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Article: Chronic hypoxia induced down-regulation of angiotensinogen expression in rat epididymis

TitleChronic hypoxia induced down-regulation of angiotensinogen expression in rat epididymis
Authors
KeywordsAngiotensinogen
Chronic hypoxia
Epididymis
Renin-angiotensin system
Issue Date2001
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/regpep
Citation
Regulatory Peptides, 2001, v. 96 n. 3, p. 143-149 How to Cite?
AbstractThe presence of an intrinsic renin-angiotensin system (RAS) in the rat epididymis has been previously established by showing the expression of several key RAS components, and in particular angiotensinogen, the indispensable element for the intracellular generation of angiotensin II. In this study, the possible involvement of this local epididymal RAS in the testicular effects of chronic hypoxia was investigated. Semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR), Western blotting and by in situ hybridization histochemistry of the rat epididymis were used to show changes in localization and expression of angiotensinogen. Results from RT-PCR analysis demonstrated that chronic hypoxia caused a marked decrease (60%) in the expression of angiotensinogen mRNA, when compared with that in the normoxic epididymis. Western blot analysis demonstrated a less decrease (35%) in the expression of angiotensinogen protein. In situ hybridization histochemistry showed that the reduced angiotensinogen mRNA in chronic hypoxia was specifically localized to the epididymal epithelium from the cauda, corpus and caput regions of the epididymis; a distribution similar to that of normoxic rats. It was concluded that chronic hypoxia decreases the transcriptional and translational expression of angiotensinogen, and thus local formation of angiotensin II, in the rat epididymis. Copyright (C) 2001 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/81199
ISSN
2015 Impact Factor: 1.813
2015 SCImago Journal Rankings: 0.915
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, PSen_HK
dc.contributor.authorFung, MLen_HK
dc.contributor.authorSernia, Cen_HK
dc.date.accessioned2010-09-06T08:14:57Z-
dc.date.available2010-09-06T08:14:57Z-
dc.date.issued2001en_HK
dc.identifier.citationRegulatory Peptides, 2001, v. 96 n. 3, p. 143-149en_HK
dc.identifier.issn0167-0115en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81199-
dc.description.abstractThe presence of an intrinsic renin-angiotensin system (RAS) in the rat epididymis has been previously established by showing the expression of several key RAS components, and in particular angiotensinogen, the indispensable element for the intracellular generation of angiotensin II. In this study, the possible involvement of this local epididymal RAS in the testicular effects of chronic hypoxia was investigated. Semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR), Western blotting and by in situ hybridization histochemistry of the rat epididymis were used to show changes in localization and expression of angiotensinogen. Results from RT-PCR analysis demonstrated that chronic hypoxia caused a marked decrease (60%) in the expression of angiotensinogen mRNA, when compared with that in the normoxic epididymis. Western blot analysis demonstrated a less decrease (35%) in the expression of angiotensinogen protein. In situ hybridization histochemistry showed that the reduced angiotensinogen mRNA in chronic hypoxia was specifically localized to the epididymal epithelium from the cauda, corpus and caput regions of the epididymis; a distribution similar to that of normoxic rats. It was concluded that chronic hypoxia decreases the transcriptional and translational expression of angiotensinogen, and thus local formation of angiotensin II, in the rat epididymis. Copyright (C) 2001 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/regpepen_HK
dc.relation.ispartofRegulatory Peptidesen_HK
dc.rightsRegulatory Peptides. Copyright © Elsevier BV.en_HK
dc.subjectAngiotensinogenen_HK
dc.subjectChronic hypoxiaen_HK
dc.subjectEpididymisen_HK
dc.subjectRenin-angiotensin systemen_HK
dc.titleChronic hypoxia induced down-regulation of angiotensinogen expression in rat epididymisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-0115&volume=96&issue=3&spage=143&epage=149&date=2001&atitle=Chronic+hypoxia+induced+down-regulation+of+angiotensinogen+expression+in+rat+epididymisen_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0167-0115(00)00169-5en_HK
dc.identifier.pmid11111020-
dc.identifier.scopuseid_2-s2.0-0035847296en_HK
dc.identifier.hkuros58096en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035847296&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume96en_HK
dc.identifier.issue3en_HK
dc.identifier.spage143en_HK
dc.identifier.epage149en_HK
dc.identifier.isiWOS:000166373900007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLeung, PS=7401748938en_HK
dc.identifier.scopusauthoridFung, ML=7101955092en_HK
dc.identifier.scopusauthoridSernia, C=7005069762en_HK

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