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Article: Role of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft
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TitleRole of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft
 
AuthorsGuo, WH2
Tian, L2
Chan, KL2
Dallman, M2
Tam, PKH2 1
 
Issue Date2001
 
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurg
 
CitationJournal of Pediatric Surgery, 2001, v. 36 n. 2, p. 352-356 [How to Cite?]
DOI: http://dx.doi.org/10.1053/jpsu.2001.20715
 
AbstractBackground/Purpose: Results Of Small Bowel Transplantation Remain Unsatisfactory Because Of Severe Immune Rejection. The Current Study Aims To Elucidate The Role Of Activation Of Cd4+ And Cd8+ T Cells In Early And Late Acute Rejection Of Small Bowel Allograft And, Hence, Provide The Immunologic Basis For Developing New Therapeutic Strategies. Methods: We Used An Mhc Fully Mismatched (Da To Lewis) Heterotopic Rat Small Bowel Transplant Model And A Unique Fk506-Based Immunosuppressive Regimen, Which Suppresses Early Acute Rejection But Does Not Prevent Late Acute Rejection. Flow Cytometric Analysis Was Used To Quantitate The Number Of Activated Cd4+ And Cd8+ T Cells In Graft And Host Mesenteric Lymph Nodes. Results: The Survival (Mean ± Sd) Of Intestinal Allograft Was Significantly Prolonged, From 6,6 ± 0.84 Days For The Untreated Group To 40.7 ± 14.1 Days For The Fk506-Treated Group. Activation Of Cd4+ Cells Was Suppressed Significantly In The Fk506-Treated Group On Postoperative Day 7 Compared With The Untreated Group (29.4% ± 3.55% V52.83% ± 11.9%; P < .01). Activation Of Cd8+ Cells Was Similarly Suppressed (31.5 ± 10.34% V 48.53 ± 14.34%; P < .05). Interestingly, At Late Acute Rejection, Activated Cd4+ And Cd8+ T Cells Remained At Almost The Same Low Levels As Those On Postoperative Day 7 In The Fk506-Treated Group. The Spleen To Body Weight Ratio Was Significantly Increased In The Untreated Group (0.53 ± 0.07), And Slightly Increased In The Fk Treated Group (0.27 ± 0.07, On Postoperative Day 7; 0.24 ± 0.07 At Late Acute Rejection) Compared With The Syngeneic Group (0.18 ± 0.02). Conclusion: The Activation Of Cd4+ And Cd8+ T Cells Was Suppressed Effectively By Early Potent Immunosuppressive Treatment Resulting In Prolonged Survival Of Intestinal Allograft. At Late Acute Rejection, The Cd4+ And Cd8+ T Cells Remained At Low-Level Activation Status, In Contrast To The Surge Of Cd4+ And Cd8+ Activation During Early Acute Rejection, This Suggests That Persistent T Cell Activation Even At Low Level Is Sufficient To Cause The Late Acute Rejection Eventually. A Therapeutic Strategy Targeting These Cells Is Needed For Long-Term Engraftment. Copyright © 2001 By W,B. Saunders Company.
 
ISSN0022-3468
2013 Impact Factor: 1.311
2013 SCImago Journal Rankings: 0.811
 
DOIhttp://dx.doi.org/10.1053/jpsu.2001.20715
 
ISI Accession Number IDWOS:000166697800042
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorGuo, WH
 
dc.contributor.authorTian, L
 
dc.contributor.authorChan, KL
 
dc.contributor.authorDallman, M
 
dc.contributor.authorTam, PKH
 
dc.date.accessioned2010-09-06T08:38:39Z
 
dc.date.available2010-09-06T08:38:39Z
 
dc.date.issued2001
 
dc.description.abstractBackground/Purpose: Results Of Small Bowel Transplantation Remain Unsatisfactory Because Of Severe Immune Rejection. The Current Study Aims To Elucidate The Role Of Activation Of Cd4+ And Cd8+ T Cells In Early And Late Acute Rejection Of Small Bowel Allograft And, Hence, Provide The Immunologic Basis For Developing New Therapeutic Strategies. Methods: We Used An Mhc Fully Mismatched (Da To Lewis) Heterotopic Rat Small Bowel Transplant Model And A Unique Fk506-Based Immunosuppressive Regimen, Which Suppresses Early Acute Rejection But Does Not Prevent Late Acute Rejection. Flow Cytometric Analysis Was Used To Quantitate The Number Of Activated Cd4+ And Cd8+ T Cells In Graft And Host Mesenteric Lymph Nodes. Results: The Survival (Mean ± Sd) Of Intestinal Allograft Was Significantly Prolonged, From 6,6 ± 0.84 Days For The Untreated Group To 40.7 ± 14.1 Days For The Fk506-Treated Group. Activation Of Cd4+ Cells Was Suppressed Significantly In The Fk506-Treated Group On Postoperative Day 7 Compared With The Untreated Group (29.4% ± 3.55% V52.83% ± 11.9%; P < .01). Activation Of Cd8+ Cells Was Similarly Suppressed (31.5 ± 10.34% V 48.53 ± 14.34%; P < .05). Interestingly, At Late Acute Rejection, Activated Cd4+ And Cd8+ T Cells Remained At Almost The Same Low Levels As Those On Postoperative Day 7 In The Fk506-Treated Group. The Spleen To Body Weight Ratio Was Significantly Increased In The Untreated Group (0.53 ± 0.07), And Slightly Increased In The Fk Treated Group (0.27 ± 0.07, On Postoperative Day 7; 0.24 ± 0.07 At Late Acute Rejection) Compared With The Syngeneic Group (0.18 ± 0.02). Conclusion: The Activation Of Cd4+ And Cd8+ T Cells Was Suppressed Effectively By Early Potent Immunosuppressive Treatment Resulting In Prolonged Survival Of Intestinal Allograft. At Late Acute Rejection, The Cd4+ And Cd8+ T Cells Remained At Low-Level Activation Status, In Contrast To The Surge Of Cd4+ And Cd8+ Activation During Early Acute Rejection, This Suggests That Persistent T Cell Activation Even At Low Level Is Sufficient To Cause The Late Acute Rejection Eventually. A Therapeutic Strategy Targeting These Cells Is Needed For Long-Term Engraftment. Copyright © 2001 By W,B. Saunders Company.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal of Pediatric Surgery, 2001, v. 36 n. 2, p. 352-356 [How to Cite?]
DOI: http://dx.doi.org/10.1053/jpsu.2001.20715
 
dc.identifier.doihttp://dx.doi.org/10.1053/jpsu.2001.20715
 
dc.identifier.epage356
 
dc.identifier.hkuros58728
 
dc.identifier.isiWOS:000166697800042
 
dc.identifier.issn0022-3468
2013 Impact Factor: 1.311
2013 SCImago Journal Rankings: 0.811
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmid11172433
 
dc.identifier.scopuseid_2-s2.0-0035145587
 
dc.identifier.spage352
 
dc.identifier.urihttp://hdl.handle.net/10722/83238
 
dc.identifier.volume36
 
dc.languageeng
 
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurg
 
dc.relation.ispartofJournal of Pediatric Surgery
 
dc.relation.referencesReferences in Scopus
 
dc.titleRole of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft
 
dc.typeArticle
 
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<contributor.author>Chan, KL</contributor.author>
<contributor.author>Dallman, M</contributor.author>
<contributor.author>Tam, PKH</contributor.author>
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<description.abstract>Background/Purpose: Results Of Small Bowel Transplantation Remain Unsatisfactory Because Of Severe Immune Rejection. The Current Study Aims To Elucidate The Role Of Activation Of Cd4+ And Cd8+ T Cells In Early And Late Acute Rejection Of Small Bowel Allograft And, Hence, Provide The Immunologic Basis For Developing New Therapeutic Strategies. Methods: We Used An Mhc Fully Mismatched (Da To Lewis) Heterotopic Rat Small Bowel Transplant Model And A Unique Fk506-Based Immunosuppressive Regimen, Which Suppresses Early Acute Rejection But Does Not Prevent Late Acute Rejection. Flow Cytometric Analysis Was Used To Quantitate The Number Of Activated Cd4+ And Cd8+ T Cells In Graft And Host Mesenteric Lymph Nodes. Results: The Survival (Mean &#177; Sd) Of Intestinal Allograft Was Significantly Prolonged, From 6,6 &#177; 0.84 Days For The Untreated Group To 40.7 &#177; 14.1 Days For The Fk506-Treated Group. Activation Of Cd4+ Cells Was Suppressed Significantly In The Fk506-Treated Group On Postoperative Day 7 Compared With The Untreated Group (29.4% &#177; 3.55% V52.83% &#177; 11.9%; P &lt; .01). Activation Of Cd8+ Cells Was Similarly Suppressed (31.5 &#177; 10.34% V 48.53 &#177; 14.34%; P &lt; .05). Interestingly, At Late Acute Rejection, Activated Cd4+ And Cd8+ T Cells Remained At Almost The Same Low Levels As Those On Postoperative Day 7 In The Fk506-Treated Group. The Spleen To Body Weight Ratio Was Significantly Increased In The Untreated Group (0.53 &#177; 0.07), And Slightly Increased In The Fk Treated Group (0.27 &#177; 0.07, On Postoperative Day 7; 0.24 &#177; 0.07 At Late Acute Rejection) Compared With The Syngeneic Group (0.18 &#177; 0.02). Conclusion: The Activation Of Cd4+ And Cd8+ T Cells Was Suppressed Effectively By Early Potent Immunosuppressive Treatment Resulting In Prolonged Survival Of Intestinal Allograft. At Late Acute Rejection, The Cd4+ And Cd8+ T Cells Remained At Low-Level Activation Status, In Contrast To The Surge Of Cd4+ And Cd8+ Activation During Early Acute Rejection, This Suggests That Persistent T Cell Activation Even At Low Level Is Sufficient To Cause The Late Acute Rejection Eventually. A Therapeutic Strategy Targeting These Cells Is Needed For Long-Term Engraftment. Copyright &#169; 2001 By W,B. Saunders Company.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. Imperial College London