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Article: Identification of local and circulating cancer stem cells in human liver cancer

TitleIdentification of local and circulating cancer stem cells in human liver cancer
Authors
Issue Date2008
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
Citation
Hepatology, 2008, v. 47 n. 3, p. 919-928 How to Cite?
Abstract
Increasing evidence has revealed the importance of cancer stem cells (CSCs) in carcinogenesis. Although liver CSCs have been identified in hepatocellular carcinoma (HCC) cell lines, no data have shown the presence of these cells in human settings. The present study was designed to delineate CSCs serially from HCC cell lines, human liver cancer specimens to blood samples, using CD90 as a potential marker. The number of CD90+ cells increased with the tumorigenicity of HCC cell lines. CD45-CD90+ cells were detected in all the tumor specimens, but not in the normal, cirrhotic, and parallel nontumorous livers. In addition, CD45-CD90+ cells were detectable in 90% of blood samples from liver cancer patients, but none in normal subjects or patients with cirrhosis. A significant positive correlation between the number of CD45-CD90+ cells in the tumor tissues and the number of CD45-CD90+ cells in the blood samples was identified. CD90+ cells sorted from cell lines and CD45-CD90+ cells from the tumor tissues and blood samples of liver cancer patients generated tumor nodules in immunodeficient mice. Serial transplantation of CD90+ cells from tumor xenografts generated tumor nodules in a second and subsequently third batch of immunodeficient mice. Treatment of CD90+ CSCs with anti-human CD44 antibody induced cell apoptosis in a dose-dependent manner. Conclusion: Identification of CD45 -CD90+ CSCs in both tumor tissues and circulation suggests that CD45-CD90+ could be used as a marker for human liver cancer and as a target for the diagnosis and therapy of this malignancy. Copyright © 2007 by the American Association for the Study of Liver Diseases.
Persistent Identifierhttp://hdl.handle.net/10722/83328
ISSN
2013 Impact Factor: 11.190
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorZhen, FYen_HK
dc.contributor.authorNgai, Pen_HK
dc.contributor.authorHo, DWen_HK
dc.contributor.authorWan, CYen_HK
dc.contributor.authorNg, MNPen_HK
dc.contributor.authorChi, KLen_HK
dc.contributor.authorLi, MLYen_HK
dc.contributor.authorKa, HTen_HK
dc.contributor.authorChi, TLen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorSheung, TFen_HK
dc.date.accessioned2010-09-06T08:39:43Z-
dc.date.available2010-09-06T08:39:43Z-
dc.date.issued2008en_HK
dc.identifier.citationHepatology, 2008, v. 47 n. 3, p. 919-928en_HK
dc.identifier.issn0270-9139en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83328-
dc.description.abstractIncreasing evidence has revealed the importance of cancer stem cells (CSCs) in carcinogenesis. Although liver CSCs have been identified in hepatocellular carcinoma (HCC) cell lines, no data have shown the presence of these cells in human settings. The present study was designed to delineate CSCs serially from HCC cell lines, human liver cancer specimens to blood samples, using CD90 as a potential marker. The number of CD90+ cells increased with the tumorigenicity of HCC cell lines. CD45-CD90+ cells were detected in all the tumor specimens, but not in the normal, cirrhotic, and parallel nontumorous livers. In addition, CD45-CD90+ cells were detectable in 90% of blood samples from liver cancer patients, but none in normal subjects or patients with cirrhosis. A significant positive correlation between the number of CD45-CD90+ cells in the tumor tissues and the number of CD45-CD90+ cells in the blood samples was identified. CD90+ cells sorted from cell lines and CD45-CD90+ cells from the tumor tissues and blood samples of liver cancer patients generated tumor nodules in immunodeficient mice. Serial transplantation of CD90+ cells from tumor xenografts generated tumor nodules in a second and subsequently third batch of immunodeficient mice. Treatment of CD90+ CSCs with anti-human CD44 antibody induced cell apoptosis in a dose-dependent manner. Conclusion: Identification of CD45 -CD90+ CSCs in both tumor tissues and circulation suggests that CD45-CD90+ could be used as a marker for human liver cancer and as a target for the diagnosis and therapy of this malignancy. Copyright © 2007 by the American Association for the Study of Liver Diseases.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/en_HK
dc.relation.ispartofHepatologyen_HK
dc.rightsHepatology. Copyright © John Wiley & Sons, Inc.en_HK
dc.titleIdentification of local and circulating cancer stem cells in human liver canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0270-9139&volume=47&issue=3&spage=919&epage=928&date=2008&atitle=Identification+of+local+and+circulating+cancer+stem+cells+in+human+liver+canceren_HK
dc.identifier.emailPoon, RTP: poontp@hkucc.hku.hken_HK
dc.identifier.authorityPoon, RTP=rp00446en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/hep.22082en_HK
dc.identifier.pmid18275073en_HK
dc.identifier.scopuseid_2-s2.0-40949160140en_HK
dc.identifier.hkuros140961en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40949160140&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue3en_HK
dc.identifier.spage919en_HK
dc.identifier.epage928en_HK
dc.identifier.isiWOS:000253698900018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhen, FY=14018809600en_HK
dc.identifier.scopusauthoridNgai, P=23477971900en_HK
dc.identifier.scopusauthoridHo, DW=7402971906en_HK
dc.identifier.scopusauthoridWan, CY=8343494200en_HK
dc.identifier.scopusauthoridNg, MNP=23478329500en_HK
dc.identifier.scopusauthoridChi, KL=15070609500en_HK
dc.identifier.scopusauthoridLi, MLY=27168342600en_HK
dc.identifier.scopusauthoridKa, HT=15070828800en_HK
dc.identifier.scopusauthoridChi, TL=23987591100en_HK
dc.identifier.scopusauthoridPoon, RTP=7103097223en_HK
dc.identifier.scopusauthoridSheung, TF=6506234707en_HK

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