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Article: Nestin-containing cells express glial fibrillary acidic protein in the proliferative regions of central nervous system of postnatal developing and adult mice

TitleNestin-containing cells express glial fibrillary acidic protein in the proliferative regions of central nervous system of postnatal developing and adult mice
Authors
KeywordsAstroglia
Central nervous system
Glial fibrillary acid protein
Intermediate filament
Nestin
Neural precursor
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/inca/publications/store/5/0/6/0/5/1/
Citation
Developmental Brain Research, 2002, v. 139 n. 1, p. 9-17 How to Cite?
AbstractWe are interested in the expression patterns of nestin, an embryonic intermediate filament that represent a neural precursor marker, in the mammalian central nervous system. With an immunohistochemical approach, distribution of nestin-containing cells and their colocalization with glial fibrillary acidic protein (GFAP) or neuronal nuclear specific protein (NeuN) were studied in adult and postnatal days 2-30 (P2-30) mice. Nestin-immunoreactivity was predominately distributed in certain proliferative regions, such as cerebral cortex, hippocampus, hypothalamus, subfornical organ, cerebellar cortex, area postrema, midline raphe glial structures, as well as ependymal and subependymal zones of the brain and spinal cord. The majority of nestin-immunoreactive cells, characterized by astroglial profiles of multiple and radial processes, showed a partial overlapping distribution with that of GFAP-immunoreactive astroglial cells. Double immunofluorescence confirmed that about 77% of these nestin-immunoreactive cells exhibited GFAP-immunoreactivity, indicating that a large percentage of nestin-expressing cells may have committed to astroglial cells. In developing mice, down-regulation of nestin expression was observed between P7 and P14. Although co-expression of nestin and NeuN occurred in cortical neurons of P2-7 mice, nestin-containing cells showing NeuN-immunoreactivity disappeared in CNS in older animals. Our results reveal the distribution pattern of nestin-containing neural precursors in the postnatal CNS and provide evidence on their differentiation fate to neurons and astrocytes, suggesting that nestin-containing glial cells may play an important role in remodeling and repairing in the postnatal and adult central nervous system. © Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/81353
ISSN
2007 Impact Factor: 1.783
2008 SCImago Journal Rankings: 1.249
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWei, LCen_HK
dc.contributor.authorShi, Men_HK
dc.contributor.authorChen, LWen_HK
dc.contributor.authorCao, Ren_HK
dc.contributor.authorZhang, Pen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2010-09-06T08:16:40Z-
dc.date.available2010-09-06T08:16:40Z-
dc.date.issued2002en_HK
dc.identifier.citationDevelopmental Brain Research, 2002, v. 139 n. 1, p. 9-17en_HK
dc.identifier.issn0165-3806en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81353-
dc.description.abstractWe are interested in the expression patterns of nestin, an embryonic intermediate filament that represent a neural precursor marker, in the mammalian central nervous system. With an immunohistochemical approach, distribution of nestin-containing cells and their colocalization with glial fibrillary acidic protein (GFAP) or neuronal nuclear specific protein (NeuN) were studied in adult and postnatal days 2-30 (P2-30) mice. Nestin-immunoreactivity was predominately distributed in certain proliferative regions, such as cerebral cortex, hippocampus, hypothalamus, subfornical organ, cerebellar cortex, area postrema, midline raphe glial structures, as well as ependymal and subependymal zones of the brain and spinal cord. The majority of nestin-immunoreactive cells, characterized by astroglial profiles of multiple and radial processes, showed a partial overlapping distribution with that of GFAP-immunoreactive astroglial cells. Double immunofluorescence confirmed that about 77% of these nestin-immunoreactive cells exhibited GFAP-immunoreactivity, indicating that a large percentage of nestin-expressing cells may have committed to astroglial cells. In developing mice, down-regulation of nestin expression was observed between P7 and P14. Although co-expression of nestin and NeuN occurred in cortical neurons of P2-7 mice, nestin-containing cells showing NeuN-immunoreactivity disappeared in CNS in older animals. Our results reveal the distribution pattern of nestin-containing neural precursors in the postnatal CNS and provide evidence on their differentiation fate to neurons and astrocytes, suggesting that nestin-containing glial cells may play an important role in remodeling and repairing in the postnatal and adult central nervous system. © Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/inca/publications/store/5/0/6/0/5/1/en_HK
dc.relation.ispartofDevelopmental Brain Researchen_HK
dc.rightsDevelopmental Brain Research . Copyright © Elsevier BV.en_HK
dc.subjectAstrogliaen_HK
dc.subjectCentral nervous systemen_HK
dc.subjectGlial fibrillary acid proteinen_HK
dc.subjectIntermediate filamenten_HK
dc.subjectNestinen_HK
dc.subjectNeural precursoren_HK
dc.titleNestin-containing cells express glial fibrillary acidic protein in the proliferative regions of central nervous system of postnatal developing and adult miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0165-3806&volume=139&spage=9&epage=17&date=2002&atitle=Nestin-containing+cells+express+glial+fibrillary+acidic+protein+in+the+proliferative+regions+of+central+nervous+system+of+postnatal+developing+and+adult+mice.++en_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0165-3806(02)00509-6en_HK
dc.identifier.pmid12414089-
dc.identifier.scopuseid_2-s2.0-0037111421en_HK
dc.identifier.hkuros81319en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037111421&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume139en_HK
dc.identifier.issue1en_HK
dc.identifier.spage9en_HK
dc.identifier.epage17en_HK
dc.identifier.isiWOS:000179420300002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWei, LC=7402951429en_HK
dc.identifier.scopusauthoridShi, M=36115245300en_HK
dc.identifier.scopusauthoridChen, LW=7409444941en_HK
dc.identifier.scopusauthoridCao, R=34567948400en_HK
dc.identifier.scopusauthoridZhang, P=9245727000en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK

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