File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: GABA-B receptor activation in the rat globus pallidus potently suppresses pentylenetetrazol-induced tonic seizures

TitleGABA-B receptor activation in the rat globus pallidus potently suppresses pentylenetetrazol-induced tonic seizures
Authors
KeywordsBaclofen
Epilepsy
Globus pallidus
Pentylenetetrazol
Tiagabine
Zolpidem
Issue Date2004
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1021-7770
Citation
Journal Of Biomedical Science, 2004, v. 11 n. 4, p. 457-464 How to Cite?
AbstractTo determine the involvement of the globus pallidus in mediating epilepsy, the effects of microinjection of a GABA uptake blocker (tiagabine), a benzodiazepine agonist (zolpidem) and a GABA-B receptor agonist (baclofen) on pentylenetetrazol (PTZ)-induced tonic seizure were examined in adult rats. Administration of PTZ induced tonic seizures in all control animals, accompanied with a 100% mortality rate. Pretreatment with bilateral intrapallidal microinjection of tiagabine (1 mM) suppressed the incidence of tonic seizures to 67.7% and reduced the mortality rate to 16.7%. Furthermore, the latency to tonic seizures was 1,275 ± 277 s, which was significantly longer than that of the corresponding control animals (319 ± 225 s). On the other hand, administration of zolpidem (1 mM) was without significant effects on the mortality rate, the incidence and latency of the tonic seizure. In sharp contrast, microinjection of baclofen (1mM) completely suppressed PTZ-induced tonic seizures and reduced the mortality rate to 0%. This effect was largely abolished by co-injection of the GABA-B receptor antagonist CGP55845. To elucidate the direct cellular action of baclofen, the excitability and membrane potential of globus pallidus neurons were studied by cell-attached and whole-cell patch-clamp recordings in the brain slice. Bath application of baclofen (50 μM) significantly reduced the firing of these neurons, and was often accompanied by a clear membrane hyperpolarization, in a CGP55845-sensitive manner. These data suggest that activation of GABA-B receptors in globus pallidus could significantly modulate PTZ-induced tonic seizures. Copyright © 2004 National Science Council, ROC and S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/81273
ISSN
2015 Impact Factor: 2.935
2015 SCImago Journal Rankings: 1.280
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Len_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorYung, WHen_HK
dc.date.accessioned2010-09-06T08:15:48Z-
dc.date.available2010-09-06T08:15:48Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Biomedical Science, 2004, v. 11 n. 4, p. 457-464en_HK
dc.identifier.issn1021-7770en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81273-
dc.description.abstractTo determine the involvement of the globus pallidus in mediating epilepsy, the effects of microinjection of a GABA uptake blocker (tiagabine), a benzodiazepine agonist (zolpidem) and a GABA-B receptor agonist (baclofen) on pentylenetetrazol (PTZ)-induced tonic seizure were examined in adult rats. Administration of PTZ induced tonic seizures in all control animals, accompanied with a 100% mortality rate. Pretreatment with bilateral intrapallidal microinjection of tiagabine (1 mM) suppressed the incidence of tonic seizures to 67.7% and reduced the mortality rate to 16.7%. Furthermore, the latency to tonic seizures was 1,275 ± 277 s, which was significantly longer than that of the corresponding control animals (319 ± 225 s). On the other hand, administration of zolpidem (1 mM) was without significant effects on the mortality rate, the incidence and latency of the tonic seizure. In sharp contrast, microinjection of baclofen (1mM) completely suppressed PTZ-induced tonic seizures and reduced the mortality rate to 0%. This effect was largely abolished by co-injection of the GABA-B receptor antagonist CGP55845. To elucidate the direct cellular action of baclofen, the excitability and membrane potential of globus pallidus neurons were studied by cell-attached and whole-cell patch-clamp recordings in the brain slice. Bath application of baclofen (50 μM) significantly reduced the firing of these neurons, and was often accompanied by a clear membrane hyperpolarization, in a CGP55845-sensitive manner. These data suggest that activation of GABA-B receptors in globus pallidus could significantly modulate PTZ-induced tonic seizures. Copyright © 2004 National Science Council, ROC and S. Karger AG, Basel.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1021-7770en_HK
dc.relation.ispartofJournal of Biomedical Scienceen_HK
dc.subjectBaclofenen_HK
dc.subjectEpilepsyen_HK
dc.subjectGlobus pallidusen_HK
dc.subjectPentylenetetrazolen_HK
dc.subjectTiagabineen_HK
dc.subjectZolpidemen_HK
dc.titleGABA-B receptor activation in the rat globus pallidus potently suppresses pentylenetetrazol-induced tonic seizuresen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1021-7770&volume=11&spage=457&epage=464&date=2004&atitle=GABA-B+receptor+activation+in+the+rat+globus+pallidus+potently+suppresses+pentylenetetrazol-induced+tonic+seizureen_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000077895en_HK
dc.identifier.pmid15153780-
dc.identifier.scopuseid_2-s2.0-2642517883en_HK
dc.identifier.hkuros106585en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2642517883&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue4en_HK
dc.identifier.spage457en_HK
dc.identifier.epage464en_HK
dc.identifier.isiWOS:000221584300004-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, L=15758988300en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridYung, WH=7103137893en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats