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Article: Pharmacological characterization, molecular subtyping, and autoradiographic localization of putative melatonin receptors in uterine endometrium of estrous rats

TitlePharmacological characterization, molecular subtyping, and autoradiographic localization of putative melatonin receptors in uterine endometrium of estrous rats
Authors
Keywords2-iodomelatonin
Female reproductive system
N- acetylserotonin
Pineal gland
Issue Date2000
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2000, v. 66 n. 17, p. 1581-1591 How to Cite?
AbstractThe objective of this study was to determine the biochemical characteristics, subtypes, and localization of melatonin receptors in the rat uterus in estrous stage. Autoradiography with the melatonin ligand, 2- [125I]iodomelatonin, showed that melatonin receptors were localized in the rat uterine endometrium. Binding of 2[125I]iodomelatonin in crude membrane preparations of rat uterine endometrium in estrous stage was stable, saturable, reversible and of high affinity. Rosenthal analysis yielded an equilibrium dissociation constant (Kd) of 28.9 ± 3.59 pmol/l (n = 8) and a maximum number of binding sites (Bmax) of 1.6 ± 0.15 fmol/mg protein (n = 8). The Kd value determined from kinetic analysis was 16.5 ± 3.02 pmol/l (n = 3). Competition studies using various indoles and neurotransmitters demonstrated that 2-iodomelatonin, melatonin, 6-chloromelatonin, 6- hydroxymelatonin and N-acetylserotonin showed significant inhibition of the 2-[125I]iodomelatonin binding, while the other indole compounds tested had no significant inhibition. The expression of rat uterine endometrial melatonin receptor subtypes was studied by reverse transcription-polymerase chain reaction (RT-PCR) using mt1 and MT2 receptor gene-specific primers. mt1 receptor cDNA was amplified and confirmed by nucleotide sequencing. These findings indicate that mt1 receptors were present in the rat uterine endometrium, and suggest that melatonin plays an integral part in uterine physiology.
Persistent Identifierhttp://hdl.handle.net/10722/81157
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhao, Hen_HK
dc.contributor.authorPoon, AMSen_HK
dc.contributor.authorPang, SFen_HK
dc.date.accessioned2010-09-06T08:14:29Z-
dc.date.available2010-09-06T08:14:29Z-
dc.date.issued2000en_HK
dc.identifier.citationLife Sciences, 2000, v. 66 n. 17, p. 1581-1591en_HK
dc.identifier.issn0024-3205en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81157-
dc.description.abstractThe objective of this study was to determine the biochemical characteristics, subtypes, and localization of melatonin receptors in the rat uterus in estrous stage. Autoradiography with the melatonin ligand, 2- [125I]iodomelatonin, showed that melatonin receptors were localized in the rat uterine endometrium. Binding of 2[125I]iodomelatonin in crude membrane preparations of rat uterine endometrium in estrous stage was stable, saturable, reversible and of high affinity. Rosenthal analysis yielded an equilibrium dissociation constant (Kd) of 28.9 ± 3.59 pmol/l (n = 8) and a maximum number of binding sites (Bmax) of 1.6 ± 0.15 fmol/mg protein (n = 8). The Kd value determined from kinetic analysis was 16.5 ± 3.02 pmol/l (n = 3). Competition studies using various indoles and neurotransmitters demonstrated that 2-iodomelatonin, melatonin, 6-chloromelatonin, 6- hydroxymelatonin and N-acetylserotonin showed significant inhibition of the 2-[125I]iodomelatonin binding, while the other indole compounds tested had no significant inhibition. The expression of rat uterine endometrial melatonin receptor subtypes was studied by reverse transcription-polymerase chain reaction (RT-PCR) using mt1 and MT2 receptor gene-specific primers. mt1 receptor cDNA was amplified and confirmed by nucleotide sequencing. These findings indicate that mt1 receptors were present in the rat uterine endometrium, and suggest that melatonin plays an integral part in uterine physiology.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_HK
dc.relation.ispartofLife Sciencesen_HK
dc.rightsLife Sciences. Copyright © Elsevier Inc.en_HK
dc.subject2-iodomelatoninen_HK
dc.subjectFemale reproductive systemen_HK
dc.subjectN- acetylserotoninen_HK
dc.subjectPineal glanden_HK
dc.titlePharmacological characterization, molecular subtyping, and autoradiographic localization of putative melatonin receptors in uterine endometrium of estrous ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0024-3205&volume=66 &issue=17&spage=1581&epage=1591&date=2000&atitle=Pharmacological+characterization,+molecular+subtyping,+and+autoradiographic+localization+of+putative+melatonin+receptors+in+uterine+endometrium+of+estrous+ratsen_HK
dc.identifier.emailPoon, AMS: amspoon@hkucc.hku.hken_HK
dc.identifier.authorityPoon, AMS=rp00354en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0024-3205(00)00478-1en_HK
dc.identifier.pmid11261588-
dc.identifier.scopuseid_2-s2.0-0034678094en_HK
dc.identifier.hkuros53272en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034678094&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume66en_HK
dc.identifier.issue17en_HK
dc.identifier.spage1581en_HK
dc.identifier.epage1591en_HK
dc.identifier.isiWOS:000086593600003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhao, H=14821083400en_HK
dc.identifier.scopusauthoridPoon, AMS=7103068868en_HK
dc.identifier.scopusauthoridPang, SF=7402528719en_HK

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