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Article: Antiinflammatory properties of IL-10 rescue small-for-size liver grafts
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TitleAntiinflammatory properties of IL-10 rescue small-for-size liver grafts
 
AuthorsYang, ZF1 2
Ho, DWY1
Ngai, P1
Lau, CK1
Zhao, Y1
Poon, RTP1
Fan, ST1
 
Issue Date2007
 
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021
 
CitationLiver Transplantation, 2007, v. 13 n. 4, p. 558-565 [How to Cite?]
DOI: http://dx.doi.org/10.1002/lt.21094
 
AbstractThe present study aims to investigate the potential therapeutic role of interleukin-10 (IL-10) in small-for-size liver transplantation. A syngenic rat orthotopic liver transplantation model was performed using either whole or 40% liver volume of Lewis rats as grafts according to the experimental design. IL-10 was given to the 40% grafts right after reperfusion, and also at 24 and 48 hours after transplantation. When no treatment was given, less than 40% of the small-for-size grafts survived indefinitely, whereas IL-10 treatment could increase the long-term survival rate of the small-for-size grafts to 80%. The 40% grafts presented with extensive areas of necrosis and increased number of apoptotic cells at the early phases after reperfusion. In addition, upregulation of plasma protein carbonyl content (PCC) levels was also detected in the 40% graft group. IL-10 treatment suppressed the upregulation of allograft inflammatory factor-1 (AIF-1) on macrophages in the 40% grafts, and at the same time, decreased the levels of plasma PCC, and improved the histology and function of the 40% grafts. The expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, and caspase 9 in the 40% grafts were upregulated after reperfusion, whereas the augmentation could be suppressed by the administration of IL-10. Finally, IL-10 culture could block AIF-1-mediated NO production and downregulate the expression of iNOS and TNF-α in a macrophage cell line. In conclusion, IL-10 rescued the small-for-size liver grafts by its antiinflammatory properties, through inhibition of AIF-1 mediated proinflammatory and proapoptotic activities of the macrophages during the early period after ischemia/reperfusion. © 2007 AASLD.
 
ISSN1527-6465
2013 Impact Factor: 3.793
 
DOIhttp://dx.doi.org/10.1002/lt.21094
 
ISI Accession Number IDWOS:000245596400015
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYang, ZF
 
dc.contributor.authorHo, DWY
 
dc.contributor.authorNgai, P
 
dc.contributor.authorLau, CK
 
dc.contributor.authorZhao, Y
 
dc.contributor.authorPoon, RTP
 
dc.contributor.authorFan, ST
 
dc.date.accessioned2010-09-06T08:44:47Z
 
dc.date.available2010-09-06T08:44:47Z
 
dc.date.issued2007
 
dc.description.abstractThe present study aims to investigate the potential therapeutic role of interleukin-10 (IL-10) in small-for-size liver transplantation. A syngenic rat orthotopic liver transplantation model was performed using either whole or 40% liver volume of Lewis rats as grafts according to the experimental design. IL-10 was given to the 40% grafts right after reperfusion, and also at 24 and 48 hours after transplantation. When no treatment was given, less than 40% of the small-for-size grafts survived indefinitely, whereas IL-10 treatment could increase the long-term survival rate of the small-for-size grafts to 80%. The 40% grafts presented with extensive areas of necrosis and increased number of apoptotic cells at the early phases after reperfusion. In addition, upregulation of plasma protein carbonyl content (PCC) levels was also detected in the 40% graft group. IL-10 treatment suppressed the upregulation of allograft inflammatory factor-1 (AIF-1) on macrophages in the 40% grafts, and at the same time, decreased the levels of plasma PCC, and improved the histology and function of the 40% grafts. The expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, and caspase 9 in the 40% grafts were upregulated after reperfusion, whereas the augmentation could be suppressed by the administration of IL-10. Finally, IL-10 culture could block AIF-1-mediated NO production and downregulate the expression of iNOS and TNF-α in a macrophage cell line. In conclusion, IL-10 rescued the small-for-size liver grafts by its antiinflammatory properties, through inhibition of AIF-1 mediated proinflammatory and proapoptotic activities of the macrophages during the early period after ischemia/reperfusion. © 2007 AASLD.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationLiver Transplantation, 2007, v. 13 n. 4, p. 558-565 [How to Cite?]
DOI: http://dx.doi.org/10.1002/lt.21094
 
dc.identifier.doihttp://dx.doi.org/10.1002/lt.21094
 
dc.identifier.epage565
 
dc.identifier.hkuros126589
 
dc.identifier.isiWOS:000245596400015
 
dc.identifier.issn1527-6465
2013 Impact Factor: 3.793
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid17394154
 
dc.identifier.scopuseid_2-s2.0-34247235684
 
dc.identifier.spage558
 
dc.identifier.urihttp://hdl.handle.net/10722/83752
 
dc.identifier.volume13
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021
 
dc.publisher.placeUnited States
 
dc.relation.ispartofLiver Transplantation
 
dc.relation.referencesReferences in Scopus
 
dc.rightsLiver Transplantation. Copyright © John Wiley & Sons, Inc.
 
dc.titleAntiinflammatory properties of IL-10 rescue small-for-size liver grafts
 
dc.typeArticle
 
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<contributor.author>Zhao, Y</contributor.author>
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<contributor.author>Fan, ST</contributor.author>
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<description.abstract>The present study aims to investigate the potential therapeutic role of interleukin-10 (IL-10) in small-for-size liver transplantation. A syngenic rat orthotopic liver transplantation model was performed using either whole or 40% liver volume of Lewis rats as grafts according to the experimental design. IL-10 was given to the 40% grafts right after reperfusion, and also at 24 and 48 hours after transplantation. When no treatment was given, less than 40% of the small-for-size grafts survived indefinitely, whereas IL-10 treatment could increase the long-term survival rate of the small-for-size grafts to 80%. The 40% grafts presented with extensive areas of necrosis and increased number of apoptotic cells at the early phases after reperfusion. In addition, upregulation of plasma protein carbonyl content (PCC) levels was also detected in the 40% graft group. IL-10 treatment suppressed the upregulation of allograft inflammatory factor-1 (AIF-1) on macrophages in the 40% grafts, and at the same time, decreased the levels of plasma PCC, and improved the histology and function of the 40% grafts. The expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-&#945;, and caspase 9 in the 40% grafts were upregulated after reperfusion, whereas the augmentation could be suppressed by the administration of IL-10. Finally, IL-10 culture could block AIF-1-mediated NO production and downregulate the expression of iNOS and TNF-&#945; in a macrophage cell line. In conclusion, IL-10 rescued the small-for-size liver grafts by its antiinflammatory properties, through inhibition of AIF-1 mediated proinflammatory and proapoptotic activities of the macrophages during the early period after ischemia/reperfusion. &#169; 2007 AASLD.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. Faculty of Medicine