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Article: Evidence for the existence of a constitutive nitric oxide synthase in vascular smooth muscle

TitleEvidence for the existence of a constitutive nitric oxide synthase in vascular smooth muscle
Authors
KeywordsAorta
Caveolae
Cell membrane
Endothelium
Field stimulation
Nitric oxide synthase
Vascular smooth muscle
Issue Date2002
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEP
Citation
Clinical And Experimental Pharmacology And Physiology, 2002, v. 29 n. 8, p. 725-727 How to Cite?
Abstract1. We have identified a neuronal nitric oxide synthase (NOS)-like constitutive form of NOS in vascular smooth muscle (VSM) using a functional contractility approach as well as immunohistochemical methods. 2. NG-Nitro-L-arginine methyl ester, NG-monomethyl-L-arginine and NG-nitro-L-arginine (L-NOARG), the competitive inhibitors of NOS, inhibited Mg2+-induced relaxation of de-endothelialized rat aorta precontracted with phenylephrine (PE). This Mg2+ relaxation of VSM was not affected by inhibitors of inducible NOS. 3. Electrical field stimulation (EFS; 30-70 Hz) caused relaxation of rat aorta in the presence of tetrodotoxin (therefore not a neurogenic effect) and this EFS relaxation was effectively inhibited by L-NOARG, oxyhemoglobin and methylene blue. 4. Immunohistochemical studies of dog saphenous vein using antibodies raised against neuronal NOS indicated prominent staining along the plasmalemma in a punctate pattern similar to the distribution of antibodies against caveolin-1, a major constituent of the plasmalemmal caveolae. 5. We propose that a constitutive NOS of non-endothelial, non-neuronal origin is present in a special caveolae domain of VSM cell membranes and could be activated by an ionic mechanism yet to be characterized.
Persistent Identifierhttp://hdl.handle.net/10722/81267
ISSN
2012 Impact Factor: 2.16
2015 SCImago Journal Rankings: 0.944
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheah, LSen_HK
dc.contributor.authorGwee, MCEen_HK
dc.contributor.authorDas, Ren_HK
dc.contributor.authorBallard, Hen_HK
dc.contributor.authorYang, YFen_HK
dc.contributor.authorDaniel, EEen_HK
dc.contributor.authorKwan, CYen_HK
dc.date.accessioned2010-09-06T08:15:44Z-
dc.date.available2010-09-06T08:15:44Z-
dc.date.issued2002en_HK
dc.identifier.citationClinical And Experimental Pharmacology And Physiology, 2002, v. 29 n. 8, p. 725-727en_HK
dc.identifier.issn0305-1870en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81267-
dc.description.abstract1. We have identified a neuronal nitric oxide synthase (NOS)-like constitutive form of NOS in vascular smooth muscle (VSM) using a functional contractility approach as well as immunohistochemical methods. 2. NG-Nitro-L-arginine methyl ester, NG-monomethyl-L-arginine and NG-nitro-L-arginine (L-NOARG), the competitive inhibitors of NOS, inhibited Mg2+-induced relaxation of de-endothelialized rat aorta precontracted with phenylephrine (PE). This Mg2+ relaxation of VSM was not affected by inhibitors of inducible NOS. 3. Electrical field stimulation (EFS; 30-70 Hz) caused relaxation of rat aorta in the presence of tetrodotoxin (therefore not a neurogenic effect) and this EFS relaxation was effectively inhibited by L-NOARG, oxyhemoglobin and methylene blue. 4. Immunohistochemical studies of dog saphenous vein using antibodies raised against neuronal NOS indicated prominent staining along the plasmalemma in a punctate pattern similar to the distribution of antibodies against caveolin-1, a major constituent of the plasmalemmal caveolae. 5. We propose that a constitutive NOS of non-endothelial, non-neuronal origin is present in a special caveolae domain of VSM cell membranes and could be activated by an ionic mechanism yet to be characterized.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEPen_HK
dc.relation.ispartofClinical and Experimental Pharmacology and Physiologyen_HK
dc.subjectAortaen_HK
dc.subjectCaveolaeen_HK
dc.subjectCell membraneen_HK
dc.subjectEndotheliumen_HK
dc.subjectField stimulationen_HK
dc.subjectNitric oxide synthaseen_HK
dc.subjectVascular smooth muscleen_HK
dc.titleEvidence for the existence of a constitutive nitric oxide synthase in vascular smooth muscleen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0305-1870&volume=29&spage=725&epage=727&date=2002&atitle=Evidence+for+the+existence+of+a+constitutive+nitric+oxide+synthase+in+vascular+smooth+muscle.en_HK
dc.identifier.emailBallard, H: ballard@hkucc.hku.hken_HK
dc.identifier.authorityBallard, H=rp00367en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1440-1681.2002.03707.xen_HK
dc.identifier.pmid12100009-
dc.identifier.scopuseid_2-s2.0-0035988924en_HK
dc.identifier.hkuros113235en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035988924&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue8en_HK
dc.identifier.spage725en_HK
dc.identifier.epage727en_HK
dc.identifier.isiWOS:000176639600017-
dc.publisher.placeAustraliaen_HK
dc.identifier.scopusauthoridCheah, LS=7005063996en_HK
dc.identifier.scopusauthoridGwee, MCE=36971965200en_HK
dc.identifier.scopusauthoridDas, R=7202061890en_HK
dc.identifier.scopusauthoridBallard, H=7005286310en_HK
dc.identifier.scopusauthoridYang, YF=18937941900en_HK
dc.identifier.scopusauthoridDaniel, EE=35474017600en_HK
dc.identifier.scopusauthoridKwan, CY=7201421224en_HK

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