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Article: Interstitial adenosine concentration in rat red or white skeletal muscle during systemic hypoxia or contractions

TitleInterstitial adenosine concentration in rat red or white skeletal muscle during systemic hypoxia or contractions
Authors
Issue Date2001
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EPH
Citation
Experimental Physiology, 2001, v. 86 n. 5, p. 593-598 How to Cite?
AbstractInterstitial adenosine concentrations in red soleus (SL) or white extensor digitorum longus (EDL) muscles of anaesthetised rats were determined using microdialysis and HPLC. Systemic hypoxia was induced by ventilating the animals with 10% oxygen in nitrogen for 15 min: arterial PO2 decreased from 111.8 ± 10.9 to 42.2 ± 4.3 mmHg (n = 4; P < 0.01) and mean systemic arterial blood pressure from 97.6 ± 4.9 to 59.0 ± 3.6 mmHg (n = 22; P < 0.001). The interstitial adenosine concentration was not significantly changed from its control values of 294 ± 44 nM (n = 20) in EDL and 302 ± 36 nM (n = 20) in SL during hypoxia or the recovery period. The interstitial lactate concentration did not change in the early part of the hypoxia but increased from 1.0 ± 0.2 to 1.4 ± 0.3 mM (n = 6; P < 0.05) in SL and from 2.0 ± 0.4 to 2.4 ± 0.4 mM (n = 6; P < 0.05) in EDL during the later part of the hypoxia, and remained elevated in the recovery period. Muscle contractions (2 Hz for 15 min) produced a transient increase in the interstitial adenosine concentration of SL from 150 ± 35 to 244 ± 75 nM (n = 10; P < 0.05) during the first 5 min of stimulation. In EDL the interstitial adenosine concentration increased from 145 ± 50 to 435 ± 144 nM (n = 10; P < 0.05) in the later part of the contraction and remained elevated in the early part of the recovery period. These data suggest that: (i) in systemic hypoxia adenosine does not appear in the interstitial space, which rules out its release from skeletal muscle, although it may be formed by the vascular tissues in this condition; (ii) adenosine is formed in the interstitial space of skeletal muscle during muscle contractions; (iii) there is slow clearance of adenosine from the interstitial space of white muscle, perhaps due to the low vascularity of the tissue.
Persistent Identifierhttp://hdl.handle.net/10722/81325
ISSN
2015 Impact Factor: 2.818
2015 SCImago Journal Rankings: 1.390
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, SMen_HK
dc.contributor.authorMo, FMen_HK
dc.contributor.authorBallard, HJen_HK
dc.date.accessioned2010-09-06T08:16:22Z-
dc.date.available2010-09-06T08:16:22Z-
dc.date.issued2001en_HK
dc.identifier.citationExperimental Physiology, 2001, v. 86 n. 5, p. 593-598en_HK
dc.identifier.issn0958-0670en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81325-
dc.description.abstractInterstitial adenosine concentrations in red soleus (SL) or white extensor digitorum longus (EDL) muscles of anaesthetised rats were determined using microdialysis and HPLC. Systemic hypoxia was induced by ventilating the animals with 10% oxygen in nitrogen for 15 min: arterial PO2 decreased from 111.8 ± 10.9 to 42.2 ± 4.3 mmHg (n = 4; P < 0.01) and mean systemic arterial blood pressure from 97.6 ± 4.9 to 59.0 ± 3.6 mmHg (n = 22; P < 0.001). The interstitial adenosine concentration was not significantly changed from its control values of 294 ± 44 nM (n = 20) in EDL and 302 ± 36 nM (n = 20) in SL during hypoxia or the recovery period. The interstitial lactate concentration did not change in the early part of the hypoxia but increased from 1.0 ± 0.2 to 1.4 ± 0.3 mM (n = 6; P < 0.05) in SL and from 2.0 ± 0.4 to 2.4 ± 0.4 mM (n = 6; P < 0.05) in EDL during the later part of the hypoxia, and remained elevated in the recovery period. Muscle contractions (2 Hz for 15 min) produced a transient increase in the interstitial adenosine concentration of SL from 150 ± 35 to 244 ± 75 nM (n = 10; P < 0.05) during the first 5 min of stimulation. In EDL the interstitial adenosine concentration increased from 145 ± 50 to 435 ± 144 nM (n = 10; P < 0.05) in the later part of the contraction and remained elevated in the early part of the recovery period. These data suggest that: (i) in systemic hypoxia adenosine does not appear in the interstitial space, which rules out its release from skeletal muscle, although it may be formed by the vascular tissues in this condition; (ii) adenosine is formed in the interstitial space of skeletal muscle during muscle contractions; (iii) there is slow clearance of adenosine from the interstitial space of white muscle, perhaps due to the low vascularity of the tissue.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EPHen_HK
dc.relation.ispartofExperimental Physiologyen_HK
dc.rightsExperimental Physiology. Copyright © Blackwell Publishing Ltd.en_HK
dc.titleInterstitial adenosine concentration in rat red or white skeletal muscle during systemic hypoxia or contractionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0958-0670&volume=86&spage=593&epage=598&date=2001&atitle=Interstitial+adenosine+concentration+in+rat+red+or+white+skeletal+muscle+during+systemic+hypoxia+or+contractionsen_HK
dc.identifier.emailBallard, HJ: ballard@hkucc.hku.hken_HK
dc.identifier.authorityBallard, HJ=rp00367en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1113/eph8602226en_HK
dc.identifier.pmid11571486-
dc.identifier.scopuseid_2-s2.0-0034788028en_HK
dc.identifier.hkuros72900en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034788028&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume86en_HK
dc.identifier.issue5en_HK
dc.identifier.spage593en_HK
dc.identifier.epage598en_HK
dc.identifier.isiWOS:000171414600008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLo, SM=7401542420en_HK
dc.identifier.scopusauthoridMo, FM=7005059536en_HK
dc.identifier.scopusauthoridBallard, HJ=7005286310en_HK

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