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Article: Detection of C. pneumoniae by polymerase chain reaction-enzyme immunoassay in abdominal aortic aneurysm walls and its association with rupture

TitleDetection of C. pneumoniae by polymerase chain reaction-enzyme immunoassay in abdominal aortic aneurysm walls and its association with rupture
Authors
KeywordsAbdominal aortic aneurysm
C. pneumoniae
DNA
PCR-EIA
Rupture
Issue Date2005
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ejvs
Citation
European Journal Of Vascular And Endovascular Surgery, 2005, v. 29 n. 2, p. 150-155 How to Cite?
AbstractObjective: Serological studies have suggested that one of the risk factors for aneurysm development is C. pneumoniae infection. The purpose of this study was to evaluate whether there is an association between the presence of C. pneumoniae DNA in aneurysms and ruptured Abdominal Aortic Aneurysms. Methods: Aortic walls were collected consecutively from 30 patients with intact AAA, 16 patients with ruptured AAA and 19 healthy organ donors (control). Purified DNAs from all aortas were analyzed for the presence of C. pneumoniae DNA in parallel by polymerase chain reaction-enzyme immunoassay (PCR-EIA) and agarose gel electrophoresis. PCR-EIA has a high sensitivity in detecting low DNA copy number in clinical atherosclerotic samples. Results: C. pneumoniae DNA was detected more frequently in patients with aneurysms, particular with ruptured aneurysms. The incidence of positive C. pneumoniae DNA was 73.3% in intact AAA and 10.5% in control aortas, with the highest frequency in ruptured AAA (100%) (p<0.05). Conclusion: Giving the high specificity and sensitivity of PCR-EIA, these findings support the association of C. pneumoniae in the pathogenesis of aneurysm development, growth and rupture. © 2004 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/83979
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.723
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheuk, BLYen_HK
dc.contributor.authorTing, ACWen_HK
dc.contributor.authorCheng, SWKen_HK
dc.date.accessioned2010-09-06T08:47:29Z-
dc.date.available2010-09-06T08:47:29Z-
dc.date.issued2005en_HK
dc.identifier.citationEuropean Journal Of Vascular And Endovascular Surgery, 2005, v. 29 n. 2, p. 150-155en_HK
dc.identifier.issn1078-5884en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83979-
dc.description.abstractObjective: Serological studies have suggested that one of the risk factors for aneurysm development is C. pneumoniae infection. The purpose of this study was to evaluate whether there is an association between the presence of C. pneumoniae DNA in aneurysms and ruptured Abdominal Aortic Aneurysms. Methods: Aortic walls were collected consecutively from 30 patients with intact AAA, 16 patients with ruptured AAA and 19 healthy organ donors (control). Purified DNAs from all aortas were analyzed for the presence of C. pneumoniae DNA in parallel by polymerase chain reaction-enzyme immunoassay (PCR-EIA) and agarose gel electrophoresis. PCR-EIA has a high sensitivity in detecting low DNA copy number in clinical atherosclerotic samples. Results: C. pneumoniae DNA was detected more frequently in patients with aneurysms, particular with ruptured aneurysms. The incidence of positive C. pneumoniae DNA was 73.3% in intact AAA and 10.5% in control aortas, with the highest frequency in ruptured AAA (100%) (p<0.05). Conclusion: Giving the high specificity and sensitivity of PCR-EIA, these findings support the association of C. pneumoniae in the pathogenesis of aneurysm development, growth and rupture. © 2004 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ejvsen_HK
dc.relation.ispartofEuropean Journal of Vascular and Endovascular Surgeryen_HK
dc.subjectAbdominal aortic aneurysmen_HK
dc.subjectC. pneumoniaeen_HK
dc.subjectDNAen_HK
dc.subjectPCR-EIAen_HK
dc.subjectRuptureen_HK
dc.titleDetection of C. pneumoniae by polymerase chain reaction-enzyme immunoassay in abdominal aortic aneurysm walls and its association with ruptureen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1078-5884&volume=29&issue=2&spage=150&epage=155&date=2005&atitle=Detection+of+C.+pneumoniae+by+polymerase+chain+reaction-enzyme+immunoassay+in+abdominal+aortic+aneurysm+walls+and+its+association+with+ruptureen_HK
dc.identifier.emailCheuk, BLY: bernice@hku.hken_HK
dc.identifier.emailCheng, SWK: wkcheng@hkucc.hku.hken_HK
dc.identifier.authorityCheuk, BLY=rp01671en_HK
dc.identifier.authorityCheng, SWK=rp00374en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ejvs.2004.10.010en_HK
dc.identifier.pmid15649721-
dc.identifier.scopuseid_2-s2.0-12144281682en_HK
dc.identifier.hkuros97212en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-12144281682&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue2en_HK
dc.identifier.spage150en_HK
dc.identifier.epage155en_HK
dc.identifier.isiWOS:000226718000008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridCheuk, BLY=7801343617en_HK
dc.identifier.scopusauthoridTing, ACW=7102858552en_HK
dc.identifier.scopusauthoridCheng, SWK=7404684779en_HK

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