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Article: Significant up-regulation of nestin protein in the neostriatum of MPTP-treated mice: Are the striatal astrocytes regionally activated after systemic MPTP administration?

TitleSignificant up-regulation of nestin protein in the neostriatum of MPTP-treated mice: Are the striatal astrocytes regionally activated after systemic MPTP administration?
Authors
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2002, v. 925 n. 1, p. 9-17 How to Cite?
AbstractWe are interested in the possible role of central glial cells in pathogenesis of Parkinson's disease of mammals. Parkinsonism model was induced by systemic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration, and the reactive glial cells were examined by immunocytochemical visualization of nestin protein in the brains and spinal cords of C57 mice. Abundant nestin-like immunoreactivity was predominately found in the caudate putamen of MPTP-treated mice and about 481-fold of nestin-like immunoreactive cells increased compared with that of control animals, indicating that significant up-regulation of nestin protein occurred in these regions. Majority of nestin-like immunoreactive cells characterized with astrocytic profiles of multiple, radical and hypotrophic processes, and showed a distribution and dynamic patterns similar to that of glial fibrillary acid protein (GFAP)-immunoreactive cells in the caudate putamen. Double immunofluorescence confirmed that 100% of nestin-like immunoreactive cells exhibited GFAP-immunoreactivity while nestin/GFAP double-labeled cells constituted about 84% of total GFAP-immunoreactive cells in the caudate putamen, indicating these nestin-like immunoreactive cells belong to a reactive population of the astrocytes. On the other hand, no obvious changes of nestin- or GFAP-like immunoreactivities were detected in the globus pallidus, the substantia nigra and the ventral tegmental area after MPTP-treatment. The results have provided morphological evidence for the regional activation of astrocytic glial cells following systemic MPTP administration, suggesting that a large population of reactive striatal astrocytes might play an important role in initial pathogenesis or acute stage of Parkinson's disease in mammals. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/81332
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, LWen_HK
dc.contributor.authorWei, LCen_HK
dc.contributor.authorQiu, Yen_HK
dc.contributor.authorLiu, HLen_HK
dc.contributor.authorRao, ZRen_HK
dc.contributor.authorJu, Gen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2010-09-06T08:16:27Z-
dc.date.available2010-09-06T08:16:27Z-
dc.date.issued2002en_HK
dc.identifier.citationBrain Research, 2002, v. 925 n. 1, p. 9-17en_HK
dc.identifier.issn0006-8993en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81332-
dc.description.abstractWe are interested in the possible role of central glial cells in pathogenesis of Parkinson's disease of mammals. Parkinsonism model was induced by systemic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration, and the reactive glial cells were examined by immunocytochemical visualization of nestin protein in the brains and spinal cords of C57 mice. Abundant nestin-like immunoreactivity was predominately found in the caudate putamen of MPTP-treated mice and about 481-fold of nestin-like immunoreactive cells increased compared with that of control animals, indicating that significant up-regulation of nestin protein occurred in these regions. Majority of nestin-like immunoreactive cells characterized with astrocytic profiles of multiple, radical and hypotrophic processes, and showed a distribution and dynamic patterns similar to that of glial fibrillary acid protein (GFAP)-immunoreactive cells in the caudate putamen. Double immunofluorescence confirmed that 100% of nestin-like immunoreactive cells exhibited GFAP-immunoreactivity while nestin/GFAP double-labeled cells constituted about 84% of total GFAP-immunoreactive cells in the caudate putamen, indicating these nestin-like immunoreactive cells belong to a reactive population of the astrocytes. On the other hand, no obvious changes of nestin- or GFAP-like immunoreactivities were detected in the globus pallidus, the substantia nigra and the ventral tegmental area after MPTP-treatment. The results have provided morphological evidence for the regional activation of astrocytic glial cells following systemic MPTP administration, suggesting that a large population of reactive striatal astrocytes might play an important role in initial pathogenesis or acute stage of Parkinson's disease in mammals. © 2002 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_HK
dc.relation.ispartofBrain Researchen_HK
dc.rightsBrain Research. Copyright © Elsevier BV.en_HK
dc.subject.mesh1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - pharmacologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAstrocytes - chemistry - metabolismen_HK
dc.subject.meshDopamine Agents - pharmacologyen_HK
dc.subject.meshGlial Fibrillary Acidic Protein - analysisen_HK
dc.subject.meshIntermediate Filament Proteins - analysis - metabolismen_HK
dc.subject.meshMPTP Poisoning - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshNeostriatum - cytology - metabolismen_HK
dc.subject.meshNerve Tissue Proteinsen_HK
dc.subject.meshSpinal Cord - cytology - metabolismen_HK
dc.titleSignificant up-regulation of nestin protein in the neostriatum of MPTP-treated mice: Are the striatal astrocytes regionally activated after systemic MPTP administration?en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=925&spage=9&epage=17&date=2002&atitle=Significant+up-regulation+of+nestin+protein+in+the+neostriatum+of+MPTP-treated+mice:+Are+the+striatal+astrocytes+regionally+activated+after+systemic+MPTP+administration?en_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-8993(01)03253-Xen_HK
dc.identifier.pmid11755896-
dc.identifier.scopuseid_2-s2.0-0037126959en_HK
dc.identifier.hkuros72004en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037126959&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume925en_HK
dc.identifier.issue1en_HK
dc.identifier.spage9en_HK
dc.identifier.epage17en_HK
dc.identifier.isiWOS:000173275100002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, LW=7409444941en_HK
dc.identifier.scopusauthoridWei, LC=7402951429en_HK
dc.identifier.scopusauthoridQiu, Y=7403279738en_HK
dc.identifier.scopusauthoridLiu, HL=36066506000en_HK
dc.identifier.scopusauthoridRao, ZR=7102549067en_HK
dc.identifier.scopusauthoridJu, G=26642903700en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK

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