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Article: Molecular and cellular analyses of melatonin receptor-mediated cAMP signaling in rat corpus epididymis

TitleMolecular and cellular analyses of melatonin receptor-mediated cAMP signaling in rat corpus epididymis
Authors
Keywords5α- dihydrotestosterone
Corpus epididymis
Epithelial cells
G protein
Isoproterenol
Melatonin receptor
Norepinephrine
Issue Date1998
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI
Citation
Journal Of Pineal Research, 1998, v. 25 n. 4, p. 219-228 How to Cite?
AbstractBy using 2-[ 125I]iodomelatonin receptor binding studies, we have previously demonstrated high affinity melatonin receptors, the binding activities of which are regulated by testosterone, in the corpus epididymis of rats. In this report, some of the basic molecular and cellular characteristics of these high affinity melatonin receptors in rat corpus epididymis were analyzed. MEL(1A) and MEL(1B) receptor mRNAs were expressed by rat corpus epididymal epithelial cells as revealed by in situ hybridization. Functionally, these high affinity melatonin receptors are negatively coupled to adenylyl cyclase via pertussis toxin (PTX) sensitive G(i) protein and the inhibitory effects of melatonin on forskolin-stimulated cAMP accumulation were enhanced by 5α-dihydrotestosterone (5α-DHT). Interestingly, opposing interactions between melatonin and β-adrenergic receptor signaling in rat epididymal epithelial cells were observed with melatonin inhibiting norepinephrine- and isoproterenol-stimulated cAMP accumulation. In conclusion, our data support a modulatory action of melatonin, mediated via pertussis toxin-sensitive G(i)-coupled MEL(1A) and MEL(1B) receptors, in androgenic and adrenergic regulation of rat corpus epididymal epithelial cell functions.
Persistent Identifierhttp://hdl.handle.net/10722/81173
ISSN
2015 Impact Factor: 9.314
2015 SCImago Journal Rankings: 2.655
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Len_HK
dc.contributor.authorXu, JNen_HK
dc.contributor.authorWong, YHen_HK
dc.contributor.authorWong, JTYen_HK
dc.contributor.authorPang, SFen_HK
dc.contributor.authorShiu, SYWen_HK
dc.date.accessioned2010-09-06T08:14:39Z-
dc.date.available2010-09-06T08:14:39Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Pineal Research, 1998, v. 25 n. 4, p. 219-228en_HK
dc.identifier.issn0742-3098en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81173-
dc.description.abstractBy using 2-[ 125I]iodomelatonin receptor binding studies, we have previously demonstrated high affinity melatonin receptors, the binding activities of which are regulated by testosterone, in the corpus epididymis of rats. In this report, some of the basic molecular and cellular characteristics of these high affinity melatonin receptors in rat corpus epididymis were analyzed. MEL(1A) and MEL(1B) receptor mRNAs were expressed by rat corpus epididymal epithelial cells as revealed by in situ hybridization. Functionally, these high affinity melatonin receptors are negatively coupled to adenylyl cyclase via pertussis toxin (PTX) sensitive G(i) protein and the inhibitory effects of melatonin on forskolin-stimulated cAMP accumulation were enhanced by 5α-dihydrotestosterone (5α-DHT). Interestingly, opposing interactions between melatonin and β-adrenergic receptor signaling in rat epididymal epithelial cells were observed with melatonin inhibiting norepinephrine- and isoproterenol-stimulated cAMP accumulation. In conclusion, our data support a modulatory action of melatonin, mediated via pertussis toxin-sensitive G(i)-coupled MEL(1A) and MEL(1B) receptors, in androgenic and adrenergic regulation of rat corpus epididymal epithelial cell functions.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPIen_HK
dc.relation.ispartofJournal of Pineal Researchen_HK
dc.subject5α- dihydrotestosteroneen_HK
dc.subjectCorpus epididymisen_HK
dc.subjectEpithelial cellsen_HK
dc.subjectG proteinen_HK
dc.subjectIsoproterenolen_HK
dc.subjectMelatonin receptoren_HK
dc.subjectNorepinephrineen_HK
dc.titleMolecular and cellular analyses of melatonin receptor-mediated cAMP signaling in rat corpus epididymisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0742-3098&volume=25&spage=219&epage=228&date=1998&atitle=Molecular+and+cellular+analyses+of+melatonin+receptor-mediated+cAMP+signaling+in+rat+corpus+epididymisen_HK
dc.identifier.emailShiu, SYW: sywshiu@hkucc.hku.hken_HK
dc.identifier.authorityShiu, SYW=rp00384en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-079X.1998.tb00391.xen_HK
dc.identifier.pmid9885991-
dc.identifier.scopuseid_2-s2.0-0032429941en_HK
dc.identifier.hkuros43589en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032429941&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue4en_HK
dc.identifier.spage219en_HK
dc.identifier.epage228en_HK
dc.identifier.isiWOS:000077968000004-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridLi, L=36985969000en_HK
dc.identifier.scopusauthoridXu, JN=7407009328en_HK
dc.identifier.scopusauthoridWong, YH=35263245300en_HK
dc.identifier.scopusauthoridWong, JTY=24467064200en_HK
dc.identifier.scopusauthoridPang, SF=7402528719en_HK
dc.identifier.scopusauthoridShiu, SYW=7005550655en_HK

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