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Article: Quantitative study of the coexpression of Fos and N-methyl-D aspartate (NMDA) receptor subunits in otolith-related vestibular nuclear neurons of rats

TitleQuantitative study of the coexpression of Fos and N-methyl-D aspartate (NMDA) receptor subunits in otolith-related vestibular nuclear neurons of rats
Authors
KeywordsC-fos
Immunohistochemistry
NMDA receptor
Off-vertical axis rotation
Utricle
Vestibular nuclei
Issue Date2003
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal Of Comparative Neurology, 2003, v. 460 n. 2, p. 292-301 How to Cite?
AbstractThe expression of NMDA receptor subunits (NR1 and NR2A/B) was demonstrated immunocytochemically in otolith-related neurons within the vestibular nuclear complex and its subnuclei of conscious Sprague-Dawley adult rats. All experimental animals were subjected to constant velocity off-vertical axis rotation (OVAR). The rotating gravity vector during OVAR sequentially activates hair cells on all sectors of the utricular maculae; neurons so activated within the vestibular nuclei were denoted by the expression of Fos protein. Control animals, i.e., labyrinthectomized rats subjected to OVAR and normal rats that remained stationary, showed only a few sporadically scattered labeled neurons. In the brainstem of normal rats subjected to OVAR, a high density of Fos-immunoreactive (Fos-ir) neurons was found in the vestibular nuclear complex (namely, spinal vestibular nucleus, SpVe; medial vestibular nucleus, Mve; superior vestibular nucleus, SuVe) and subnuclei (namely, group x and group y), whereas a lower density was found in the lateral vestibular nucleus (LVe). A double-immunofluoreseence study indicated that both NR1 and NR2A/B subunits were highly expressed in Fos-ir neurons within the vestibular nuclei. Fos/NR1 or Fos/NR2A/B double-labeled neurons constitute over three-quarters of the total number of Fos-ir neurons in SpVe, MVe, LVe, SuVe, and groups x and y. Our findings suggest that NMDA-type ionotropic glutamate receptors play a key role in the OVAR-induced neuronal activation of the vestibular nuclei, thus providing a morphological basis for further study of glutamatergic central otolith neurons and their involvement in sensorimotor regulation and autonomic functions of rats. © 2003 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/81297
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, LWen_HK
dc.contributor.authorLai, CHen_HK
dc.contributor.authorLaw, HYen_HK
dc.contributor.authorYung, KKLen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2010-09-06T08:16:04Z-
dc.date.available2010-09-06T08:16:04Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Comparative Neurology, 2003, v. 460 n. 2, p. 292-301en_HK
dc.identifier.issn0021-9967en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81297-
dc.description.abstractThe expression of NMDA receptor subunits (NR1 and NR2A/B) was demonstrated immunocytochemically in otolith-related neurons within the vestibular nuclear complex and its subnuclei of conscious Sprague-Dawley adult rats. All experimental animals were subjected to constant velocity off-vertical axis rotation (OVAR). The rotating gravity vector during OVAR sequentially activates hair cells on all sectors of the utricular maculae; neurons so activated within the vestibular nuclei were denoted by the expression of Fos protein. Control animals, i.e., labyrinthectomized rats subjected to OVAR and normal rats that remained stationary, showed only a few sporadically scattered labeled neurons. In the brainstem of normal rats subjected to OVAR, a high density of Fos-immunoreactive (Fos-ir) neurons was found in the vestibular nuclear complex (namely, spinal vestibular nucleus, SpVe; medial vestibular nucleus, Mve; superior vestibular nucleus, SuVe) and subnuclei (namely, group x and group y), whereas a lower density was found in the lateral vestibular nucleus (LVe). A double-immunofluoreseence study indicated that both NR1 and NR2A/B subunits were highly expressed in Fos-ir neurons within the vestibular nuclei. Fos/NR1 or Fos/NR2A/B double-labeled neurons constitute over three-quarters of the total number of Fos-ir neurons in SpVe, MVe, LVe, SuVe, and groups x and y. Our findings suggest that NMDA-type ionotropic glutamate receptors play a key role in the OVAR-induced neuronal activation of the vestibular nuclei, thus providing a morphological basis for further study of glutamatergic central otolith neurons and their involvement in sensorimotor regulation and autonomic functions of rats. © 2003 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_HK
dc.relation.ispartofJournal of Comparative Neurologyen_HK
dc.subjectC-fosen_HK
dc.subjectImmunohistochemistryen_HK
dc.subjectNMDA receptoren_HK
dc.subjectOff-vertical axis rotationen_HK
dc.subjectUtricleen_HK
dc.subjectVestibular nucleien_HK
dc.titleQuantitative study of the coexpression of Fos and N-methyl-D aspartate (NMDA) receptor subunits in otolith-related vestibular nuclear neurons of ratsen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, CH: chlaib@hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityLai, CH=rp00396en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cne.10657en_HK
dc.identifier.pmid12687692en_HK
dc.identifier.scopuseid_2-s2.0-0037467553en_HK
dc.identifier.hkuros81357en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037467553&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume460en_HK
dc.identifier.issue2en_HK
dc.identifier.spage292en_HK
dc.identifier.epage301en_HK
dc.identifier.isiWOS:000182426800013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, LW=7409444941en_HK
dc.identifier.scopusauthoridLai, CH=7403086597en_HK
dc.identifier.scopusauthoridLaw, HY=7101939405en_HK
dc.identifier.scopusauthoridYung, KKL=13605496000en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK

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