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Article: EBV specific antibody-based and DNA-based assays in serologic diagnosis of nasopharyngeal carcinoma

TitleEBV specific antibody-based and DNA-based assays in serologic diagnosis of nasopharyngeal carcinoma
Authors
KeywordsDiagnosis
EBV antibody
NPC
Issue Date2003
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2003, v. 105 n. 5, p. 706-709 How to Cite?
AbstractWe assessed 5 EBV specific assays for their capacity to effect serologic diagnosis of suspected N PC. The assays were the immunofluorescent assays, VCA IgA and EA IgA, the enzyme-linked immunosorbent assays specific for EBNA I IgA or zta IgG and an EBV DNA assay. Serum samples were taken from 218 symptomatic NPC patients presenting consecutively at a public hospital in Hong Kong, 51 of whom were subsequently diagnosed as having NPC; 4 had EBV-associated lung cancer with similar serology as NPC. The remaining patients included 23 who had other cancers and 140 who had other diseases. Objectives of serodiagnosis under such clinical settings, therefore, are to both exclude and predict a diagnosis of NPC. None of the assays individually can meet both requirements adequately, however. The difficulty was best overcome by combining EBNA IIgA and zta IgG. It was shown that 68.3% of the patients gave a confirmed test results, negative or positive, by both tests. A confirmed negative result was associated with a negative predictive value of 99.1%, providing a clear indication to exclude a diagnosis of NPC; a confirmed positive result was associated with a positive predictive value of 86.8%, providing a clear indication to proceed with diagnostic work-up of NPC. The remaining patients gave equivocal test results, being positive for one or the other test, which were associated with a positive predictive value of 43.3% and 24.2%, respectively. © 2003 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/84091
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, KHen_HK
dc.contributor.authorGu, YLen_HK
dc.contributor.authorNg, Fen_HK
dc.contributor.authorNg, PSPen_HK
dc.contributor.authorSeto, WHen_HK
dc.contributor.authorSham, JSTen_HK
dc.contributor.authorChua, Den_HK
dc.contributor.authorWei, Wen_HK
dc.contributor.authorChen, YLen_HK
dc.contributor.authorLuk, Wen_HK
dc.contributor.authorZong, YSen_HK
dc.contributor.authorNg, MHen_HK
dc.date.accessioned2010-09-06T08:48:50Z-
dc.date.available2010-09-06T08:48:50Z-
dc.date.issued2003en_HK
dc.identifier.citationInternational Journal Of Cancer, 2003, v. 105 n. 5, p. 706-709en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84091-
dc.description.abstractWe assessed 5 EBV specific assays for their capacity to effect serologic diagnosis of suspected N PC. The assays were the immunofluorescent assays, VCA IgA and EA IgA, the enzyme-linked immunosorbent assays specific for EBNA I IgA or zta IgG and an EBV DNA assay. Serum samples were taken from 218 symptomatic NPC patients presenting consecutively at a public hospital in Hong Kong, 51 of whom were subsequently diagnosed as having NPC; 4 had EBV-associated lung cancer with similar serology as NPC. The remaining patients included 23 who had other cancers and 140 who had other diseases. Objectives of serodiagnosis under such clinical settings, therefore, are to both exclude and predict a diagnosis of NPC. None of the assays individually can meet both requirements adequately, however. The difficulty was best overcome by combining EBNA IIgA and zta IgG. It was shown that 68.3% of the patients gave a confirmed test results, negative or positive, by both tests. A confirmed negative result was associated with a negative predictive value of 99.1%, providing a clear indication to exclude a diagnosis of NPC; a confirmed positive result was associated with a positive predictive value of 86.8%, providing a clear indication to proceed with diagnostic work-up of NPC. The remaining patients gave equivocal test results, being positive for one or the other test, which were associated with a positive predictive value of 43.3% and 24.2%, respectively. © 2003 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectDiagnosisen_HK
dc.subjectEBV antibodyen_HK
dc.subjectNPCen_HK
dc.titleEBV specific antibody-based and DNA-based assays in serologic diagnosis of nasopharyngeal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=105&spage=706&epage=709&date=2003&atitle=EBV+specific+antibody-based+and+DNA-based+assays+in+serologic+diagnosis+of+nasopharyngeal+carcinomaen_HK
dc.identifier.emailChua, D: dttchua@hkucc.hku.hken_HK
dc.identifier.emailWei, W: hrmswwi@hku.hken_HK
dc.identifier.authorityChua, D=rp00415en_HK
dc.identifier.authorityWei, W=rp00323en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/ijc.11130en_HK
dc.identifier.pmid12740922-
dc.identifier.scopuseid_2-s2.0-0037499495en_HK
dc.identifier.hkuros79480en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037499495&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume105en_HK
dc.identifier.issue5en_HK
dc.identifier.spage706en_HK
dc.identifier.epage709en_HK
dc.identifier.isiWOS:000183474000020-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridGu, YL=7403046300en_HK
dc.identifier.scopusauthoridNg, F=7103125273en_HK
dc.identifier.scopusauthoridNg, PSP=36658517300en_HK
dc.identifier.scopusauthoridSeto, WH=7005799377en_HK
dc.identifier.scopusauthoridSham, JST=7101655565en_HK
dc.identifier.scopusauthoridChua, D=7006773480en_HK
dc.identifier.scopusauthoridWei, W=7403321552en_HK
dc.identifier.scopusauthoridChen, YL=7601438548en_HK
dc.identifier.scopusauthoridLuk, W=7005237837en_HK
dc.identifier.scopusauthoridZong, YS=7005203474en_HK
dc.identifier.scopusauthoridNg, MH=7202076421en_HK

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