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- Publisher Website: 10.1111/j.1600-079X.1998.tb00391.x
- Scopus: eid_2-s2.0-0032429941
- PMID: 9885991
- WOS: WOS:000077968000004
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Article: Molecular and cellular analyses of melatonin receptor-mediated cAMP signaling in rat corpus epididymis
Title | Molecular and cellular analyses of melatonin receptor-mediated cAMP signaling in rat corpus epididymis |
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Authors | |
Keywords | 5α- dihydrotestosterone Corpus epididymis Epithelial cells G protein Isoproterenol Melatonin receptor Norepinephrine |
Issue Date | 1998 |
Publisher | Blackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI |
Citation | Journal Of Pineal Research, 1998, v. 25 n. 4, p. 219-228 How to Cite? |
Abstract | By using 2-[ 125I]iodomelatonin receptor binding studies, we have previously demonstrated high affinity melatonin receptors, the binding activities of which are regulated by testosterone, in the corpus epididymis of rats. In this report, some of the basic molecular and cellular characteristics of these high affinity melatonin receptors in rat corpus epididymis were analyzed. MEL(1A) and MEL(1B) receptor mRNAs were expressed by rat corpus epididymal epithelial cells as revealed by in situ hybridization. Functionally, these high affinity melatonin receptors are negatively coupled to adenylyl cyclase via pertussis toxin (PTX) sensitive G(i) protein and the inhibitory effects of melatonin on forskolin-stimulated cAMP accumulation were enhanced by 5α-dihydrotestosterone (5α-DHT). Interestingly, opposing interactions between melatonin and β-adrenergic receptor signaling in rat epididymal epithelial cells were observed with melatonin inhibiting norepinephrine- and isoproterenol-stimulated cAMP accumulation. In conclusion, our data support a modulatory action of melatonin, mediated via pertussis toxin-sensitive G(i)-coupled MEL(1A) and MEL(1B) receptors, in androgenic and adrenergic regulation of rat corpus epididymal epithelial cell functions. |
Persistent Identifier | http://hdl.handle.net/10722/81173 |
ISSN | 2023 Impact Factor: 8.3 2023 SCImago Journal Rankings: 2.194 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, L | en_HK |
dc.contributor.author | Xu, JN | en_HK |
dc.contributor.author | Wong, YH | en_HK |
dc.contributor.author | Wong, JTY | en_HK |
dc.contributor.author | Pang, SF | en_HK |
dc.contributor.author | Shiu, SYW | en_HK |
dc.date.accessioned | 2010-09-06T08:14:39Z | - |
dc.date.available | 2010-09-06T08:14:39Z | - |
dc.date.issued | 1998 | en_HK |
dc.identifier.citation | Journal Of Pineal Research, 1998, v. 25 n. 4, p. 219-228 | en_HK |
dc.identifier.issn | 0742-3098 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/81173 | - |
dc.description.abstract | By using 2-[ 125I]iodomelatonin receptor binding studies, we have previously demonstrated high affinity melatonin receptors, the binding activities of which are regulated by testosterone, in the corpus epididymis of rats. In this report, some of the basic molecular and cellular characteristics of these high affinity melatonin receptors in rat corpus epididymis were analyzed. MEL(1A) and MEL(1B) receptor mRNAs were expressed by rat corpus epididymal epithelial cells as revealed by in situ hybridization. Functionally, these high affinity melatonin receptors are negatively coupled to adenylyl cyclase via pertussis toxin (PTX) sensitive G(i) protein and the inhibitory effects of melatonin on forskolin-stimulated cAMP accumulation were enhanced by 5α-dihydrotestosterone (5α-DHT). Interestingly, opposing interactions between melatonin and β-adrenergic receptor signaling in rat epididymal epithelial cells were observed with melatonin inhibiting norepinephrine- and isoproterenol-stimulated cAMP accumulation. In conclusion, our data support a modulatory action of melatonin, mediated via pertussis toxin-sensitive G(i)-coupled MEL(1A) and MEL(1B) receptors, in androgenic and adrenergic regulation of rat corpus epididymal epithelial cell functions. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Blackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI | en_HK |
dc.relation.ispartof | Journal of Pineal Research | en_HK |
dc.subject | 5α- dihydrotestosterone | en_HK |
dc.subject | Corpus epididymis | en_HK |
dc.subject | Epithelial cells | en_HK |
dc.subject | G protein | en_HK |
dc.subject | Isoproterenol | en_HK |
dc.subject | Melatonin receptor | en_HK |
dc.subject | Norepinephrine | en_HK |
dc.title | Molecular and cellular analyses of melatonin receptor-mediated cAMP signaling in rat corpus epididymis | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0742-3098&volume=25&spage=219&epage=228&date=1998&atitle=Molecular+and+cellular+analyses+of+melatonin+receptor-mediated+cAMP+signaling+in+rat+corpus+epididymis | en_HK |
dc.identifier.email | Shiu, SYW: sywshiu@hkucc.hku.hk | en_HK |
dc.identifier.authority | Shiu, SYW=rp00384 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1111/j.1600-079X.1998.tb00391.x | en_HK |
dc.identifier.pmid | 9885991 | - |
dc.identifier.scopus | eid_2-s2.0-0032429941 | en_HK |
dc.identifier.hkuros | 43589 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032429941&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 25 | en_HK |
dc.identifier.issue | 4 | en_HK |
dc.identifier.spage | 219 | en_HK |
dc.identifier.epage | 228 | en_HK |
dc.identifier.isi | WOS:000077968000004 | - |
dc.publisher.place | Denmark | en_HK |
dc.identifier.scopusauthorid | Li, L=36985969000 | en_HK |
dc.identifier.scopusauthorid | Xu, JN=7407009328 | en_HK |
dc.identifier.scopusauthorid | Wong, YH=35263245300 | en_HK |
dc.identifier.scopusauthorid | Wong, JTY=24467064200 | en_HK |
dc.identifier.scopusauthorid | Pang, SF=7402528719 | en_HK |
dc.identifier.scopusauthorid | Shiu, SYW=7005550655 | en_HK |
dc.identifier.issnl | 0742-3098 | - |