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Article: Circulating lamin B1 (LMNB1) biomarker detects early stages of liver cancer in patients

TitleCirculating lamin B1 (LMNB1) biomarker detects early stages of liver cancer in patients
Authors
KeywordsCirculating mRNA
Early HCC
Proteomic profiling
Tumor marker
Issue Date2010
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jprobs
Citation
Journal Of Proteome Research, 2010, v. 9 n. 1, p. 70-78 How to Cite?
AbstractHepatocellular carcinoma (HCC) is a major liver malignancy possessing a high mortality rate and is particularly prevalent in China and Asia. While surgery is the most effective treatment for liver tumor, about 80% of HCC patients are inoperable at presentation and die early due to late diagnosis. For early cancer detection, we employed a proteomic expression profiling approach to identify biomarkers for early stages of HCC and subsequently assessed the clinical feasibility of a novel marker in plasma. Frozen liver tissues from a retrospective cohort of 75 liver patients (39 HCCs, 20 cirrhosis, and 16 nondiseased subjects) were subjected to proteome-wide expression profiling by 2-DE. MALDI-TOF/TOF was used to identify differentially expressed proteins, which were further confirmed by immunoblotting, qPCR, and immunohistochemistry. Conventional RT-PCR was employed to further analyze the abundance of selected biomarker at mRNA level in a separate cohort of 63 plasma samples (35 HCCs, 16 liver cirrhosis, 12 healthy individuals). We successfully identified lamin B1 (LMNB1) that was significantly upregulated in HCC tumors and present in patients' plasma. LMNB1 functions in nuclear envelope lamina and possesses a transcriptional coregulatory activity having an important role in DNA replication, cellular aging, and stress responses. Clinically, the expression level of lamin B1 correlated positively with tumor stages, tumor sizes, and number of nodules. Our findings further showed elevation of circulating LMNB1 marker in plasma could detect early stages of HCC patients, with 76% sensitivity and 82% specificity. In conclusion, lamin B1 is a clinically useful biomarker for early stages of HCC in tumor tissues and plasma, and warrants further clinical investigation. © 2010 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/83454
ISSN
2015 Impact Factor: 4.173
2015 SCImago Journal Rankings: 1.962
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of Hong KongN_HKU718/03
Sun C.Y. Research Foundation for Hepatobiliary and Pancreatic Surgery
Funding Information:

we thank the technical Support from the Surgical Tissue Bank staff (Jasmine Yu, S. Y. Yik, Noel Kwong) and insightful advice and comments by Professor S. T. Fan (Queen Mary Hospital, HK). The work was supported by the Research Grants Council of Hong Kong (N_HKU718/03) and Sun C.Y. Research Foundation for Hepatobiliary and Pancreatic Surgery.

References

 

DC FieldValueLanguage
dc.contributor.authorSun, Sen_HK
dc.contributor.authorXu, MZen_HK
dc.contributor.authorPoon, RTen_HK
dc.contributor.authorDay, PJen_HK
dc.contributor.authorLuk, JMen_HK
dc.date.accessioned2010-09-06T08:41:14Z-
dc.date.available2010-09-06T08:41:14Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Proteome Research, 2010, v. 9 n. 1, p. 70-78en_HK
dc.identifier.issn1535-3893en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83454-
dc.description.abstractHepatocellular carcinoma (HCC) is a major liver malignancy possessing a high mortality rate and is particularly prevalent in China and Asia. While surgery is the most effective treatment for liver tumor, about 80% of HCC patients are inoperable at presentation and die early due to late diagnosis. For early cancer detection, we employed a proteomic expression profiling approach to identify biomarkers for early stages of HCC and subsequently assessed the clinical feasibility of a novel marker in plasma. Frozen liver tissues from a retrospective cohort of 75 liver patients (39 HCCs, 20 cirrhosis, and 16 nondiseased subjects) were subjected to proteome-wide expression profiling by 2-DE. MALDI-TOF/TOF was used to identify differentially expressed proteins, which were further confirmed by immunoblotting, qPCR, and immunohistochemistry. Conventional RT-PCR was employed to further analyze the abundance of selected biomarker at mRNA level in a separate cohort of 63 plasma samples (35 HCCs, 16 liver cirrhosis, 12 healthy individuals). We successfully identified lamin B1 (LMNB1) that was significantly upregulated in HCC tumors and present in patients' plasma. LMNB1 functions in nuclear envelope lamina and possesses a transcriptional coregulatory activity having an important role in DNA replication, cellular aging, and stress responses. Clinically, the expression level of lamin B1 correlated positively with tumor stages, tumor sizes, and number of nodules. Our findings further showed elevation of circulating LMNB1 marker in plasma could detect early stages of HCC patients, with 76% sensitivity and 82% specificity. In conclusion, lamin B1 is a clinically useful biomarker for early stages of HCC in tumor tissues and plasma, and warrants further clinical investigation. © 2010 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jprobsen_HK
dc.relation.ispartofJournal of Proteome Researchen_HK
dc.subjectCirculating mRNAen_HK
dc.subjectEarly HCCen_HK
dc.subjectProteomic profilingen_HK
dc.subjectTumor markeren_HK
dc.subject.meshLamin Type B - blood - genetics-
dc.subject.meshLiver Neoplasms - blood - genetics-
dc.subject.meshRNA, Messenger - blood - genetics-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshTumor Markers, Biological - blood - genetics-
dc.titleCirculating lamin B1 (LMNB1) biomarker detects early stages of liver cancer in patientsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1535-3893&volume=9&issue=1&spage=70&epage=78&date=2010&atitle=Circulating+Lamin+B1+(LMNB1)+biomarker+detects+early+stages+of+liver+cancer+in+patientsen_HK
dc.identifier.emailPoon, RT: poontp@hkucc.hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.authorityPoon, RT=rp00446en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/pr9002118en_HK
dc.identifier.pmid19522540-
dc.identifier.scopuseid_2-s2.0-73649104101en_HK
dc.identifier.hkuros168583en_HK
dc.identifier.hkuros206156-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73649104101&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue1en_HK
dc.identifier.spage70en_HK
dc.identifier.epage78en_HK
dc.identifier.isiWOS:000273267900008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSun, S=21740136100en_HK
dc.identifier.scopusauthoridXu, MZ=24339881700en_HK
dc.identifier.scopusauthoridPoon, RT=7103097223en_HK
dc.identifier.scopusauthoridDay, PJ=7202148832en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK

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