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Article: Co-localization of NMDA receptors and AMPA receptors in neurons of the vestibular nuclei of rats

TitleCo-localization of NMDA receptors and AMPA receptors in neurons of the vestibular nuclei of rats
Authors
KeywordsAMPA receptor
Co-localization
Immunocytochemistry
NMDA receptor
Vestibular nucleus
Issue Date2000
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2000, v. 884 n. 1-2, p. 87-97 How to Cite?
AbstractWe are interested in studying the co-localization of NMDA glutamate receptor subunits (NR1, NR2A/B) and AMPA glutamate receptor subunits (GluR1, GluR2, GluR2/3 and GluR4) in individual neurons of the rat vestibular nuclei. Immunoreactivity for NR1, NR2A/B, GluR1, GluR2, GluR2/3 and GluR4 was found in the somata and dendrites of neurons in the four major subdivisions (superior, medial, lateral, and spinal vestibular nuclei) and in two minor groups (groups x and y) of the vestibular nuclei. Double immunofluorescence showed that all the NR1-containing neurons exhibited NR2A/B immunoreactivity, indicating that native NMDA receptors are composed of NR1 and NR2A/B in a hetero-oligomeric configuration. Co-expression of NMDA receptor subunits and AMPA receptor subunits was demonstrated by double labeling of NR1/GluR1, NR1/GluR2/3, NR1/GluR4 and NR2A/B/GluR2 in individual vestibular nuclear neurons. All NR1-containing neurons expressed GluR2/3 immunoreactivity, and all NR2A/B-containing neurons expressed GluR2 immunoreactivity. However, only about 52% of NR1-immunoreactive neurons exhibited GluR1 immunoreactivity and 46% of NR1-containing neurons showed GluR4 immunoreactivity. The present data reveal that NMDA receptors are co-localized with variants of AMPA receptors in a large proportion of vestibular nuclear neurons. These results suggest that cross-modulation between NMDA receptors and AMPA receptors may occur in individual neurons of the vestibular nuclei during glutamate-mediated excitatory neurotransmission and may in turn contribute to synaptic plasticity within the vestibular nuclei. Copyright (C) 2000 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/81274
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, LWen_HK
dc.contributor.authorYung, KKLen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2010-09-06T08:15:48Z-
dc.date.available2010-09-06T08:15:48Z-
dc.date.issued2000en_HK
dc.identifier.citationBrain Research, 2000, v. 884 n. 1-2, p. 87-97en_HK
dc.identifier.issn0006-8993en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81274-
dc.description.abstractWe are interested in studying the co-localization of NMDA glutamate receptor subunits (NR1, NR2A/B) and AMPA glutamate receptor subunits (GluR1, GluR2, GluR2/3 and GluR4) in individual neurons of the rat vestibular nuclei. Immunoreactivity for NR1, NR2A/B, GluR1, GluR2, GluR2/3 and GluR4 was found in the somata and dendrites of neurons in the four major subdivisions (superior, medial, lateral, and spinal vestibular nuclei) and in two minor groups (groups x and y) of the vestibular nuclei. Double immunofluorescence showed that all the NR1-containing neurons exhibited NR2A/B immunoreactivity, indicating that native NMDA receptors are composed of NR1 and NR2A/B in a hetero-oligomeric configuration. Co-expression of NMDA receptor subunits and AMPA receptor subunits was demonstrated by double labeling of NR1/GluR1, NR1/GluR2/3, NR1/GluR4 and NR2A/B/GluR2 in individual vestibular nuclear neurons. All NR1-containing neurons expressed GluR2/3 immunoreactivity, and all NR2A/B-containing neurons expressed GluR2 immunoreactivity. However, only about 52% of NR1-immunoreactive neurons exhibited GluR1 immunoreactivity and 46% of NR1-containing neurons showed GluR4 immunoreactivity. The present data reveal that NMDA receptors are co-localized with variants of AMPA receptors in a large proportion of vestibular nuclear neurons. These results suggest that cross-modulation between NMDA receptors and AMPA receptors may occur in individual neurons of the vestibular nuclei during glutamate-mediated excitatory neurotransmission and may in turn contribute to synaptic plasticity within the vestibular nuclei. Copyright (C) 2000 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_HK
dc.relation.ispartofBrain Researchen_HK
dc.rightsBrain Research. Copyright © Elsevier BV.en_HK
dc.subjectAMPA receptoren_HK
dc.subjectCo-localizationen_HK
dc.subjectImmunocytochemistryen_HK
dc.subjectNMDA receptoren_HK
dc.subjectVestibular nucleusen_HK
dc.titleCo-localization of NMDA receptors and AMPA receptors in neurons of the vestibular nuclei of ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=884&spage=87&epage=97&date=2000&atitle=Co-localization+of+NMDA+receptors+and+AMPA+receptors+in+neurons+of+the+vestibular+nucleus+of+ratsen_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-8993(00)02913-9en_HK
dc.identifier.pmid11082490-
dc.identifier.scopuseid_2-s2.0-0034711560en_HK
dc.identifier.hkuros60096en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034711560&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume884en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage87en_HK
dc.identifier.epage97en_HK
dc.identifier.isiWOS:000165749200010-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, LW=7409444941en_HK
dc.identifier.scopusauthoridYung, KKL=13605496000en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK

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