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Article: Melatonin and prostate cancer cell proliferation: Interplay with castration, epidermal growth factor, and androgen sensitivity

TitleMelatonin and prostate cancer cell proliferation: Interplay with castration, epidermal growth factor, and androgen sensitivity
Authors
Keywords2-iodomelatonin
Cyclin D1
DU 145
EGF
LNCaP
MT1 receptor
PC-3
Issue Date2002
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304
Citation
Prostate, 2002, v. 52 n. 2, p. 106-122 How to Cite?
AbstractBACKGROUND. Potential modulatory effects of melatonin on the proliferation of androgen-sensitive LNCaP and androgen-insensitive PC-3 and DU 145 prostate cancer cells were reported recently. In this study, we investigated the effects of combined melatonin and castration on LNCaP tumor growth in vivo, the interactions between melatonin and epidermal growth factor (EGF) on LNCaP cell proliferation, and melatonin actions on the proliferation of PC-3 and DU 145 cells. METHODS. Tumor development and growth in castrated nude mice inoculated with LNCaP cells or in intact animals inoculated with DU 145 cells, with or without daily melatonin treatment, were monitored by observation and caliper measurement. MT1 receptor expression in native or transfected prostate cancer cell lines was examined by immunocytochemistry or 2-[125I]iodomelatonin binding. Cyclin D1 expression in LNCaP cells was assessed by Western blotting, and cell proliferation was measured by thymidine incorporation and/or cell count. RESULTS. Melatonin treatment was associated with further decreases in LNCaP tumor incidence and growth rate in castrated nude mice. Melatonin and 2-iodomelatonin (a melatonin receptor agonist) attenuated EGF-stimulated increases in LNCaP cell proliferation and cyclin D1 levels. Melatonin had no effect on the proliferation or growth of MT1 receptor-expressing DU 145 cells, and of PC-3 cells in which MT1 receptor protein was undetectable. The proliferation of transfected PC-3 cells expressing MT1 receptor was unaffected by 2-iodomelatonin. CONCLUSION. Together with previous data, the present results indicate synergistic action of melatonin and castration in inhibiting the growth of androgen-sensitive LNCaP tumor. Androgen-sensitive prostate cancer cell proliferation may be modulated by opposite changes in cyclin D1 levels induced by activated MT1 and EGF receptors. In androgen-insensitive prostate cancer cells, MT1 receptor-mediated signal transduction may become defective not only through changes in membrane receptor protein expression and/or functions, but also by means of alterations in downstream postreceptor signaling events. © 2002 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/81239
ISSN
2015 Impact Factor: 3.778
2015 SCImago Journal Rankings: 1.477
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSiu, SWFen_HK
dc.contributor.authorLau, KWen_HK
dc.contributor.authorTam, PCen_HK
dc.contributor.authorShiu, SYWen_HK
dc.date.accessioned2010-09-06T08:15:24Z-
dc.date.available2010-09-06T08:15:24Z-
dc.date.issued2002en_HK
dc.identifier.citationProstate, 2002, v. 52 n. 2, p. 106-122en_HK
dc.identifier.issn0270-4137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81239-
dc.description.abstractBACKGROUND. Potential modulatory effects of melatonin on the proliferation of androgen-sensitive LNCaP and androgen-insensitive PC-3 and DU 145 prostate cancer cells were reported recently. In this study, we investigated the effects of combined melatonin and castration on LNCaP tumor growth in vivo, the interactions between melatonin and epidermal growth factor (EGF) on LNCaP cell proliferation, and melatonin actions on the proliferation of PC-3 and DU 145 cells. METHODS. Tumor development and growth in castrated nude mice inoculated with LNCaP cells or in intact animals inoculated with DU 145 cells, with or without daily melatonin treatment, were monitored by observation and caliper measurement. MT1 receptor expression in native or transfected prostate cancer cell lines was examined by immunocytochemistry or 2-[125I]iodomelatonin binding. Cyclin D1 expression in LNCaP cells was assessed by Western blotting, and cell proliferation was measured by thymidine incorporation and/or cell count. RESULTS. Melatonin treatment was associated with further decreases in LNCaP tumor incidence and growth rate in castrated nude mice. Melatonin and 2-iodomelatonin (a melatonin receptor agonist) attenuated EGF-stimulated increases in LNCaP cell proliferation and cyclin D1 levels. Melatonin had no effect on the proliferation or growth of MT1 receptor-expressing DU 145 cells, and of PC-3 cells in which MT1 receptor protein was undetectable. The proliferation of transfected PC-3 cells expressing MT1 receptor was unaffected by 2-iodomelatonin. CONCLUSION. Together with previous data, the present results indicate synergistic action of melatonin and castration in inhibiting the growth of androgen-sensitive LNCaP tumor. Androgen-sensitive prostate cancer cell proliferation may be modulated by opposite changes in cyclin D1 levels induced by activated MT1 and EGF receptors. In androgen-insensitive prostate cancer cells, MT1 receptor-mediated signal transduction may become defective not only through changes in membrane receptor protein expression and/or functions, but also by means of alterations in downstream postreceptor signaling events. © 2002 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304en_HK
dc.relation.ispartofProstateen_HK
dc.rightsThe Prostate. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject2-iodomelatoninen_HK
dc.subjectCyclin D1en_HK
dc.subjectDU 145en_HK
dc.subjectEGFen_HK
dc.subjectLNCaPen_HK
dc.subjectMT1 receptoren_HK
dc.subjectPC-3en_HK
dc.titleMelatonin and prostate cancer cell proliferation: Interplay with castration, epidermal growth factor, and androgen sensitivityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0270-4137&volume=52&spage=106&epage=122&date=2002&atitle=Melatonin+and+prostate+cancer+cell+proliferation:+interplay+with+castration,+epidermal+growth+factor,+and+androgen+sensitivityen_HK
dc.identifier.emailShiu, SYW: sywshiu@hkucc.hku.hken_HK
dc.identifier.authorityShiu, SYW=rp00384en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pros.10098en_HK
dc.identifier.pmid12111702-
dc.identifier.scopuseid_2-s2.0-0036603478en_HK
dc.identifier.hkuros81249en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036603478&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue2en_HK
dc.identifier.spage106en_HK
dc.identifier.epage122en_HK
dc.identifier.isiWOS:000176431000003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSiu, SWF=36905820000en_HK
dc.identifier.scopusauthoridLau, KW=7401560179en_HK
dc.identifier.scopusauthoridTam, PC=7202539419en_HK
dc.identifier.scopusauthoridShiu, SYW=7005550655en_HK

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