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Article: Nasopharyngeal carcinoma cell line (C666-1) consistently harbouring Epstein-Barr virus

TitleNasopharyngeal carcinoma cell line (C666-1) consistently harbouring Epstein-Barr virus
Authors
Issue Date1999
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 1999, v. 83 n. 1, p. 121-126 How to Cite?
AbstractWe have established a cell line (C666-1) from undifferentiated nasopharyngeal carcinoma (NPC). This cell line consistently carries the Epstein-Barr virus (EBV) in long-term cultures. C666-1 is a subclone of its parental cell line, C666, derived from an NPC xenograft of southern Chinese origin. It grows as an adherent culture and lacks contact inhibition. In addition, it is tumorigenic in athymic nude mice. The cells consistently express EBV-encoded RNAs and are positively stained for cytokeratin, an epithelial marker. In addition, they express EBNA1 protein, LMP1 and LMP2 transcripts and thus resemble the EBV latency II pattern. The virus genotype is EBV-1 with the latent membrane protein 1 gene showing a 30-bp deletion at the carboxyl terminus, both consistent with findings in southern Chinese NPC tumours. Cytogenetic analysis revealed a sub-diploid status with a chromosomal modal number of 45. C666-1 is unique among NPC cell lines in that it carries EBV. These cells may serve as a good investigative tool as the viral latency pattern and genotype are observed in the majority of primary NPC biopsies from Chinese patients.
Persistent Identifierhttp://hdl.handle.net/10722/83213
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, STen_HK
dc.contributor.authorHuang, DPen_HK
dc.contributor.authorHui, ABYen_HK
dc.contributor.authorLo, KWen_HK
dc.contributor.authorKo, CWen_HK
dc.contributor.authorTsang, YSen_HK
dc.contributor.authorWong, Nen_HK
dc.contributor.authorWhitney, BMen_HK
dc.contributor.authorLee, JCKen_HK
dc.date.accessioned2010-09-06T08:38:21Z-
dc.date.available2010-09-06T08:38:21Z-
dc.date.issued1999en_HK
dc.identifier.citationInternational Journal Of Cancer, 1999, v. 83 n. 1, p. 121-126en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83213-
dc.description.abstractWe have established a cell line (C666-1) from undifferentiated nasopharyngeal carcinoma (NPC). This cell line consistently carries the Epstein-Barr virus (EBV) in long-term cultures. C666-1 is a subclone of its parental cell line, C666, derived from an NPC xenograft of southern Chinese origin. It grows as an adherent culture and lacks contact inhibition. In addition, it is tumorigenic in athymic nude mice. The cells consistently express EBV-encoded RNAs and are positively stained for cytokeratin, an epithelial marker. In addition, they express EBNA1 protein, LMP1 and LMP2 transcripts and thus resemble the EBV latency II pattern. The virus genotype is EBV-1 with the latent membrane protein 1 gene showing a 30-bp deletion at the carboxyl terminus, both consistent with findings in southern Chinese NPC tumours. Cytogenetic analysis revealed a sub-diploid status with a chromosomal modal number of 45. C666-1 is unique among NPC cell lines in that it carries EBV. These cells may serve as a good investigative tool as the viral latency pattern and genotype are observed in the majority of primary NPC biopsies from Chinese patients.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.titleNasopharyngeal carcinoma cell line (C666-1) consistently harbouring Epstein-Barr virusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=83&spage=121&epage=126&date=1999&atitle=Nasopharyngeal+carcinoma+cell+line+(C666-1)+consistently+harbouring+Epstein-Barr+virusen_HK
dc.identifier.emailCheung, ST: stcheung@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ST=rp00457en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1097-0215(19990924)83:1<121::AID-IJC21>3.0.CO;2-Fen_HK
dc.identifier.pmid10449618-
dc.identifier.scopuseid_2-s2.0-0032822527en_HK
dc.identifier.hkuros51623en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032822527&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume83en_HK
dc.identifier.issue1en_HK
dc.identifier.spage121en_HK
dc.identifier.epage126en_HK
dc.identifier.isiWOS:000082283700021-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCheung, ST=7202473497en_HK
dc.identifier.scopusauthoridHuang, DP=7403891486en_HK
dc.identifier.scopusauthoridHui, ABY=7102453683en_HK
dc.identifier.scopusauthoridLo, KW=7402101603en_HK
dc.identifier.scopusauthoridKo, CW=7202596440en_HK
dc.identifier.scopusauthoridTsang, YS=7007101172en_HK
dc.identifier.scopusauthoridWong, N=7202836653en_HK
dc.identifier.scopusauthoridWhitney, BM=7006822283en_HK
dc.identifier.scopusauthoridLee, JCK=7601456915en_HK

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