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Article: Matrix metalloproteinase-9 is differentially expressed in nonfunctioning invasive and noninvasive pituitary adenomas and increases invasion in human pituitary adenoma cell line

TitleMatrix metalloproteinase-9 is differentially expressed in nonfunctioning invasive and noninvasive pituitary adenomas and increases invasion in human pituitary adenoma cell line
Authors
Issue Date2007
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 2007, v. 170 n. 1, p. 356-365 How to Cite?
AbstractThe complete resection of pituitary adenomas (PAs) is unlikely when there is an extensive local dural invasion and given that the molecular mechanisms remain primarily unknown. DNA microarray analysis was performed to identify differentially expressed genes between nonfunctioning invasive and noninvasive PAs. Gene clustering revealed a robust eightfold increase in matrix metalloproteinase (MMP)-9 expression in surgically resected human invasive PAs and in the (nonfunctioning) HP75 human pituitary tumor-derived cell line treated with phorbol-12-myristate-13-acetate; these results were confirmed by real-time polymerase chain reaction, gelatin zymography, reverse transcriptase-polymerase chain reaction, Western blot, immunohistochemistry, and Northern blot analyses. The activation of protein kinase C (PKC) increased both MMP-9 activity and expression, which were blocked by some PKC inhibitors (G66976, bisindolylmaleimide, and Rottlerin), PKC-α, and PKC-δ small interfering (si)RNAs but not by hispidin (PKC-β inhibitor). In a transmembrane invasion assay, phorbol-12-myristate-13-acetate (100 nmol/L) increased the number of invaded HP75 cells, a process that was attenuated by PKC inhibitors, MMP-9 antibody, PKC-α siRNA, or PKC-δ siRNA. These results demonstrate that MMP-9 and PKC-α or PRC-δ may provide putative therapeutic targets for the control of PA dural invasion. Copyright © American Society for Investigative Pathology.
Persistent Identifierhttp://hdl.handle.net/10722/84259
ISSN
2015 Impact Factor: 4.206
2015 SCImago Journal Rankings: 2.653
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHussaini, IMen_HK
dc.contributor.authorTrotter, Cen_HK
dc.contributor.authorZhao, Yen_HK
dc.contributor.authorAbdelFattah, Ren_HK
dc.contributor.authorAmos, Sen_HK
dc.contributor.authorXiao, Aen_HK
dc.contributor.authorAgi, CUen_HK
dc.contributor.authorRedpath, GTen_HK
dc.contributor.authorFang, Zen_HK
dc.contributor.authorLeung, GKKen_HK
dc.contributor.authorLopes, MBSen_HK
dc.contributor.authorLaws Jr, ERen_HK
dc.date.accessioned2010-09-06T08:50:50Z-
dc.date.available2010-09-06T08:50:50Z-
dc.date.issued2007en_HK
dc.identifier.citationAmerican Journal Of Pathology, 2007, v. 170 n. 1, p. 356-365en_HK
dc.identifier.issn0002-9440en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84259-
dc.description.abstractThe complete resection of pituitary adenomas (PAs) is unlikely when there is an extensive local dural invasion and given that the molecular mechanisms remain primarily unknown. DNA microarray analysis was performed to identify differentially expressed genes between nonfunctioning invasive and noninvasive PAs. Gene clustering revealed a robust eightfold increase in matrix metalloproteinase (MMP)-9 expression in surgically resected human invasive PAs and in the (nonfunctioning) HP75 human pituitary tumor-derived cell line treated with phorbol-12-myristate-13-acetate; these results were confirmed by real-time polymerase chain reaction, gelatin zymography, reverse transcriptase-polymerase chain reaction, Western blot, immunohistochemistry, and Northern blot analyses. The activation of protein kinase C (PKC) increased both MMP-9 activity and expression, which were blocked by some PKC inhibitors (G66976, bisindolylmaleimide, and Rottlerin), PKC-α, and PKC-δ small interfering (si)RNAs but not by hispidin (PKC-β inhibitor). In a transmembrane invasion assay, phorbol-12-myristate-13-acetate (100 nmol/L) increased the number of invaded HP75 cells, a process that was attenuated by PKC inhibitors, MMP-9 antibody, PKC-α siRNA, or PKC-δ siRNA. These results demonstrate that MMP-9 and PKC-α or PRC-δ may provide putative therapeutic targets for the control of PA dural invasion. Copyright © American Society for Investigative Pathology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_HK
dc.relation.ispartofAmerican Journal of Pathologyen_HK
dc.titleMatrix metalloproteinase-9 is differentially expressed in nonfunctioning invasive and noninvasive pituitary adenomas and increases invasion in human pituitary adenoma cell lineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9440&volume=170&issue=1&spage=356&epage=365&date=2007&atitle=Matrix+metalloproteinase-9+is+differentially+expressed+in+nonfunctioning+invasive+and+noninvasive+pituitary+adenomas+and+increases+invasion+in+human+pituitary+adenoma+cell+lineen_HK
dc.identifier.emailLeung, GKK: gilberto@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GKK=rp00522en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2353/ajpath.2007.060736en_HK
dc.identifier.scopuseid_2-s2.0-33847037173en_HK
dc.identifier.hkuros125626en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33847037173&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume170en_HK
dc.identifier.issue1en_HK
dc.identifier.spage356en_HK
dc.identifier.epage365en_HK
dc.identifier.isiWOS:000243242900031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHussaini, IM=7003446454en_HK
dc.identifier.scopusauthoridTrotter, C=35968616000en_HK
dc.identifier.scopusauthoridZhao, Y=15849900000en_HK
dc.identifier.scopusauthoridAbdelFattah, R=15847758400en_HK
dc.identifier.scopusauthoridAmos, S=7005337861en_HK
dc.identifier.scopusauthoridXiao, A=15849922700en_HK
dc.identifier.scopusauthoridAgi, CU=15847641200en_HK
dc.identifier.scopusauthoridRedpath, GT=6603257331en_HK
dc.identifier.scopusauthoridFang, Z=36865107700en_HK
dc.identifier.scopusauthoridLeung, GKK=35965118200en_HK
dc.identifier.scopusauthoridLopes, MBS=35479962200en_HK
dc.identifier.scopusauthoridLaws Jr, ER=25950075000en_HK
dc.identifier.citeulike1070562-

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