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Article: Proteome-based plasma biomarkers for Alzheimer's disease

TitleProteome-based plasma biomarkers for Alzheimer's disease
Authors
KeywordsAlzheimer's disease
Biomarkers
Plasma
Proteomics
Two-dimensional gel electrophoresis (2-DGE)
Issue Date2006
PublisherOxford University Press. The Journal's web site is located at http://brain.oxfordjournals.org/
Citation
Brain, 2006, v. 129 n. 11, p. 3042-3050 How to Cite?
AbstractAlzheimer's disease is a common and devastating disease for which there is no readily available biomarker to aid diagnosis or to monitor disease progression. Biomarkers have been sought in CSF but no previous study has used two-dimensional gel electrophoresis coupled with mass spectrometry to seek biomarkers in peripheral tissue. We performed a case-control study of plasma using this proteomics approach to identify proteins that differ in the disease state relative to aged controls. For discovery-phase proteomics analysis, 50 people with Alzheimer's dementia were recruited through secondary services and 50 normal elderly controls through primary care. For validation purposes a total of 511 subjects with Alzheimer's disease and other neurodegenerative diseases and normal elderly controls were examined. Image analysis of the protein distribution of the gels alone identifies disease cases with 56% sensitivity and 80% specificity. Mass spectrometric analysis of the changes observed in two-dimensional electrophoresis identified a number of proteins previously implicated in the disease pathology, including complement factor H (CFH) precursor and α-2-macroglobulin (α- 2M). Using semi-quantitative immunoblotting, the elevation of CFH and α- 2M was shown to be specific for Alzheimer's disease and to correlate with disease severity although alternative assays would be necessary to improve sensitivity and specificity. These findings suggest that blood may be a rich source for biomarkers of Alzheimer's disease and that CFH, together with other proteins such as α- 2M may be a specific markers of this illness. © 2006 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/81482
ISSN
2015 Impact Factor: 10.103
2015 SCImago Journal Rankings: 6.097
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHye, Aen_HK
dc.contributor.authorLynham, Sen_HK
dc.contributor.authorThambisetty, Men_HK
dc.contributor.authorCausevic, Men_HK
dc.contributor.authorCampbell, Jen_HK
dc.contributor.authorByers, HLen_HK
dc.contributor.authorHooper, Cen_HK
dc.contributor.authorRijsdijk, Fen_HK
dc.contributor.authorTabrizi, SJen_HK
dc.contributor.authorBanner, Sen_HK
dc.contributor.authorShaw, CEen_HK
dc.contributor.authorFoy, Cen_HK
dc.contributor.authorPoppe, Men_HK
dc.contributor.authorArcher, Nen_HK
dc.contributor.authorHamilton, Gen_HK
dc.contributor.authorPowell, Jen_HK
dc.contributor.authorBrown, RGen_HK
dc.contributor.authorSham, Pen_HK
dc.contributor.authorWard, Men_HK
dc.contributor.authorLovestone, Sen_HK
dc.date.accessioned2010-09-06T08:18:18Z-
dc.date.available2010-09-06T08:18:18Z-
dc.date.issued2006en_HK
dc.identifier.citationBrain, 2006, v. 129 n. 11, p. 3042-3050en_HK
dc.identifier.issn0006-8950en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81482-
dc.description.abstractAlzheimer's disease is a common and devastating disease for which there is no readily available biomarker to aid diagnosis or to monitor disease progression. Biomarkers have been sought in CSF but no previous study has used two-dimensional gel electrophoresis coupled with mass spectrometry to seek biomarkers in peripheral tissue. We performed a case-control study of plasma using this proteomics approach to identify proteins that differ in the disease state relative to aged controls. For discovery-phase proteomics analysis, 50 people with Alzheimer's dementia were recruited through secondary services and 50 normal elderly controls through primary care. For validation purposes a total of 511 subjects with Alzheimer's disease and other neurodegenerative diseases and normal elderly controls were examined. Image analysis of the protein distribution of the gels alone identifies disease cases with 56% sensitivity and 80% specificity. Mass spectrometric analysis of the changes observed in two-dimensional electrophoresis identified a number of proteins previously implicated in the disease pathology, including complement factor H (CFH) precursor and α-2-macroglobulin (α- 2M). Using semi-quantitative immunoblotting, the elevation of CFH and α- 2M was shown to be specific for Alzheimer's disease and to correlate with disease severity although alternative assays would be necessary to improve sensitivity and specificity. These findings suggest that blood may be a rich source for biomarkers of Alzheimer's disease and that CFH, together with other proteins such as α- 2M may be a specific markers of this illness. © 2006 The Author(s).en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://brain.oxfordjournals.org/en_HK
dc.relation.ispartofBrainen_HK
dc.rightsBrain. Copyright © Oxford University Press.en_HK
dc.subjectAlzheimer's diseaseen_HK
dc.subjectBiomarkersen_HK
dc.subjectPlasmaen_HK
dc.subjectProteomicsen_HK
dc.subjectTwo-dimensional gel electrophoresis (2-DGE)en_HK
dc.titleProteome-based plasma biomarkers for Alzheimer's diseaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8950&volume=129&spage=3042&epage=3050&date=2006&atitle=Proteome-based+plasma+biomarkers+for+Alzheimer’s+diseaseen_HK
dc.identifier.emailSham, P: pcsham@hku.hken_HK
dc.identifier.authoritySham, P=rp00459en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/brain/awl279en_HK
dc.identifier.pmid17071923-
dc.identifier.scopuseid_2-s2.0-33750596715en_HK
dc.identifier.hkuros133771en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33750596715&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume129en_HK
dc.identifier.issue11en_HK
dc.identifier.spage3042en_HK
dc.identifier.epage3050en_HK
dc.identifier.isiWOS:000241783800024-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHye, A=6603434972en_HK
dc.identifier.scopusauthoridLynham, S=24724971300en_HK
dc.identifier.scopusauthoridThambisetty, M=6507073742en_HK
dc.identifier.scopusauthoridCausevic, M=6603628763en_HK
dc.identifier.scopusauthoridCampbell, J=7404875490en_HK
dc.identifier.scopusauthoridByers, HL=7006460214en_HK
dc.identifier.scopusauthoridHooper, C=13604722800en_HK
dc.identifier.scopusauthoridRijsdijk, F=6701830835en_HK
dc.identifier.scopusauthoridTabrizi, SJ=7006745684en_HK
dc.identifier.scopusauthoridBanner, S=7003546557en_HK
dc.identifier.scopusauthoridShaw, CE=35370282000en_HK
dc.identifier.scopusauthoridFoy, C=7004396232en_HK
dc.identifier.scopusauthoridPoppe, M=15052270500en_HK
dc.identifier.scopusauthoridArcher, N=7006158241en_HK
dc.identifier.scopusauthoridHamilton, G=7201369107en_HK
dc.identifier.scopusauthoridPowell, J=7403541196en_HK
dc.identifier.scopusauthoridBrown, RG=7406363771en_HK
dc.identifier.scopusauthoridSham, P=34573429300en_HK
dc.identifier.scopusauthoridWard, M=7403169022en_HK
dc.identifier.scopusauthoridLovestone, S=7005575001en_HK

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