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Article: Role of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft

TitleRole of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft
Authors
Issue Date2001
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurg
Citation
Journal of Pediatric Surgery, 2001, v. 36 n. 2, p. 352-356 How to Cite?
AbstractBackground/Purpose: Results Of Small Bowel Transplantation Remain Unsatisfactory Because Of Severe Immune Rejection. The Current Study Aims To Elucidate The Role Of Activation Of Cd4+ And Cd8+ T Cells In Early And Late Acute Rejection Of Small Bowel Allograft And, Hence, Provide The Immunologic Basis For Developing New Therapeutic Strategies. Methods: We Used An Mhc Fully Mismatched (Da To Lewis) Heterotopic Rat Small Bowel Transplant Model And A Unique Fk506-Based Immunosuppressive Regimen, Which Suppresses Early Acute Rejection But Does Not Prevent Late Acute Rejection. Flow Cytometric Analysis Was Used To Quantitate The Number Of Activated Cd4+ And Cd8+ T Cells In Graft And Host Mesenteric Lymph Nodes. Results: The Survival (Mean ± Sd) Of Intestinal Allograft Was Significantly Prolonged, From 6,6 ± 0.84 Days For The Untreated Group To 40.7 ± 14.1 Days For The Fk506-Treated Group. Activation Of Cd4+ Cells Was Suppressed Significantly In The Fk506-Treated Group On Postoperative Day 7 Compared With The Untreated Group (29.4% ± 3.55% V52.83% ± 11.9%; P < .01). Activation Of Cd8+ Cells Was Similarly Suppressed (31.5 ± 10.34% V 48.53 ± 14.34%; P < .05). Interestingly, At Late Acute Rejection, Activated Cd4+ And Cd8+ T Cells Remained At Almost The Same Low Levels As Those On Postoperative Day 7 In The Fk506-Treated Group. The Spleen To Body Weight Ratio Was Significantly Increased In The Untreated Group (0.53 ± 0.07), And Slightly Increased In The Fk Treated Group (0.27 ± 0.07, On Postoperative Day 7; 0.24 ± 0.07 At Late Acute Rejection) Compared With The Syngeneic Group (0.18 ± 0.02). Conclusion: The Activation Of Cd4+ And Cd8+ T Cells Was Suppressed Effectively By Early Potent Immunosuppressive Treatment Resulting In Prolonged Survival Of Intestinal Allograft. At Late Acute Rejection, The Cd4+ And Cd8+ T Cells Remained At Low-Level Activation Status, In Contrast To The Surge Of Cd4+ And Cd8+ Activation During Early Acute Rejection, This Suggests That Persistent T Cell Activation Even At Low Level Is Sufficient To Cause The Late Acute Rejection Eventually. A Therapeutic Strategy Targeting These Cells Is Needed For Long-Term Engraftment. Copyright © 2001 By W,B. Saunders Company.
Persistent Identifierhttp://hdl.handle.net/10722/83238
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.802
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuo, WHen_HK
dc.contributor.authorTian, Len_HK
dc.contributor.authorChan, KLen_HK
dc.contributor.authorDallman, Men_HK
dc.contributor.authorTam, PKHen_HK
dc.date.accessioned2010-09-06T08:38:39Z-
dc.date.available2010-09-06T08:38:39Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal of Pediatric Surgery, 2001, v. 36 n. 2, p. 352-356en_HK
dc.identifier.issn0022-3468en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83238-
dc.description.abstractBackground/Purpose: Results Of Small Bowel Transplantation Remain Unsatisfactory Because Of Severe Immune Rejection. The Current Study Aims To Elucidate The Role Of Activation Of Cd4+ And Cd8+ T Cells In Early And Late Acute Rejection Of Small Bowel Allograft And, Hence, Provide The Immunologic Basis For Developing New Therapeutic Strategies. Methods: We Used An Mhc Fully Mismatched (Da To Lewis) Heterotopic Rat Small Bowel Transplant Model And A Unique Fk506-Based Immunosuppressive Regimen, Which Suppresses Early Acute Rejection But Does Not Prevent Late Acute Rejection. Flow Cytometric Analysis Was Used To Quantitate The Number Of Activated Cd4+ And Cd8+ T Cells In Graft And Host Mesenteric Lymph Nodes. Results: The Survival (Mean ± Sd) Of Intestinal Allograft Was Significantly Prolonged, From 6,6 ± 0.84 Days For The Untreated Group To 40.7 ± 14.1 Days For The Fk506-Treated Group. Activation Of Cd4+ Cells Was Suppressed Significantly In The Fk506-Treated Group On Postoperative Day 7 Compared With The Untreated Group (29.4% ± 3.55% V52.83% ± 11.9%; P < .01). Activation Of Cd8+ Cells Was Similarly Suppressed (31.5 ± 10.34% V 48.53 ± 14.34%; P < .05). Interestingly, At Late Acute Rejection, Activated Cd4+ And Cd8+ T Cells Remained At Almost The Same Low Levels As Those On Postoperative Day 7 In The Fk506-Treated Group. The Spleen To Body Weight Ratio Was Significantly Increased In The Untreated Group (0.53 ± 0.07), And Slightly Increased In The Fk Treated Group (0.27 ± 0.07, On Postoperative Day 7; 0.24 ± 0.07 At Late Acute Rejection) Compared With The Syngeneic Group (0.18 ± 0.02). Conclusion: The Activation Of Cd4+ And Cd8+ T Cells Was Suppressed Effectively By Early Potent Immunosuppressive Treatment Resulting In Prolonged Survival Of Intestinal Allograft. At Late Acute Rejection, The Cd4+ And Cd8+ T Cells Remained At Low-Level Activation Status, In Contrast To The Surge Of Cd4+ And Cd8+ Activation During Early Acute Rejection, This Suggests That Persistent T Cell Activation Even At Low Level Is Sufficient To Cause The Late Acute Rejection Eventually. A Therapeutic Strategy Targeting These Cells Is Needed For Long-Term Engraftment. Copyright © 2001 By W,B. Saunders Company.en_US
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurgen_HK
dc.relation.ispartofJournal of Pediatric Surgeryen_HK
dc.titleRole of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograften_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3468&volume=36&issue=2&spage=352&epage=356&date=2001&atitle=Role+of+CD4++and+CD8++T+cells+in+early+and+late+acute+rejection+of+small+bowel+allograften_HK
dc.identifier.emailChan, KL: klchan@HKUCC.hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1053/jpsu.2001.20715en_US
dc.identifier.pmid11172433-
dc.identifier.scopuseid_2-s2.0-0035145587en_US
dc.identifier.hkuros58728en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035145587&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume36en_US
dc.identifier.issue2en_US
dc.identifier.spage352en_US
dc.identifier.epage356en_US
dc.identifier.isiWOS:000166697800042-

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