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Article: Functional expression of angiotensin II receptors in type-I cells of the rat carotid body

TitleFunctional expression of angiotensin II receptors in type-I cells of the rat carotid body
Authors
KeywordsAngiotensin II
Angiotensin II receptor
AT 1a
AT 1b
Carotid body
Glomus cell
Respiration
Type-I cell
Issue Date2001
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00424/index.htm
Citation
Pflugers Archiv European Journal Of Physiology, 2001, v. 441 n. 4, p. 474-480 How to Cite?
AbstractAngiotensin II increases afferent discharge from the carotid body in vitro. We hypothesized that angiotensin II receptors (AT receptors) are expressed functionally in the type-I cell of the carotid body. Cytosolic free [Ca 2+] ([Ca 2+] i) in type-I cells freshly dissociated from rat carotid bodies was measured spectrofluorimetrically. Angiotensin II (10-100 nM) concentration-dependently increased [Ca 2+] i in type-I cells. The [Ca 2+] i response was blocked by pretreatment with losartan (1 μM), an AT 1 receptor antagonist, but not by blockade of AT 2 receptors with PD-123319 (1 μM). Moreover, the gene expression of AT 1 receptors was assessed by the reverse transcriptase polymerase chain reaction and gene transcripts of both AT 1a and AT 1b receptors were detected in the carotid body. In addition, immunohistochemical study revealed that AT 1 immunoreactivity was localized in lobules of type-I cells in the carotid body. Taken together, these results suggest that type-I cells in the rat carotid body express functional angiotensin II receptors. The binding of angiotensin II to the AT 1 receptors increases [Ca 2+] i, a key step of the intracellular signalling cascade following the activation of the receptors. It is concluded that angiotensin II modulates carotid body chemoreceptor function directly via AT 1 receptors in the type-I cell.
Persistent Identifierhttp://hdl.handle.net/10722/81217
ISSN
2015 Impact Factor: 3.654
2015 SCImago Journal Rankings: 1.638
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFung, MLen_HK
dc.contributor.authorLam, SYen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorDong, Xen_HK
dc.contributor.authorLeung, PSen_HK
dc.date.accessioned2010-09-06T08:15:09Z-
dc.date.available2010-09-06T08:15:09Z-
dc.date.issued2001en_HK
dc.identifier.citationPflugers Archiv European Journal Of Physiology, 2001, v. 441 n. 4, p. 474-480en_HK
dc.identifier.issn0031-6768en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81217-
dc.description.abstractAngiotensin II increases afferent discharge from the carotid body in vitro. We hypothesized that angiotensin II receptors (AT receptors) are expressed functionally in the type-I cell of the carotid body. Cytosolic free [Ca 2+] ([Ca 2+] i) in type-I cells freshly dissociated from rat carotid bodies was measured spectrofluorimetrically. Angiotensin II (10-100 nM) concentration-dependently increased [Ca 2+] i in type-I cells. The [Ca 2+] i response was blocked by pretreatment with losartan (1 μM), an AT 1 receptor antagonist, but not by blockade of AT 2 receptors with PD-123319 (1 μM). Moreover, the gene expression of AT 1 receptors was assessed by the reverse transcriptase polymerase chain reaction and gene transcripts of both AT 1a and AT 1b receptors were detected in the carotid body. In addition, immunohistochemical study revealed that AT 1 immunoreactivity was localized in lobules of type-I cells in the carotid body. Taken together, these results suggest that type-I cells in the rat carotid body express functional angiotensin II receptors. The binding of angiotensin II to the AT 1 receptors increases [Ca 2+] i, a key step of the intracellular signalling cascade following the activation of the receptors. It is concluded that angiotensin II modulates carotid body chemoreceptor function directly via AT 1 receptors in the type-I cell.en_HK
dc.languageengen_HK
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00424/index.htmen_HK
dc.relation.ispartofPflugers Archiv European Journal of Physiologyen_HK
dc.subjectAngiotensin IIen_HK
dc.subjectAngiotensin II receptoren_HK
dc.subjectAT 1aen_HK
dc.subjectAT 1ben_HK
dc.subjectCarotid bodyen_HK
dc.subjectGlomus cellen_HK
dc.subjectRespirationen_HK
dc.subjectType-I cellen_HK
dc.titleFunctional expression of angiotensin II receptors in type-I cells of the rat carotid bodyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0031-6768&volume=441&spage=474&epage=480&date=2000&atitle=Functional+expression+of+angiotensin+II+receptors+in+type-I+cells+of+the+rat+carotid+bodyen_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s004240000445en_HK
dc.identifier.pmid11212210-
dc.identifier.scopuseid_2-s2.0-0035144374en_HK
dc.identifier.hkuros58108en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035144374&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume441en_HK
dc.identifier.issue4en_HK
dc.identifier.spage474en_HK
dc.identifier.epage480en_HK
dc.identifier.isiWOS:000166485100008-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridFung, ML=7101955092en_HK
dc.identifier.scopusauthoridLam, SY=7402279518en_HK
dc.identifier.scopusauthoridChen, Y=7601439932en_HK
dc.identifier.scopusauthoridDong, X=55184171400en_HK
dc.identifier.scopusauthoridLeung, PS=7401748938en_HK

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