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Article: 5-HT excites globus pallidus neurons by multiple receptor mechanisms

Title5-HT excites globus pallidus neurons by multiple receptor mechanisms
Authors
Keywords5-HT
5-HT receptors
globus pallidus
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 2008, v. 151 n. 2, p. 439-451 How to Cite?
AbstractAnatomical and neurochemical studies indicated that the globus pallidus receives serotonergic innervation from raphe nuclei but the membrane effects of 5-HT on globus pallidus neurons are not entirely clear. We address this question by applying whole-cell patch-clamp recordings on globus pallidus neurons in immature rat brain slices. Under current-clamp recording, 5-HT depolarized globus pallidus neurons and increased their firing rate, an action blocked by both 5-HT4 and 5-HT7 receptor antagonists and attributable to an increase in cation conductance(s). Further experiments indicated that 5-HT enhanced the hyperpolarization-activated inward conductance which is blocked by 5-HT7 receptor antagonist. To determine if 5-HT exerts any presynaptic effects on GABAergic and glutamatergic inputs, the actions of 5-HT on synaptic currents were studied. At 10 μM, 5-HT increased the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) but had no effect on both the frequency and amplitude of miniature inhibitory postsynaptic currents (mIPSCs). However, 5-HT at a higher concentration (50 μM) decreased the frequency but not the amplitude of the mIPSCs, indicating an inhibition of GABA release from the presynaptic terminals. This effect was sensitive to 5-HT1B receptor antagonist. In addition to the presynaptic effects on GABAergic neurotransmission, 5-HT at 50 μM had no consistent effects on glutamatergic neurotransmission, significantly increased the frequency of miniature excitatory postsynaptic currents (mEPSCs) in 4 of 11 neurons and decreased the frequency of mEPSCs in 3 of 11 neurons. In conclusion, we found that 5-HT could modulate the excitability of globus pallidus neurons by both pre- and post-synaptic mechanisms. In view of the extensive innervation by globus pallidus neurons on other basal ganglia nuclei, this action of 5-HT originated from the raphe may have a profound effect on the operation of the entire basal ganglia network. © 2008 IBRO.
Persistent Identifierhttp://hdl.handle.net/10722/81315
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Len_HK
dc.contributor.authorYung, KKLen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorYung, WHen_HK
dc.date.accessioned2010-09-06T08:16:16Z-
dc.date.available2010-09-06T08:16:16Z-
dc.date.issued2008en_HK
dc.identifier.citationNeuroscience, 2008, v. 151 n. 2, p. 439-451en_HK
dc.identifier.issn0306-4522en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81315-
dc.description.abstractAnatomical and neurochemical studies indicated that the globus pallidus receives serotonergic innervation from raphe nuclei but the membrane effects of 5-HT on globus pallidus neurons are not entirely clear. We address this question by applying whole-cell patch-clamp recordings on globus pallidus neurons in immature rat brain slices. Under current-clamp recording, 5-HT depolarized globus pallidus neurons and increased their firing rate, an action blocked by both 5-HT4 and 5-HT7 receptor antagonists and attributable to an increase in cation conductance(s). Further experiments indicated that 5-HT enhanced the hyperpolarization-activated inward conductance which is blocked by 5-HT7 receptor antagonist. To determine if 5-HT exerts any presynaptic effects on GABAergic and glutamatergic inputs, the actions of 5-HT on synaptic currents were studied. At 10 μM, 5-HT increased the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) but had no effect on both the frequency and amplitude of miniature inhibitory postsynaptic currents (mIPSCs). However, 5-HT at a higher concentration (50 μM) decreased the frequency but not the amplitude of the mIPSCs, indicating an inhibition of GABA release from the presynaptic terminals. This effect was sensitive to 5-HT1B receptor antagonist. In addition to the presynaptic effects on GABAergic neurotransmission, 5-HT at 50 μM had no consistent effects on glutamatergic neurotransmission, significantly increased the frequency of miniature excitatory postsynaptic currents (mEPSCs) in 4 of 11 neurons and decreased the frequency of mEPSCs in 3 of 11 neurons. In conclusion, we found that 5-HT could modulate the excitability of globus pallidus neurons by both pre- and post-synaptic mechanisms. In view of the extensive innervation by globus pallidus neurons on other basal ganglia nuclei, this action of 5-HT originated from the raphe may have a profound effect on the operation of the entire basal ganglia network. © 2008 IBRO.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscienceen_HK
dc.relation.ispartofNeuroscienceen_HK
dc.rightsNeuroscience. Copyright © Elsevier BV.en_HK
dc.subject5-HTen_HK
dc.subject5-HT receptorsen_HK
dc.subjectglobus pallidusen_HK
dc.title5-HT excites globus pallidus neurons by multiple receptor mechanismsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0306-4522&volume=151&spage=439&epage=451&date=2008&atitle=5-HT+excites+globus+pallidus+neurons+by+multiple+receptor+mechanisms.++en_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.neuroscience.2007.11.003en_HK
dc.identifier.pmid18082329-
dc.identifier.scopuseid_2-s2.0-37849014109en_HK
dc.identifier.hkuros149583en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-37849014109&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume151en_HK
dc.identifier.issue2en_HK
dc.identifier.spage439en_HK
dc.identifier.epage451en_HK
dc.identifier.isiWOS:000252644600014-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, L=25652992200en_HK
dc.identifier.scopusauthoridYung, KKL=13605496000en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridYung, WH=7103137893en_HK

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