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Article: Nestin small interfering RNA (siRNA) reduces cell growth in cultured astrocytoma cells

TitleNestin small interfering RNA (siRNA) reduces cell growth in cultured astrocytoma cells
Authors
KeywordsAstrocytoma
Cell culture
Cell growth
Nestin
Small interfering RNA
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2008, v. 1196, p. 103-112 How to Cite?
AbstractNestin is an embryonic intermediate filament that transiently expressed in the neural stem/progenitor cells in the developing central nervous system (CNS). Growing evidence has shown that abundant expression of nestin also occurs in both pathological glial-derived tumor cells and reactive astrocytes in various CNS injuries, implying that nestin may play a crucial role in cell growth or proliferation of astrocyte-derived tumor cells. In the present study, we have investigated the possible role of nestin expression in cell growth or survival of CNS tumor cells by using novel small interfering RNA (siRNA) method in cell culture of rat astrocytoma C6 cell line. The nestin expression and cell growth of the cultured astrocytoma cells were examined after nestin siRNA duplex was delivered by cell transfection for 6 h and cell culture was maintained for 48 h. It revealed an effective suppression influence of nestin siRNA on cell growth of cultured astrocytoma cells in a dose-dependent manner. Quantitative data analysis showed that the doses of nestin siRNA at 30-120 nM significantly decreased both cell numbers and expression levels of nestin mRNA and protein. The nestin siRNA also suppressed expression of cellular glial fibrillary acid protein but showed no obvious influence on expression level of Ki-67 protein (a cell proliferation marker). This study has provided in vitro evidence that nestin siRNA can effectively block nestin expression and reduce cell growth of the cultured C6 astrocytoma cells, strongly suggesting that nestin siRNA-induced suppression of tumor cell growth may provide a potential novel clinical therapy against CNS astroglioma events. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/81242
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWei, LCen_HK
dc.contributor.authorShi, Men_HK
dc.contributor.authorCao, Ren_HK
dc.contributor.authorChen, LWen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2010-09-06T08:15:26Z-
dc.date.available2010-09-06T08:15:26Z-
dc.date.issued2008en_HK
dc.identifier.citationBrain Research, 2008, v. 1196, p. 103-112en_HK
dc.identifier.issn0006-8993en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81242-
dc.description.abstractNestin is an embryonic intermediate filament that transiently expressed in the neural stem/progenitor cells in the developing central nervous system (CNS). Growing evidence has shown that abundant expression of nestin also occurs in both pathological glial-derived tumor cells and reactive astrocytes in various CNS injuries, implying that nestin may play a crucial role in cell growth or proliferation of astrocyte-derived tumor cells. In the present study, we have investigated the possible role of nestin expression in cell growth or survival of CNS tumor cells by using novel small interfering RNA (siRNA) method in cell culture of rat astrocytoma C6 cell line. The nestin expression and cell growth of the cultured astrocytoma cells were examined after nestin siRNA duplex was delivered by cell transfection for 6 h and cell culture was maintained for 48 h. It revealed an effective suppression influence of nestin siRNA on cell growth of cultured astrocytoma cells in a dose-dependent manner. Quantitative data analysis showed that the doses of nestin siRNA at 30-120 nM significantly decreased both cell numbers and expression levels of nestin mRNA and protein. The nestin siRNA also suppressed expression of cellular glial fibrillary acid protein but showed no obvious influence on expression level of Ki-67 protein (a cell proliferation marker). This study has provided in vitro evidence that nestin siRNA can effectively block nestin expression and reduce cell growth of the cultured C6 astrocytoma cells, strongly suggesting that nestin siRNA-induced suppression of tumor cell growth may provide a potential novel clinical therapy against CNS astroglioma events. © 2007 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_HK
dc.relation.ispartofBrain Researchen_HK
dc.rightsBrain Research. Copyright © Elsevier BV.en_HK
dc.subjectAstrocytomaen_HK
dc.subjectCell cultureen_HK
dc.subjectCell growthen_HK
dc.subjectNestinen_HK
dc.subjectSmall interfering RNAen_HK
dc.titleNestin small interfering RNA (siRNA) reduces cell growth in cultured astrocytoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=1196&spage=103&epage=112&date=2008&atitle=Nestin+small+interfering+RNA+(siRNA)+reduces+cell+growth+in+cultured+astrocytoma+cells.+++en_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.brainres.2007.11.026en_HK
dc.identifier.pmid18234160-
dc.identifier.scopuseid_2-s2.0-39149088297en_HK
dc.identifier.hkuros149585en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-39149088297&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1196en_HK
dc.identifier.spage103en_HK
dc.identifier.epage112en_HK
dc.identifier.isiWOS:000254005700012-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWei, LC=7402951429en_HK
dc.identifier.scopusauthoridShi, M=36115245300en_HK
dc.identifier.scopusauthoridCao, R=34567948400en_HK
dc.identifier.scopusauthoridChen, LW=15821717100en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK

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