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Article: Modulation of 2[125I]iodomelatonin binding sites in the guinea pig spleen by melatonin injection is dependent on the dose and period but not the time

TitleModulation of 2[125I]iodomelatonin binding sites in the guinea pig spleen by melatonin injection is dependent on the dose and period but not the time
Authors
Issue Date1994
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1994, v. 54 n. 19, p. 1441-1448 How to Cite?
AbstractEffects of dose, time and period of melatonin injection on 2[125I]iodomelatonin binding sites in the guinea pig spleen were studied. Guinea pigs (Dunkin-hartley), kept under 12h light/12 h darkness, were given daily intraperitoneal injections of either vehicle or 0.01, 0.1 or 1 mg melatonin/kg body weight in either early (1 hour after onset of light period) or late light period (1 hour before offset of light period) for 2 or 7 days. To study the effect of opioid antagonist on the binding, intraperitoneal injections of 2 or 20 mg naltrexone/kg body weight alone or together with 0.1 mg melatonin/kg body weight was given daily in late light period of 2 days. 2[125I]Iodomelatonin binding assays were performed on spleen membrane preparations and radioimmunoassays of melatonin levels were carried out in serum and pineal glands collected during mid-light. High dose (1 mg/kg body weight) of melatonin injection elevated the mid-light serum melatonin levels without affecting pineal melatonin levels. Early light injection group had a higher mid-light serum melatonin level. Melatonin injection for 2 days at either time points caused a dose-dependent decrease in Bmax and increase in Kd of 2[125I]iodomelatonin binding sites in the spleen. The response was independent of the time of injection. A greater suppression of binding was achieved by injecting melatonin for 7 days. Naltrexone did not affect the binding by itself and was not able to reverse the melatonin-induced suppression of binding in the spleen. The modulation of the splenic 2[125I]iodomelatonin binding sites by exogenous melatonin suggests that melatonin may act directly on the immune system to affect its function.
Persistent Identifierhttp://hdl.handle.net/10722/81164
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPoon, AMSen_HK
dc.contributor.authorPang, SFen_HK
dc.date.accessioned2010-09-06T08:14:33Z-
dc.date.available2010-09-06T08:14:33Z-
dc.date.issued1994en_HK
dc.identifier.citationLife Sciences, 1994, v. 54 n. 19, p. 1441-1448en_HK
dc.identifier.issn0024-3205en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81164-
dc.description.abstractEffects of dose, time and period of melatonin injection on 2[125I]iodomelatonin binding sites in the guinea pig spleen were studied. Guinea pigs (Dunkin-hartley), kept under 12h light/12 h darkness, were given daily intraperitoneal injections of either vehicle or 0.01, 0.1 or 1 mg melatonin/kg body weight in either early (1 hour after onset of light period) or late light period (1 hour before offset of light period) for 2 or 7 days. To study the effect of opioid antagonist on the binding, intraperitoneal injections of 2 or 20 mg naltrexone/kg body weight alone or together with 0.1 mg melatonin/kg body weight was given daily in late light period of 2 days. 2[125I]Iodomelatonin binding assays were performed on spleen membrane preparations and radioimmunoassays of melatonin levels were carried out in serum and pineal glands collected during mid-light. High dose (1 mg/kg body weight) of melatonin injection elevated the mid-light serum melatonin levels without affecting pineal melatonin levels. Early light injection group had a higher mid-light serum melatonin level. Melatonin injection for 2 days at either time points caused a dose-dependent decrease in Bmax and increase in Kd of 2[125I]iodomelatonin binding sites in the spleen. The response was independent of the time of injection. A greater suppression of binding was achieved by injecting melatonin for 7 days. Naltrexone did not affect the binding by itself and was not able to reverse the melatonin-induced suppression of binding in the spleen. The modulation of the splenic 2[125I]iodomelatonin binding sites by exogenous melatonin suggests that melatonin may act directly on the immune system to affect its function.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_HK
dc.relation.ispartofLife Sciencesen_HK
dc.rightsLife Sciences. Copyright © Elsevier Inc.en_HK
dc.titleModulation of 2[125I]iodomelatonin binding sites in the guinea pig spleen by melatonin injection is dependent on the dose and period but not the timeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0024-3205&volume=54&spage=1441&epage=1448&date=1994&atitle=Modulation+of+2+125I+iodomelatonin+binding+sites+in+the+guinea+pig+spleen+by+melatonin+injection+is+dependent+on+the+dose+and+period+but+not+the+time.en_HK
dc.identifier.emailPoon, AMS: amspoon@hkucc.hku.hken_HK
dc.identifier.authorityPoon, AMS=rp00354en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8190017-
dc.identifier.scopuseid_2-s2.0-0028178863en_HK
dc.identifier.hkuros2688en_HK
dc.identifier.volume54en_HK
dc.identifier.issue19en_HK
dc.identifier.spage1441en_HK
dc.identifier.epage1448en_HK
dc.identifier.isiWOS:A1994ND19800007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridPoon, AMS=7103068868en_HK
dc.identifier.scopusauthoridPang, SF=7402528719en_HK

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