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Article: Triptolide, a component of Chinese herbal medicine, modulates the functional phenotype of dendritic cells

TitleTriptolide, a component of Chinese herbal medicine, modulates the functional phenotype of dendritic cells
Authors
KeywordsCardiac allografts
Dendritic cells
Immunosuppression
Regulatory T cells
Triptolide
Issue Date2007
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.transplantjournal.com
Citation
Transplantation, 2007, v. 84 n. 11, p. 1517-1526 How to Cite?
AbstractBACKGROUND. Triptolide (TPT) is a component of the Chinese herb Triptergium wilfordii and has potent immunosuppressive and anti-inflammatory effects. Since dendritic cells (DCs) play a critical role in the initiation of alloimmune responses to foreign grafts, the aim of the present study was to evaluate the effects of TPT in the functional phenotype of bone marrow (BM)-derived DCs. METHODS. BM-derived DCs were cultured with or without TPT from days 2 to 7 and then stimulated with lipopolysaccharide (LPS) for a further 2 days. In some experiments, TPT was added to the cultures from day 7 to day 9. Heterotopic cardiac transplantation was performed and animals received either no treatment or were injected systemically with different doses of TPT posttransplantation. RESULTS. TPT treatment inhibited LPS-induced DC maturation. The ability of DCs to stimulate allogeneic T-cell responses was also impaired by TPT treatment and accompanied by upregulated synthesis of interleukin-10 facilitating the expansion of regulatory T cells. Furthermore, we observed that TPT treatment led to increased cell surface expression of DC-SIGN and reduced expression of TLR4 on DCs. CONCLUSIONS. These findings demonstrate that TPT can inhibit the maturation and allogenicity of DCs and promotes the expansion of regulatory T cells. These effects were confirmed by in vivo experiments though treatment of TPT can prolong mice heart allograft survival. Furthermore, modulation of the DC surface phenotype towards that of a tolerogenic phenotype could contribute to the ability of TPT to suppress productive immunity and maintain homeostasis. © 2007 Lippincott Williams & Wilkins, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/84428
ISSN
2015 Impact Factor: 3.69
2015 SCImago Journal Rankings: 1.699
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorLamb, JRen_HK
dc.contributor.authorTam, PKHen_HK
dc.date.accessioned2010-09-06T08:52:51Z-
dc.date.available2010-09-06T08:52:51Z-
dc.date.issued2007en_HK
dc.identifier.citationTransplantation, 2007, v. 84 n. 11, p. 1517-1526en_HK
dc.identifier.issn0041-1337en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84428-
dc.description.abstractBACKGROUND. Triptolide (TPT) is a component of the Chinese herb Triptergium wilfordii and has potent immunosuppressive and anti-inflammatory effects. Since dendritic cells (DCs) play a critical role in the initiation of alloimmune responses to foreign grafts, the aim of the present study was to evaluate the effects of TPT in the functional phenotype of bone marrow (BM)-derived DCs. METHODS. BM-derived DCs were cultured with or without TPT from days 2 to 7 and then stimulated with lipopolysaccharide (LPS) for a further 2 days. In some experiments, TPT was added to the cultures from day 7 to day 9. Heterotopic cardiac transplantation was performed and animals received either no treatment or were injected systemically with different doses of TPT posttransplantation. RESULTS. TPT treatment inhibited LPS-induced DC maturation. The ability of DCs to stimulate allogeneic T-cell responses was also impaired by TPT treatment and accompanied by upregulated synthesis of interleukin-10 facilitating the expansion of regulatory T cells. Furthermore, we observed that TPT treatment led to increased cell surface expression of DC-SIGN and reduced expression of TLR4 on DCs. CONCLUSIONS. These findings demonstrate that TPT can inhibit the maturation and allogenicity of DCs and promotes the expansion of regulatory T cells. These effects were confirmed by in vivo experiments though treatment of TPT can prolong mice heart allograft survival. Furthermore, modulation of the DC surface phenotype towards that of a tolerogenic phenotype could contribute to the ability of TPT to suppress productive immunity and maintain homeostasis. © 2007 Lippincott Williams & Wilkins, Inc.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.transplantjournal.comen_HK
dc.relation.ispartofTransplantationen_HK
dc.rightsTransplantation. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subjectCardiac allograftsen_HK
dc.subjectDendritic cellsen_HK
dc.subjectImmunosuppressionen_HK
dc.subjectRegulatory T cellsen_HK
dc.subjectTriptolideen_HK
dc.titleTriptolide, a component of Chinese herbal medicine, modulates the functional phenotype of dendritic cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0041-1337&volume=84&issue=11&spage=1517&epage=1526&date=2007&atitle=Triptolide,+a+component+of+Chinese+herbal+medicine,+modulates+the+functional+phenotype+of+dendritic+cellsen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.emailTam, PKH:paultam@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp01318en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/01.tp.0000289990.55668.0den_HK
dc.identifier.pmid18091529-
dc.identifier.scopuseid_2-s2.0-37349061751en_HK
dc.identifier.hkuros139567en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-37349061751&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume84en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1517en_HK
dc.identifier.epage1526en_HK
dc.identifier.isiWOS:000251681800019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, Y=25928027200en_HK
dc.identifier.scopusauthoridChen, Y=36463185300en_HK
dc.identifier.scopusauthoridLamb, JR=7201524642en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK

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