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Article: Inhibition of tumor growth and metastasis in vitro and in vivo by targeting macrophage migration inhibitory factor in human neuroblastoma

TitleInhibition of tumor growth and metastasis in vitro and in vivo by targeting macrophage migration inhibitory factor in human neuroblastoma
Authors
Issue Date2006
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2006, v. 25 n. 25, p. 3501-3508 How to Cite?
AbstractMacrophage Migration Inhibitory Factor (Mif) Has Been Defined As A Novel Oncogene. Our Previous Results Have Shown That Mif May Contribute To The Progression Of Neuroblastoma By (A) Inducing N-Myc Expression And (B) Upregulating The Expression Of Angiogenic Factors. The Aim Of This Study Was To Test Whether Tumor Growth Could Be Inhibited By Reduction Of Endogenous Mif Expression In Neuroblastoma And Clarify The Molecular Mechanisms Underlying Mif Reduction On The Control Of Neuroblastoma Growth. We Established Human Neuroblastoma Cell Lines Stably Expressing Antisense Mif (As-Mif) Cdna. These Stable Transfectants Were Characterized By Cell Proliferation, Gene Expression Profile, Tumorigenicity And Metastasis In Vitro And In Vivo. Decreased Mif Expression Was Observed After Transfection With As-Mif In Neuroblastoma Cells And Downregulation Of Mif Expression Significantly Correlated With Decreased Expression Of N-Myc, Ras, C-Met And Trkb At Protein Level. Affymetrix Microarray Analysis Revealed That Expression Of Il-8 And C-Met Was Inhibited And Neuroblastoma-Favorable Genes Such As Ephb6 And Blu Were Upregulated In Mif Reduced Cells. Neuroblastoma Cell Growth Exhibited A Nearly 80% Reduction In As-Mif Transfectants In Vitro. Furthermore, Mice In Which Tumors Formed After Subcutaneous Injection Of As-Mif Transfectants Showed A 90% Reduction In Tumor Growth Compared To Control. Metastasis In Mice Was Also Suppressed Dramatically. Our Data Demonstrate That Targeting Mif Expression Is A Promising Therapeutic Strategy In Human Neuroblastoma Therapy, And Also Identifies The Mif Target Genes For Further Study. © 2006 Nature Publishing Group All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/83594
ISSN
2015 Impact Factor: 7.932
2015 SCImago Journal Rankings: 4.047
ISI Accession Number ID
References
Errata

 

DC FieldValueLanguage
dc.contributor.authorRen, Yen_HK
dc.contributor.authorChan, HMen_HK
dc.contributor.authorFan, Jen_HK
dc.contributor.authorXie, Yen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorLi, Wen_HK
dc.contributor.authorJiang, Gen_HK
dc.contributor.authorLiu, Qen_HK
dc.contributor.authorMeinhardt, Aen_HK
dc.contributor.authorTam, PKHen_HK
dc.date.accessioned2010-09-06T08:42:53Z-
dc.date.available2010-09-06T08:42:53Z-
dc.date.issued2006en_HK
dc.identifier.citationOncogene, 2006, v. 25 n. 25, p. 3501-3508en_HK
dc.identifier.issn0950-9232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83594-
dc.description.abstractMacrophage Migration Inhibitory Factor (Mif) Has Been Defined As A Novel Oncogene. Our Previous Results Have Shown That Mif May Contribute To The Progression Of Neuroblastoma By (A) Inducing N-Myc Expression And (B) Upregulating The Expression Of Angiogenic Factors. The Aim Of This Study Was To Test Whether Tumor Growth Could Be Inhibited By Reduction Of Endogenous Mif Expression In Neuroblastoma And Clarify The Molecular Mechanisms Underlying Mif Reduction On The Control Of Neuroblastoma Growth. We Established Human Neuroblastoma Cell Lines Stably Expressing Antisense Mif (As-Mif) Cdna. These Stable Transfectants Were Characterized By Cell Proliferation, Gene Expression Profile, Tumorigenicity And Metastasis In Vitro And In Vivo. Decreased Mif Expression Was Observed After Transfection With As-Mif In Neuroblastoma Cells And Downregulation Of Mif Expression Significantly Correlated With Decreased Expression Of N-Myc, Ras, C-Met And Trkb At Protein Level. Affymetrix Microarray Analysis Revealed That Expression Of Il-8 And C-Met Was Inhibited And Neuroblastoma-Favorable Genes Such As Ephb6 And Blu Were Upregulated In Mif Reduced Cells. Neuroblastoma Cell Growth Exhibited A Nearly 80% Reduction In As-Mif Transfectants In Vitro. Furthermore, Mice In Which Tumors Formed After Subcutaneous Injection Of As-Mif Transfectants Showed A 90% Reduction In Tumor Growth Compared To Control. Metastasis In Mice Was Also Suppressed Dramatically. Our Data Demonstrate That Targeting Mif Expression Is A Promising Therapeutic Strategy In Human Neuroblastoma Therapy, And Also Identifies The Mif Target Genes For Further Study. © 2006 Nature Publishing Group All Rights Reserved.en_US
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/oncen_HK
dc.relation.ispartofOncogeneen_HK
dc.titleInhibition of tumor growth and metastasis in vitro and in vivo by targeting macrophage migration inhibitory factor in human neuroblastomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0950-9232&volume=25&issue=25&spage=3501&epage=3508&date=2006&atitle=Inhibition+of+tumor+growth+and+metastasis+in+vitro+and+in+vivo+by+targeting+macrophage+migration+inhibitory+factor+in+human+neuroblastomaen_HK
dc.identifier.emailRen, Y: yren@hkucc.hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.onc.1209395en_US
dc.identifier.pmid16449971-
dc.identifier.scopuseid_2-s2.0-33745146514en_US
dc.identifier.hkuros116369en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33745146514&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume25en_US
dc.identifier.issue25en_US
dc.identifier.spage3501en_US
dc.identifier.epage3508en_US
dc.identifier.eissn1476-5594-
dc.identifier.isiWOS:000238448200002-
dc.relation.erratumdoi:10.1038/sj.onc.1210005-
dc.identifier.citeulike494382-

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