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Article: Transfection of rat brain cells by electroporation

TitleTransfection of rat brain cells by electroporation
Authors
Keywordsβ-galactosidase
Culture
Electroporation
Neuron
Transfection
Issue Date1997
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jneumeth
Citation
Journal Of Neuroscience Methods, 1997, v. 75 n. 1, p. 29-32 How to Cite?
AbstractGenetic intervention is a powerful means for answering basic biological questions about molecular events taking place in brain cells underlying complex behaviors. Traditional transfection method hits proved difficult to apply to neuronal cells. We therefore described an alternative procedure for introducing DNA into cultured rat brain cells by electroporation. The various parameters involving the voltage, capacitance and electroporation buffer are investigated. We found that this transfection procedure is simple, reproducible and applicable to rat brain cells in vitro.
Persistent Identifierhttp://hdl.handle.net/10722/81371
ISSN
2015 Impact Factor: 2.053
2015 SCImago Journal Rankings: 1.217
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Hen_HK
dc.contributor.authorChan, STHen_HK
dc.contributor.authorTang, Fen_HK
dc.date.accessioned2010-09-06T08:16:52Z-
dc.date.available2010-09-06T08:16:52Z-
dc.date.issued1997en_HK
dc.identifier.citationJournal Of Neuroscience Methods, 1997, v. 75 n. 1, p. 29-32en_HK
dc.identifier.issn0165-0270en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81371-
dc.description.abstractGenetic intervention is a powerful means for answering basic biological questions about molecular events taking place in brain cells underlying complex behaviors. Traditional transfection method hits proved difficult to apply to neuronal cells. We therefore described an alternative procedure for introducing DNA into cultured rat brain cells by electroporation. The various parameters involving the voltage, capacitance and electroporation buffer are investigated. We found that this transfection procedure is simple, reproducible and applicable to rat brain cells in vitro.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jneumethen_HK
dc.relation.ispartofJournal of Neuroscience Methodsen_HK
dc.rightsJournal of Neuroscience methods. Copyright © Elsevier BV.en_HK
dc.subjectβ-galactosidaseen_HK
dc.subjectCultureen_HK
dc.subjectElectroporationen_HK
dc.subjectNeuronen_HK
dc.subjectTransfectionen_HK
dc.titleTransfection of rat brain cells by electroporationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0165-0270&volume=75&spage=29&epage=32&date=1997&atitle=Transfection+of+rat+brain+cells+by+electroporationen_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0165-0270(97)02259-0en_HK
dc.identifier.pmid9262140-
dc.identifier.scopuseid_2-s2.0-0030788328en_HK
dc.identifier.hkuros26102en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030788328&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume75en_HK
dc.identifier.issue1en_HK
dc.identifier.spage29en_HK
dc.identifier.epage32en_HK
dc.identifier.isiWOS:A1997XV09000005-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, H=24921636000en_HK
dc.identifier.scopusauthoridChan, STH=24368283200en_HK
dc.identifier.scopusauthoridTang, F=7201979770en_HK

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