presented evidence indicated the trans differentiation potential of BMSC developing to the hepatocytes. © 2005 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jimen_HK
dc.relation.ispartofJournal of Immunological Methodsen_HK
dc.rightsJournal of Immunological Methods. Copyright © Elsevier BV.en_HK
dc.subjectBone marrowen_HK
dc.subjectCirrhosisen_HK
dc.subjectHepatocyteen_HK
dc.subjectMesenchymal stem cellsen_HK
dc.titleHepatic potential of bone marrow stromal cells: Development of in vitro co-culture and intra-portal transplantation modelsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1759&volume=305&issue=1&spage=39&epage=47&date=2005&atitle=Hepatic+potential+of+bone+marrow+stromal+cells:+development+of+in+vitro+co-culture+and+intra-portal+transplantation+modelsen_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jim.2005.07.006en_HK
dc.identifier.pmid16150456en_HK
dc.identifier.scopuseid_2-s2.0-26244435756en_HK
dc.identifier.hkuros116938en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-26244435756&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume305en_HK
dc.identifier.issue1en_HK
dc.identifier.spage39en_HK
dc.identifier.epage47en_HK
dc.identifier.isiWOS:000232814400006-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridWang, PP=7405460611en_HK
dc.identifier.scopusauthoridLee, CK=8220822700en_HK
dc.identifier.scopusauthoridWang, JH=8898010700en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK

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