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Article: 2[125I]Iodomelatonin binding and interaction with β-adrenergic signaling in chick heart/coronary artery physiology

Title2[125I]Iodomelatonin binding and interaction with β-adrenergic signaling in chick heart/coronary artery physiology
Authors
KeywordsAnti-MT1
Autoradiography
CAMP
Dopamine
Embryo
Isoproterenol
Pineal
Issue Date2002
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI
Citation
Journal Of Pineal Research, 2002, v. 32 n. 4, p. 243-252 How to Cite?
Abstract2[125I]Iodomelatonin ([125I]Mel) binding sites were characterized on membrane preparations of young chick hearts. [125I]Mel binding was rapid, saturable, stable, reversible, specific and of picomolar affinity and femtomolar density. Guanosine 5′-O-(3-thiotriphosphate) significantly lowered the binding affinity by one- to twofold, supporting G-protein linkage of melatonin receptors. Binding was detected as early as embryonic day-9 (E9), and increased steadily peaking at E13 before it slowly declined to about 15% of the peak level a week posthatch. Specific [125I]Mel binding was significantly increased by in ovo administration of inotropic agents dopamine and isoproterenol. Melatonin or 2-iodo-N-butanoyl-tryptamine inhibited isoproterenol-stimulated cAMP accumulation in primary heart cell cultures and the effect was attenuated after pretreatment with pertussis toxin (PTX). Localization of melatonin receptors using autoradiography showed intense labeling in the coronary arteries in all age groups whereas those in the myoblasts decreased as the heart matured. While the myoblasts and undifferentiated developing coronary arteries expressed melatonin MT1 receptor subtype in E11 hearts as detected by immunostaining with anti-MT1 receptor serum, immunoreactivities were observed mostly on the endothelium/subendothelium and smooth muscle cells of the well developed coronary vessels in posthatch hearts. Collectively, our data suggest the presence of PTX-sensitive, G protein-coupled melatonin receptors, whose expression is up-regulated by dopamine and isoproterenol, in the chick heart. Activation of these receptors, which include MT1 subtype, may modulate β-adrenergic receptor-mediated cAMP signaling in the control of chick heart and coronary artery physiology.
Persistent Identifierhttp://hdl.handle.net/10722/81237
ISSN
2015 Impact Factor: 9.314
2015 SCImago Journal Rankings: 2.655
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPang, CSen_HK
dc.contributor.authorXi, SCen_HK
dc.contributor.authorBrown, GMen_HK
dc.contributor.authorPang, SFen_HK
dc.contributor.authorShiu, SYWen_HK
dc.date.accessioned2010-09-06T08:15:22Z-
dc.date.available2010-09-06T08:15:22Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Pineal Research, 2002, v. 32 n. 4, p. 243-252en_HK
dc.identifier.issn0742-3098en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81237-
dc.description.abstract2[125I]Iodomelatonin ([125I]Mel) binding sites were characterized on membrane preparations of young chick hearts. [125I]Mel binding was rapid, saturable, stable, reversible, specific and of picomolar affinity and femtomolar density. Guanosine 5′-O-(3-thiotriphosphate) significantly lowered the binding affinity by one- to twofold, supporting G-protein linkage of melatonin receptors. Binding was detected as early as embryonic day-9 (E9), and increased steadily peaking at E13 before it slowly declined to about 15% of the peak level a week posthatch. Specific [125I]Mel binding was significantly increased by in ovo administration of inotropic agents dopamine and isoproterenol. Melatonin or 2-iodo-N-butanoyl-tryptamine inhibited isoproterenol-stimulated cAMP accumulation in primary heart cell cultures and the effect was attenuated after pretreatment with pertussis toxin (PTX). Localization of melatonin receptors using autoradiography showed intense labeling in the coronary arteries in all age groups whereas those in the myoblasts decreased as the heart matured. While the myoblasts and undifferentiated developing coronary arteries expressed melatonin MT1 receptor subtype in E11 hearts as detected by immunostaining with anti-MT1 receptor serum, immunoreactivities were observed mostly on the endothelium/subendothelium and smooth muscle cells of the well developed coronary vessels in posthatch hearts. Collectively, our data suggest the presence of PTX-sensitive, G protein-coupled melatonin receptors, whose expression is up-regulated by dopamine and isoproterenol, in the chick heart. Activation of these receptors, which include MT1 subtype, may modulate β-adrenergic receptor-mediated cAMP signaling in the control of chick heart and coronary artery physiology.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPIen_HK
dc.relation.ispartofJournal of Pineal Researchen_HK
dc.subjectAnti-MT1en_HK
dc.subjectAutoradiographyen_HK
dc.subjectCAMPen_HK
dc.subjectDopamineen_HK
dc.subjectEmbryoen_HK
dc.subjectIsoproterenolen_HK
dc.subjectPinealen_HK
dc.title2[125I]Iodomelatonin binding and interaction with β-adrenergic signaling in chick heart/coronary artery physiologyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0742-3098&volume=32&spage=243&epage=252&date=2002&atitle=2[125I]Iodomelatonin+binding+and+interaction+with+beta-adrenergic+signaling+in+chick+heart/coronary+artery+physiologyen_HK
dc.identifier.emailShiu, SYW: sywshiu@hkucc.hku.hken_HK
dc.identifier.authorityShiu, SYW=rp00384en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1034/j.1600-079X.2002.01860.xen_HK
dc.identifier.pmid11982794-
dc.identifier.scopuseid_2-s2.0-0035986042en_HK
dc.identifier.hkuros72048en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035986042&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue4en_HK
dc.identifier.spage243en_HK
dc.identifier.epage252en_HK
dc.identifier.isiWOS:000175889300007-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridPang, CS=7201425191en_HK
dc.identifier.scopusauthoridXi, SC=35944696100en_HK
dc.identifier.scopusauthoridBrown, GM=35493704500en_HK
dc.identifier.scopusauthoridPang, SF=7402528719en_HK
dc.identifier.scopusauthoridShiu, SYW=7005550655en_HK

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