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Article: Array-based comparative genomic hybridization reveals recurrent chromosomal aberrations and Jab1 as a potential target for 8q gain in hepatocellular carcinoma

TitleArray-based comparative genomic hybridization reveals recurrent chromosomal aberrations and Jab1 as a potential target for 8q gain in hepatocellular carcinoma
Authors
Issue Date2005
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
Citation
Carcinogenesis, 2005, v. 26 n. 12, p. 2050-2057 How to Cite?
Abstract
Hepatocellular carcinoma (HCC) is one of the major malignancies worldwide. We have previously characterized global gene expression patterns in HCC using microarrays. Here, we report the analysis of genomic DNA copy number among 49 HCC samples using BAC array-based comparative genomic hybridization (CGH). We observed recurrent and characteristic chromosomal aberrations, including frequent DNA copy number gains of 1q, 6p, 8q and 20q, and losses of 4q, 8p, 13q, 16q and 17p. We correlated gene expression with array CGH data, and identified a set of genes whose expression levels correlated with common chromosomal aberrations in HCC. Especially, we noticed that high expression of Jab1 in HCC significantly correlated with DNA copy number gain at 8q. Quantitative microsatellite analysis further confirmed DNA copy number gain at the Jab1 locus. Overexpression of Jab1 in HCC was also validated using real-time RT-PCR, and Jab1 protein levels were studied by immunohistochemistry on tissue microarrays. Functional analysis in HCC cell lines demonstrated that Jab1 may regulate HCC cell proliferation, thereby having a potential role in HCC development. In conclusion, this study shows that array-based CGH provides high resolution mapping of chromosomal aberrations in HCC, and demonstrates the feasibility of correlating array CGH data with gene expression data to identify novel oncogenes and tumor suppressor genes. © Oxford University Press 2005; all rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/83395
ISSN
2013 Impact Factor: 5.266
ISI Accession Number ID
References

 

Author Affiliations
  1. University of California, San Francisco
  2. Universität Bonn
  3. Van Andel Research Institute
  4. Stanford University
  5. The University of Hong Kong
  6. Peking University
DC FieldValueLanguage
dc.contributor.authorPatil, MAen_HK
dc.contributor.authorGütgemann, Ien_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorHo, Cen_HK
dc.contributor.authorCheung, STen_HK
dc.contributor.authorGinzinger, Den_HK
dc.contributor.authorLi, Ren_HK
dc.contributor.authorDykema, KJen_HK
dc.contributor.authorSo, Sen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorKakar, Sen_HK
dc.contributor.authorFurge, KAen_HK
dc.contributor.authorBüttner, Ren_HK
dc.contributor.authorChen, Xen_HK
dc.date.accessioned2010-09-06T08:40:31Z-
dc.date.available2010-09-06T08:40:31Z-
dc.date.issued2005en_HK
dc.identifier.citationCarcinogenesis, 2005, v. 26 n. 12, p. 2050-2057en_HK
dc.identifier.issn0143-3334en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83395-
dc.description.abstractHepatocellular carcinoma (HCC) is one of the major malignancies worldwide. We have previously characterized global gene expression patterns in HCC using microarrays. Here, we report the analysis of genomic DNA copy number among 49 HCC samples using BAC array-based comparative genomic hybridization (CGH). We observed recurrent and characteristic chromosomal aberrations, including frequent DNA copy number gains of 1q, 6p, 8q and 20q, and losses of 4q, 8p, 13q, 16q and 17p. We correlated gene expression with array CGH data, and identified a set of genes whose expression levels correlated with common chromosomal aberrations in HCC. Especially, we noticed that high expression of Jab1 in HCC significantly correlated with DNA copy number gain at 8q. Quantitative microsatellite analysis further confirmed DNA copy number gain at the Jab1 locus. Overexpression of Jab1 in HCC was also validated using real-time RT-PCR, and Jab1 protein levels were studied by immunohistochemistry on tissue microarrays. Functional analysis in HCC cell lines demonstrated that Jab1 may regulate HCC cell proliferation, thereby having a potential role in HCC development. In conclusion, this study shows that array-based CGH provides high resolution mapping of chromosomal aberrations in HCC, and demonstrates the feasibility of correlating array CGH data with gene expression data to identify novel oncogenes and tumor suppressor genes. © Oxford University Press 2005; all rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/en_HK
dc.relation.ispartofCarcinogenesisen_HK
dc.rightsCarcinogenesis. Copyright © Oxford University Press.en_HK
dc.titleArray-based comparative genomic hybridization reveals recurrent chromosomal aberrations and Jab1 as a potential target for 8q gain in hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0143-3334&volume=26&issue=12&spage=2050&epage=2057&date=2005&atitle=Array-based+comparative+genomic+hybridisation+reveals+recurrent+chromosomal+aberrations+and+Jab1+as+a+potential+target+for+8q+gain+in+hepatocellular+carcinomaen_HK
dc.identifier.emailCheung, ST: stcheung@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityCheung, ST=rp00457en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/carcin/bgi178en_HK
dc.identifier.pmid16000397en_HK
dc.identifier.scopuseid_2-s2.0-27944491603en_HK
dc.identifier.hkuros116907en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27944491603&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2050en_HK
dc.identifier.epage2057en_HK
dc.identifier.isiWOS:000233415600003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridPatil, MA=8559878100en_HK
dc.identifier.scopusauthoridGütgemann, I=14825005400en_HK
dc.identifier.scopusauthoridZhang, J=7601356154en_HK
dc.identifier.scopusauthoridHo, C=8914892800en_HK
dc.identifier.scopusauthoridCheung, ST=7202473497en_HK
dc.identifier.scopusauthoridGinzinger, D=7004932822en_HK
dc.identifier.scopusauthoridLi, R=7404723086en_HK
dc.identifier.scopusauthoridDykema, KJ=9334383400en_HK
dc.identifier.scopusauthoridSo, S=7102397384en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridKakar, S=7005050141en_HK
dc.identifier.scopusauthoridFurge, KA=6603630823en_HK
dc.identifier.scopusauthoridBüttner, R=7005353115en_HK
dc.identifier.scopusauthoridChen, X=8978110800en_HK
dc.identifier.citeulike399067-

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