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Article: In situ gene transfer into rat auxiliary liver transplant

TitleIn situ gene transfer into rat auxiliary liver transplant
Authors
Issue Date1997
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.transplantjournal.com
Citation
Transplantation, 1997, v. 64 n. 11, p. 1537-1541 How to Cite?
AbstractBackground. A replication-defective retrovirus BAG vector was tested for in situ delivery of the β-galactosidase gene to auxiliary liver transplant in a rat model. Methods. The BAG vector, which was shown to be effective in genetic transduction of cultured NIH/3T3 cells, was produced in a ψ2 packaging cell and later amplified in a selected PA317 clone. Hepatocyte replication was induced by one-third hepatectomy of the donor liver, and the procedure was followed by auxiliary partial liver transplantation. Twenty- four hours after hepatic induction or transplantation, vital supernatant at 37°C was perfused into the liver graft via the portal vein during a temporary occlusion of the graft portal vein. Results. All animals survived the transplantation procedures and were killed at specified time intervals. Histochemical staining of the liver graft specimens indicated the expression of β-galactosidase in the gene transferred group but not in the control animals. As demonstrated by polymerase chain reaction assay, the proviral β- galactosidase sequence was present in the graft specimens, but absent from all other tissues tested. Conclusions. In short, the retrovirus BAG vector can be useful for in situ delivery of foreign genes to liver graft in transplantation and other clinical settings, providing a simple, consistent, and reliable alternative in hepatic gene therapy experiments.
Persistent Identifierhttp://hdl.handle.net/10722/83657
ISSN
2015 Impact Factor: 3.69
2015 SCImago Journal Rankings: 1.699
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, YNen_HK
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorChung, Sen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-06T08:43:38Z-
dc.date.available2010-09-06T08:43:38Z-
dc.date.issued1997en_HK
dc.identifier.citationTransplantation, 1997, v. 64 n. 11, p. 1537-1541en_HK
dc.identifier.issn0041-1337en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83657-
dc.description.abstractBackground. A replication-defective retrovirus BAG vector was tested for in situ delivery of the β-galactosidase gene to auxiliary liver transplant in a rat model. Methods. The BAG vector, which was shown to be effective in genetic transduction of cultured NIH/3T3 cells, was produced in a ψ2 packaging cell and later amplified in a selected PA317 clone. Hepatocyte replication was induced by one-third hepatectomy of the donor liver, and the procedure was followed by auxiliary partial liver transplantation. Twenty- four hours after hepatic induction or transplantation, vital supernatant at 37°C was perfused into the liver graft via the portal vein during a temporary occlusion of the graft portal vein. Results. All animals survived the transplantation procedures and were killed at specified time intervals. Histochemical staining of the liver graft specimens indicated the expression of β-galactosidase in the gene transferred group but not in the control animals. As demonstrated by polymerase chain reaction assay, the proviral β- galactosidase sequence was present in the graft specimens, but absent from all other tissues tested. Conclusions. In short, the retrovirus BAG vector can be useful for in situ delivery of foreign genes to liver graft in transplantation and other clinical settings, providing a simple, consistent, and reliable alternative in hepatic gene therapy experiments.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.transplantjournal.comen_HK
dc.relation.ispartofTransplantationen_HK
dc.rightsTransplantation. Copyright © Lippincott Williams & Wilkins.en_HK
dc.titleIn situ gene transfer into rat auxiliary liver transplanten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0041-1337&volume=64&spage=1537&epage=1541&date=1997&atitle=In+situ+gene+transfer+into+rat+auxiliary+liver+transplanten_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/00007890-199712150-00006en_HK
dc.identifier.pmid9415553-
dc.identifier.scopuseid_2-s2.0-0031442993en_HK
dc.identifier.hkuros36113en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031442993&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume64en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1537en_HK
dc.identifier.epage1541en_HK
dc.identifier.isiWOS:000071034100006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, YN=7601490264en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridChung, S=37042916800en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK

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