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Article: Synergistic Effect of Cool/Thaw Cycles on Vascular Cells in an In Vitro Model of Cryoplasty

TitleSynergistic Effect of Cool/Thaw Cycles on Vascular Cells in an In Vitro Model of Cryoplasty
Authors
Issue Date2008
PublisherElsevier Inc.. The Journal's web site is located at http://www.jvir.org/
Citation
Journal Of Vascular And Interventional Radiology, 2008, v. 19 n. 6, p. 925-930 How to Cite?
AbstractPurpose: Cryoplasty combines mechanical dilation with supercooling of the vessel and has shown encouraging preliminary results in the management of atherosclerotic lesions. However, the mechanisms of action and the optimum inflation regimen are still not well established. This study investigates the effects of single and dual supercooling and rewarming cycles on the survival responses of smooth muscle cells (SMCs) and endothelial cells (ECs). Materials and Methods: Bovine aortic SMCs and ECs were cultured separately in six-well plates with medium supplemented with 10% fetal bovine serum. In the one-cycle treatment group, the cells were supercooled for 60 seconds to -10°C and then rewarmed rapidly in a water bath at 37°C for another 60 seconds. Two-cycle treatment was done by supercooling and rewarming the cells twice. The samples were then put into an incubator at 37°C for 0, 6, 12, and 24 hours. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling was used to measure apoptosis and phospho-Akt immunohistochemistry and immunoblot analysis were employed to assess activation of Akt. Results: A significant increase of apoptotic cells was observed in the two-cycle procedure versus the one-cycle procedure. In both groups, there were more apoptotic SMCs than ECs. Akt activation was higher in ECs by a factor of three compared with SMCs (P < .05). Conclusions: The higher apoptotic rate and the absence of Akt activation of SMCs versus controls in both treatment groups may imply the potential of a lower restenosis rate, especially after two cycles of supercooling and rewarming. Further in vivo and clinical investigations are needed to confirm results of the in vitro testing. © 2008 SIR.
Persistent Identifierhttp://hdl.handle.net/10722/83140
ISSN
2015 Impact Factor: 2.57
2015 SCImago Journal Rankings: 1.210
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYiu, Wken_HK
dc.contributor.authorCheng, SWKen_HK
dc.contributor.authorSumpio, BEen_HK
dc.date.accessioned2010-09-06T08:37:28Z-
dc.date.available2010-09-06T08:37:28Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Vascular And Interventional Radiology, 2008, v. 19 n. 6, p. 925-930en_HK
dc.identifier.issn1051-0443en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83140-
dc.description.abstractPurpose: Cryoplasty combines mechanical dilation with supercooling of the vessel and has shown encouraging preliminary results in the management of atherosclerotic lesions. However, the mechanisms of action and the optimum inflation regimen are still not well established. This study investigates the effects of single and dual supercooling and rewarming cycles on the survival responses of smooth muscle cells (SMCs) and endothelial cells (ECs). Materials and Methods: Bovine aortic SMCs and ECs were cultured separately in six-well plates with medium supplemented with 10% fetal bovine serum. In the one-cycle treatment group, the cells were supercooled for 60 seconds to -10°C and then rewarmed rapidly in a water bath at 37°C for another 60 seconds. Two-cycle treatment was done by supercooling and rewarming the cells twice. The samples were then put into an incubator at 37°C for 0, 6, 12, and 24 hours. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling was used to measure apoptosis and phospho-Akt immunohistochemistry and immunoblot analysis were employed to assess activation of Akt. Results: A significant increase of apoptotic cells was observed in the two-cycle procedure versus the one-cycle procedure. In both groups, there were more apoptotic SMCs than ECs. Akt activation was higher in ECs by a factor of three compared with SMCs (P < .05). Conclusions: The higher apoptotic rate and the absence of Akt activation of SMCs versus controls in both treatment groups may imply the potential of a lower restenosis rate, especially after two cycles of supercooling and rewarming. Further in vivo and clinical investigations are needed to confirm results of the in vitro testing. © 2008 SIR.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc.. The Journal's web site is located at http://www.jvir.org/en_HK
dc.relation.ispartofJournal of Vascular and Interventional Radiologyen_HK
dc.rightsJournal of Vascular and Interventional Radiology. Copyright © Lippincott Williams & Wilkins.en_HK
dc.titleSynergistic Effect of Cool/Thaw Cycles on Vascular Cells in an In Vitro Model of Cryoplastyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1051-0443&volume=19&issue=6&spage=925&epage=930&date=2008&atitle=Synergistic+effect+of+cool/thaw+cycles+on+vascular+cells+in+an+in+vitro+model+of+cryoplastyen_HK
dc.identifier.emailYiu, Wk: waikiyiu@hku.hken_HK
dc.identifier.emailCheng, SWK: wkcheng@hkucc.hku.hken_HK
dc.identifier.authorityYiu, Wk=rp00311en_HK
dc.identifier.authorityCheng, SWK=rp00374en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jvir.2008.02.007en_HK
dc.identifier.pmid18503909-
dc.identifier.scopuseid_2-s2.0-44149128627en_HK
dc.identifier.hkuros151670en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-44149128627&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue6en_HK
dc.identifier.spage925en_HK
dc.identifier.epage930en_HK
dc.identifier.eissn1535-7732-
dc.identifier.isiWOS:000256416300018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYiu, Wk=12763171700en_HK
dc.identifier.scopusauthoridCheng, SWK=7404684779en_HK
dc.identifier.scopusauthoridSumpio, BE=7103201423en_HK

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