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Article: The immune reponse following small bowel transplantation: an unusual pattern of cytokine expression

TitleThe immune reponse following small bowel transplantation: an unusual pattern of cytokine expression
Authors
Issue Date1996
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.transplantjournal.com
Citation
Transplantation, 1996, v. 62 n. 6, p. 851-855 How to Cite?
AbstractAcute cell mediated graft rejection is frequently associated with an immune response dominated by cytokines like IL-2 and IFNγ. While small bowel grafts are rejected acutely, there is little information on the type of immune response generated following transplantation and, in particular, whether the cytokine profile resembles that seen during the rejection of other solid organ grafts. In this paper we compare the expression of cytokines in isolated gut tissue following experimental small bowel transplantation with that in heart grafts. Heterotopic small bowel (n=32) and cardiac (n=32) transplants were performed using the following rat strain combinations: syngeneic Lewis (Lew) > Lew (n=8), blood group D Agouti (DA) > DA (n=8) and allogeneic Lew > DA (n=8), DA > Lew (n=8). Two rats from each group were sacrificed at 1, 3, 5, or 7 days after transplantation. RNA was prepared separately from gut wall, after removing the Peyer's patches (PPs) and mesenteric lymph nodes (MLNs) and from heart. Cytokine (IL-1α, IL-2, IL- 4, IL-6, IL-10 and IFNγ) transcripts were analyzed using semiquantitative RT-PCR. Most notably, transcripts of only a single cytokine, IFNγ, became progressively elevated with time in the rejecting small bowel grafts. This is in marked contrast to the findings presented here for rat cardiac grafts in which transcripts of all cytokines tested show an increase with rejection. This significant and steady increase in IFNγ expression occurred before there was any clinical or histological evidence of rejection. These data demonstrate that the mechanisms of rejection in small bowel and other solid organ grafts are likely to be different. Further, the unique rise in IFNγ expression in the gut wall may be a valuable and early indicator of graft rejection.
Persistent Identifierhttp://hdl.handle.net/10722/83457
ISSN
2015 Impact Factor: 3.69
2015 SCImago Journal Rankings: 1.699
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorToogood, GJen_HK
dc.contributor.authorRankin, AMen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorMorris, PJMen_HK
dc.contributor.authorDallman, MJen_HK
dc.date.accessioned2010-09-06T08:41:16Z-
dc.date.available2010-09-06T08:41:16Z-
dc.date.issued1996en_HK
dc.identifier.citationTransplantation, 1996, v. 62 n. 6, p. 851-855en_HK
dc.identifier.issn0041-1337en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83457-
dc.description.abstractAcute cell mediated graft rejection is frequently associated with an immune response dominated by cytokines like IL-2 and IFNγ. While small bowel grafts are rejected acutely, there is little information on the type of immune response generated following transplantation and, in particular, whether the cytokine profile resembles that seen during the rejection of other solid organ grafts. In this paper we compare the expression of cytokines in isolated gut tissue following experimental small bowel transplantation with that in heart grafts. Heterotopic small bowel (n=32) and cardiac (n=32) transplants were performed using the following rat strain combinations: syngeneic Lewis (Lew) > Lew (n=8), blood group D Agouti (DA) > DA (n=8) and allogeneic Lew > DA (n=8), DA > Lew (n=8). Two rats from each group were sacrificed at 1, 3, 5, or 7 days after transplantation. RNA was prepared separately from gut wall, after removing the Peyer's patches (PPs) and mesenteric lymph nodes (MLNs) and from heart. Cytokine (IL-1α, IL-2, IL- 4, IL-6, IL-10 and IFNγ) transcripts were analyzed using semiquantitative RT-PCR. Most notably, transcripts of only a single cytokine, IFNγ, became progressively elevated with time in the rejecting small bowel grafts. This is in marked contrast to the findings presented here for rat cardiac grafts in which transcripts of all cytokines tested show an increase with rejection. This significant and steady increase in IFNγ expression occurred before there was any clinical or histological evidence of rejection. These data demonstrate that the mechanisms of rejection in small bowel and other solid organ grafts are likely to be different. Further, the unique rise in IFNγ expression in the gut wall may be a valuable and early indicator of graft rejection.-
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.transplantjournal.comen_HK
dc.relation.ispartofTransplantationen_HK
dc.rightsTransplantation. Copyright © Lippincott Williams & Wilkins.en_HK
dc.titleThe immune reponse following small bowel transplantation: an unusual pattern of cytokine expressionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0041-1337&volume=62&spage=851&epage=855&date=1996&atitle=The+immune+reponse+following+small+bowel+transplantation:+an+unusual+pattern+of+cytokine+expressionen_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/00007890-199609270-00025-
dc.identifier.pmid8824488-
dc.identifier.scopuseid_2-s2.0-0029778845-
dc.identifier.hkuros23463en_HK
dc.identifier.isiWOS:A1996VK61100025-

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