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Article: Role of nitric oxide in intestinal ischaemia-reperfusion injury studied using electron paramagnetic resonance

TitleRole of nitric oxide in intestinal ischaemia-reperfusion injury studied using electron paramagnetic resonance
Authors
Issue Date1999
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.bjs.co.uk
Citation
British Journal Of Surgery, 1999, v. 86 n. 11, p. 1427-1432 How to Cite?
AbstractBackground: The Role Of Nitric Oxide In Intestinal Ischaemia-Reperfusion (I/R) Remains Poorly Defined, Partly Because Of Difficulty In Detecting The Nitric Oxide Free Radical. In This Study Nitric Oxide Production Was Assessed During Intestinal I/R By Direct Measurement Using Electron Paramagnetic Resonance (Epr), And The Production Of Nitric Oxide In Jejunum And Ileum Was Correlated With Their Different Abilities To Resist I/R Injury. Methods: Rats Were Given An Electron Spin Trapper (Diethyldithiocarbamate/Ferrous Citrate) By Intraperitoneal Injection. Thirty-Six Segments Each Of Jejunum And Ileum Were Subjected To 15-90 Min Of Ischaemia And 25 Min Of Reperfusion. Tissue Samples Were Analysed For Epr Signals Using A Spectrometer. Results: Mean (S.D.) Basal Nitric Oxide Level Was Significantly Higher In Ileum (3.39 (1.42) Units) Than Jejunum (0.65 (0.05) Units) (P = 0.0005). Increasing Ischaemic Times In The Ileum Resulted In Decreasing Nitric Oxide Levels (85, 32 And 13 Per Cent Of Basal Level At 30, 60 And 90 Min Respectively); Reperfusion Resulted In Further Nitric Oxide Reduction (Mean Decrease 26 Per Cent). Severe (Grade 3) Histological Damage Was Observed In Low Nitric Oxide States (After 15 Min Of I/R In Jejunum, 60 Min Of I/R In Ileum). Conclusion: Nitric Oxide Can Be Measured In Intestinal Tissues Directly By Epr. The Findings Support A Protective Role For Nitric Oxide In I/R, And Offer An Explanation For The Greater Resistance To I/R Of Ileum.
Persistent Identifierhttp://hdl.handle.net/10722/83506
ISSN
2015 Impact Factor: 5.596
2015 SCImago Journal Rankings: 2.966
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, KLen_HK
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorFung, PCWen_HK
dc.contributor.authorGuo, WHen_HK
dc.contributor.authorTam, PKHen_HK
dc.date.accessioned2010-09-06T08:41:51Z-
dc.date.available2010-09-06T08:41:51Z-
dc.date.issued1999en_HK
dc.identifier.citationBritish Journal Of Surgery, 1999, v. 86 n. 11, p. 1427-1432en_US
dc.identifier.issn0007-1323en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83506-
dc.description.abstractBackground: The Role Of Nitric Oxide In Intestinal Ischaemia-Reperfusion (I/R) Remains Poorly Defined, Partly Because Of Difficulty In Detecting The Nitric Oxide Free Radical. In This Study Nitric Oxide Production Was Assessed During Intestinal I/R By Direct Measurement Using Electron Paramagnetic Resonance (Epr), And The Production Of Nitric Oxide In Jejunum And Ileum Was Correlated With Their Different Abilities To Resist I/R Injury. Methods: Rats Were Given An Electron Spin Trapper (Diethyldithiocarbamate/Ferrous Citrate) By Intraperitoneal Injection. Thirty-Six Segments Each Of Jejunum And Ileum Were Subjected To 15-90 Min Of Ischaemia And 25 Min Of Reperfusion. Tissue Samples Were Analysed For Epr Signals Using A Spectrometer. Results: Mean (S.D.) Basal Nitric Oxide Level Was Significantly Higher In Ileum (3.39 (1.42) Units) Than Jejunum (0.65 (0.05) Units) (P = 0.0005). Increasing Ischaemic Times In The Ileum Resulted In Decreasing Nitric Oxide Levels (85, 32 And 13 Per Cent Of Basal Level At 30, 60 And 90 Min Respectively); Reperfusion Resulted In Further Nitric Oxide Reduction (Mean Decrease 26 Per Cent). Severe (Grade 3) Histological Damage Was Observed In Low Nitric Oxide States (After 15 Min Of I/R In Jejunum, 60 Min Of I/R In Ileum). Conclusion: Nitric Oxide Can Be Measured In Intestinal Tissues Directly By Epr. The Findings Support A Protective Role For Nitric Oxide In I/R, And Offer An Explanation For The Greater Resistance To I/R Of Ileum.en_US
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.bjs.co.uken_HK
dc.relation.ispartofBritish Journal of Surgeryen_HK
dc.rightsBritish Journal of Surgery. Copyright © John Wiley & Sons Ltd.en_HK
dc.titleRole of nitric oxide in intestinal ischaemia-reperfusion injury studied using electron paramagnetic resonanceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1323&volume=86&spage=1427&epage=1432&date=1999&atitle=Role+of+nitric+oxide+in+intestinal+ischaemia-reperfusion+injury+studied+using+electron+paramagnetic+resonanceen_HK
dc.identifier.emailChan, KL: klchan@HKUCC.hku.hken_HK
dc.identifier.emailZhang, X: ynzhangx@sohu.comen_HK
dc.identifier.emailFung, PCW: hrspfcw@hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1046/j.1365-2168.1999.01241.xen_US
dc.identifier.pmid10583290-
dc.identifier.scopuseid_2-s2.0-0032695070en_US
dc.identifier.hkuros47290en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032695070&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume86en_US
dc.identifier.issue11en_US
dc.identifier.spage1427en_US
dc.identifier.epage1432en_US
dc.identifier.isiWOS:000083589900013-

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