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Article: Expression of hepatocyte-like phenotypes in bone marrow stromal cells after HGF induction

TitleExpression of hepatocyte-like phenotypes in bone marrow stromal cells after HGF induction
Authors
Keywordsα-Fetoprotein
Albumin
Bone marrow stromal cells
Hepatocyte growth factor
Mesenchymal stem cells
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2004, v. 320 n. 3, p. 712-716 How to Cite?
AbstractBone marrow comprises heterogeneous cell populations, of which certain progenitors have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. This study demonstrates the bone marrow stromal cells (BMSCs) with intrinsic plasticity to differentiate into hepatocyte-like phenotypes under in vitro induction of hepatocyte growth factor (HGF). BMSCs isolated from rat femurs and tibias were cultured and passaged 3-4 times in the presence of HGF. Cells were harvested on days 0, 10, and 20 and subjected to examination of any hepatocyte characteristics by flow cytometry, RT-PCR, Western blot, and immunocytochemistry. Expression of albumin and α-fetoprotein at both mRNA and protein levels was detectable on day 10. By contrast, c-Met mRNA was significantly decreased in BMSC in the course of HGF induction. Here BMSC was shown to differentiate into hepatocyte-like phenotypes given the HGF induction, as an alternative source for adult stem cell transplantation in liver repair. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/84048
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, PPen_HK
dc.contributor.authorWang, JHen_HK
dc.contributor.authorYan, ZPen_HK
dc.contributor.authorHu, MYen_HK
dc.contributor.authorLau, GKen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorLuk, JMen_HK
dc.date.accessioned2010-09-06T08:48:18Z-
dc.date.available2010-09-06T08:48:18Z-
dc.date.issued2004en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2004, v. 320 n. 3, p. 712-716en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84048-
dc.description.abstractBone marrow comprises heterogeneous cell populations, of which certain progenitors have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. This study demonstrates the bone marrow stromal cells (BMSCs) with intrinsic plasticity to differentiate into hepatocyte-like phenotypes under in vitro induction of hepatocyte growth factor (HGF). BMSCs isolated from rat femurs and tibias were cultured and passaged 3-4 times in the presence of HGF. Cells were harvested on days 0, 10, and 20 and subjected to examination of any hepatocyte characteristics by flow cytometry, RT-PCR, Western blot, and immunocytochemistry. Expression of albumin and α-fetoprotein at both mRNA and protein levels was detectable on day 10. By contrast, c-Met mRNA was significantly decreased in BMSC in the course of HGF induction. Here BMSC was shown to differentiate into hepatocyte-like phenotypes given the HGF induction, as an alternative source for adult stem cell transplantation in liver repair. © 2004 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectα-Fetoproteinen_HK
dc.subjectAlbuminen_HK
dc.subjectBone marrow stromal cellsen_HK
dc.subjectHepatocyte growth factoren_HK
dc.subjectMesenchymal stem cellsen_HK
dc.titleExpression of hepatocyte-like phenotypes in bone marrow stromal cells after HGF inductionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=320&issue=3&spage=712&epage=716&date=2004&atitle=Expression+of+hepatocyte-like+phenotypes+in+bone+marrow+stromal+cells+after+HGF+inductionen_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2004.05.213en_HK
dc.identifier.pmid15240106en_HK
dc.identifier.scopuseid_2-s2.0-3042841932en_HK
dc.identifier.hkuros90690en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3042841932&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume320en_HK
dc.identifier.issue3en_HK
dc.identifier.spage712en_HK
dc.identifier.epage716en_HK
dc.identifier.isiWOS:000222723200013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, PP=7405460611en_HK
dc.identifier.scopusauthoridWang, JH=8898010700en_HK
dc.identifier.scopusauthoridYan, ZP=7402519656en_HK
dc.identifier.scopusauthoridHu, MY=7402639252en_HK
dc.identifier.scopusauthoridLau, GK=7102301257en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK

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