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Article: Large-scale isolation and production of microcarrier-attached porcine hepatocytes for bioartificial livers

TitleLarge-scale isolation and production of microcarrier-attached porcine hepatocytes for bioartificial livers
Authors
Issue Date1996
PublisherElsevier (Singapore) Pte Ltd, Hong Kong Branch. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/708511/description#description
Citation
Asian Journal Of Surgery, 1996, v. 19 n. 1, p. 50-55 How to Cite?
AbstractThe use of hepatocytes in the construction of bioartificial livers (BALs) is currently being investigated. The loading of microcarrier-attached hepatocytes into hollow fibres is one BAL system that has been successful in clinical trials. A reliable method for the isolation and production of large numbers of metabolically-active microcarrier-attached hepatocytes is necessary for this system. In this study, a large volume of pre-perfusion medium and collagenase P was used for harvesting hepatocytes from porcine liver; a cell yield of 1.16 x 10 10 ± 0.36 x 10 10 was obtained and the viability of hepatocytes was more than 92%. By incubating the hepatocytes in DMEM/F12 medium supplemented with 10% foetal calf serum, insulin (0.5 U/ml) and dexamethasone (7.5 μg/ml) in a microcarrier spinner vessel, 80% hepatocyte attachment to microcarrier was achieved at a cell concentration of 2 x 10 9, with cell viability of more than 85% after 24 hours' incubation. These cells were shown to be metabolically active. The method described here could provide sufficient microcarrier-attached hepatocytes for the construction of a BAL.
Persistent Identifierhttp://hdl.handle.net/10722/83590
ISSN
2015 Impact Factor: 0.912
2015 SCImago Journal Rankings: 0.427

 

DC FieldValueLanguage
dc.contributor.authorHo, DWYen_HK
dc.contributor.authorTo, JYTen_HK
dc.contributor.authorYau, KYen_HK
dc.contributor.authorYu, WCen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-06T08:42:50Z-
dc.date.available2010-09-06T08:42:50Z-
dc.date.issued1996en_HK
dc.identifier.citationAsian Journal Of Surgery, 1996, v. 19 n. 1, p. 50-55en_HK
dc.identifier.issn1015-9584en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83590-
dc.description.abstractThe use of hepatocytes in the construction of bioartificial livers (BALs) is currently being investigated. The loading of microcarrier-attached hepatocytes into hollow fibres is one BAL system that has been successful in clinical trials. A reliable method for the isolation and production of large numbers of metabolically-active microcarrier-attached hepatocytes is necessary for this system. In this study, a large volume of pre-perfusion medium and collagenase P was used for harvesting hepatocytes from porcine liver; a cell yield of 1.16 x 10 10 ± 0.36 x 10 10 was obtained and the viability of hepatocytes was more than 92%. By incubating the hepatocytes in DMEM/F12 medium supplemented with 10% foetal calf serum, insulin (0.5 U/ml) and dexamethasone (7.5 μg/ml) in a microcarrier spinner vessel, 80% hepatocyte attachment to microcarrier was achieved at a cell concentration of 2 x 10 9, with cell viability of more than 85% after 24 hours' incubation. These cells were shown to be metabolically active. The method described here could provide sufficient microcarrier-attached hepatocytes for the construction of a BAL.en_HK
dc.languageengen_HK
dc.publisherElsevier (Singapore) Pte Ltd, Hong Kong Branch. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/708511/description#descriptionen_HK
dc.relation.ispartofAsian Journal of Surgeryen_HK
dc.titleLarge-scale isolation and production of microcarrier-attached porcine hepatocytes for bioartificial liversen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1015-9584&volume=19&issue=1&spage=50&epage=55&date=1996&atitle=Large-scale+isolation+and+production+of+microcarrier-attached+porcine+hepatocytes+for+bioartificial+liversen_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0030070916en_HK
dc.identifier.hkuros10984en_HK
dc.identifier.volume19en_HK
dc.identifier.issue1en_HK
dc.identifier.spage50en_HK
dc.identifier.epage55en_HK
dc.publisher.placeHong Kongen_HK
dc.identifier.scopusauthoridHo, DWY=7402971906en_HK
dc.identifier.scopusauthoridTo, JYT=6603618643en_HK
dc.identifier.scopusauthoridYau, KY=7101940492en_HK
dc.identifier.scopusauthoridYu, WC=24490445800en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK

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