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Article: Targeting glycyrrhetinic acid to hepatic stellate cells in treating rat liver fibrosis

TitleTargeting glycyrrhetinic acid to hepatic stellate cells in treating rat liver fibrosis
Authors
Issue Date2005
Citation
Zhonghua Gan Zang Bing Za Zhi = Zhonghua Ganzangbing Zazhi = Chinese Journal Of Hepatology, 2005, v. 13 n. 9, p. 664-667 How to Cite?
AbstractOBJECTIVES: We synthesized M6P26-HSA as a carrier for hepatic stellate cells (HSC) and coupled it with glycyrrhetinic acid (GA) to get a new conjugate GA-HSA-M6P26. Its organ distribution, specific combination with HSC and anti-fibrotic effect on livers were studied. METHODS: The GA-HSA-M6P26 was labeled with 125I and its organ distribution was detected radiologically. Selective combination of GA-HSA-M6P26 was observed with double immunocytochemic staining and collagen staining of the liver preparations was carried out using Sirius red staining method. The effect of the conjugate on mRNA expression of type I procollagen was studied with real-time PCR in vivo. RT-PCR was used for the effect on mRNA expression of alpha-SMA, MMP-9 and TIMP-1. RESULTS: 10 minutes after GA-HSA-M6P26 i.v. injection, 55%+/-5% of it was distributed in the livers. Double immunocytochemic staining showed that most of GA-HSA-M6P26 was taken up by HSC. With GA- HSA- M6P26 treatment, the collagen deposition in the liver decreased significantly compared with GA and M6P26-HSA treated rats. Similarly, the mRNA expression of type I procollagen and alpha-SMA dropped significantly. As to MMP-9 and TIMP-1, no significant change was shown. CONCLUSION: GA-HSA-M6P26 was selectively delivered to HSC and it showed a significant anti-fibrotic effect on rat liver fibrosis.
Persistent Identifierhttp://hdl.handle.net/10722/83633
ISSN
2015 SCImago Journal Rankings: 0.140

 

DC FieldValueLanguage
dc.contributor.authorZhang, QSen_HK
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorTian, GYen_HK
dc.date.accessioned2010-09-06T08:43:21Z-
dc.date.available2010-09-06T08:43:21Z-
dc.date.issued2005en_HK
dc.identifier.citationZhonghua Gan Zang Bing Za Zhi = Zhonghua Ganzangbing Zazhi = Chinese Journal Of Hepatology, 2005, v. 13 n. 9, p. 664-667en_HK
dc.identifier.issn1007-3418en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83633-
dc.description.abstractOBJECTIVES: We synthesized M6P26-HSA as a carrier for hepatic stellate cells (HSC) and coupled it with glycyrrhetinic acid (GA) to get a new conjugate GA-HSA-M6P26. Its organ distribution, specific combination with HSC and anti-fibrotic effect on livers were studied. METHODS: The GA-HSA-M6P26 was labeled with 125I and its organ distribution was detected radiologically. Selective combination of GA-HSA-M6P26 was observed with double immunocytochemic staining and collagen staining of the liver preparations was carried out using Sirius red staining method. The effect of the conjugate on mRNA expression of type I procollagen was studied with real-time PCR in vivo. RT-PCR was used for the effect on mRNA expression of alpha-SMA, MMP-9 and TIMP-1. RESULTS: 10 minutes after GA-HSA-M6P26 i.v. injection, 55%+/-5% of it was distributed in the livers. Double immunocytochemic staining showed that most of GA-HSA-M6P26 was taken up by HSC. With GA- HSA- M6P26 treatment, the collagen deposition in the liver decreased significantly compared with GA and M6P26-HSA treated rats. Similarly, the mRNA expression of type I procollagen and alpha-SMA dropped significantly. As to MMP-9 and TIMP-1, no significant change was shown. CONCLUSION: GA-HSA-M6P26 was selectively delivered to HSC and it showed a significant anti-fibrotic effect on rat liver fibrosis.en_HK
dc.languageengen_HK
dc.relation.ispartofZhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatologyen_HK
dc.titleTargeting glycyrrhetinic acid to hepatic stellate cells in treating rat liver fibrosisen_HK
dc.typeArticleen_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid16174454-
dc.identifier.scopuseid_2-s2.0-34548070583en_HK
dc.identifier.hkuros118797en_HK
dc.identifier.volume13en_HK
dc.identifier.issue9en_HK
dc.identifier.spage664en_HK
dc.identifier.epage667en_HK
dc.identifier.scopusauthoridZhang, QS=7406720236en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridZhang, J=34868929000en_HK
dc.identifier.scopusauthoridTian, GY=7202950763en_HK

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