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Article: mt1 receptor-mediated antiproliferative effects of melatonin on the rat uterine antimesometrial stromal cells

Titlemt1 receptor-mediated antiproliferative effects of melatonin on the rat uterine antimesometrial stromal cells
Authors
KeywordsCamp
Female reproductive system
Forskolin
Pineal glan
Issue Date2002
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692
Citation
Molecular Reproduction And Development, 2002, v. 61 n. 2, p. 192-199 How to Cite?
AbstractIt has been shown that melatonin regulates uterine function. Our previous studies have demonstrated the presence of melatonin receptors in the rat uterine endometrium, indicating that melatonin may act directly on the uterus. In the present study, the histological localization of the rat uterine melatonin binding was revealed by autoradiography and the molecular subtyping was studied by in situ hybridization in the stromal cells. The signal transduction process and effects of melatonin on stromal cell proliferation was also investigated. Our autoradiograms showed that 2[125l]iodomelatonin binding sites were localized in the antimesometrial endometrial stroma. In situ hybridization with specific mt1 receptor cdna probe in the primary culture of antimesometrial stromal cells demonstrated the expression of mt1 receptor mrnas. Melatonin dose-dependently inhibited forskolin-stimulated camp accumulation, which was reversed by pertussis toxin. This indicates that the rat uterine melatonin receptors are negatively coupled to adenylate cyclase via pertussis toxin sensitive Gi protein. Melatonin also inhibited the incorporation of [3H]thymidine in the rat uterine antimesometrial stromal cells, showing that melatonin has an antiproliferative effect on the uterus. Our results suggest that melatonin may act directly on the mt1 melatonin receptors in the rat uterine antimesometrial stromal cells to inhibit their proliferation. Its action may be mediated through a pertussis toxin-sensitive adenylate cyclase coupled Gi-protein. © 2002 Wiley-liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/81208
ISSN
2015 Impact Factor: 2.141
2015 SCImago Journal Rankings: 1.041
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhao, Hen_HK
dc.contributor.authorPang, SFen_HK
dc.contributor.authorPoon, AMSen_HK
dc.date.accessioned2010-09-06T08:15:03Z-
dc.date.available2010-09-06T08:15:03Z-
dc.date.issued2002en_HK
dc.identifier.citationMolecular Reproduction And Development, 2002, v. 61 n. 2, p. 192-199en_HK
dc.identifier.issn1040-452Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/81208-
dc.description.abstractIt has been shown that melatonin regulates uterine function. Our previous studies have demonstrated the presence of melatonin receptors in the rat uterine endometrium, indicating that melatonin may act directly on the uterus. In the present study, the histological localization of the rat uterine melatonin binding was revealed by autoradiography and the molecular subtyping was studied by in situ hybridization in the stromal cells. The signal transduction process and effects of melatonin on stromal cell proliferation was also investigated. Our autoradiograms showed that 2[125l]iodomelatonin binding sites were localized in the antimesometrial endometrial stroma. In situ hybridization with specific mt1 receptor cdna probe in the primary culture of antimesometrial stromal cells demonstrated the expression of mt1 receptor mrnas. Melatonin dose-dependently inhibited forskolin-stimulated camp accumulation, which was reversed by pertussis toxin. This indicates that the rat uterine melatonin receptors are negatively coupled to adenylate cyclase via pertussis toxin sensitive Gi protein. Melatonin also inhibited the incorporation of [3H]thymidine in the rat uterine antimesometrial stromal cells, showing that melatonin has an antiproliferative effect on the uterus. Our results suggest that melatonin may act directly on the mt1 melatonin receptors in the rat uterine antimesometrial stromal cells to inhibit their proliferation. Its action may be mediated through a pertussis toxin-sensitive adenylate cyclase coupled Gi-protein. © 2002 Wiley-liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692en_HK
dc.relation.ispartofMolecular Reproduction and Developmenten_HK
dc.subjectCampen_HK
dc.subjectFemale reproductive systemen_HK
dc.subjectForskolinen_HK
dc.subjectPineal glanen_HK
dc.titlemt1 receptor-mediated antiproliferative effects of melatonin on the rat uterine antimesometrial stromal cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailPoon, AMS: amspoon@hkucc.hku.hken_HK
dc.identifier.authorityPoon, AMS=rp00354en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mrd.1147en_HK
dc.identifier.pmid11803554-
dc.identifier.scopuseid_2-s2.0-0036138989en_HK
dc.identifier.hkuros72789en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036138989&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume61en_HK
dc.identifier.issue2en_HK
dc.identifier.spage192en_HK
dc.identifier.epage199en_HK
dc.identifier.isiWOS:000173100800008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhao, H=14821083400en_HK
dc.identifier.scopusauthoridPang, SF=7402528719en_HK
dc.identifier.scopusauthoridPoon, AMS=7103068868en_HK

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