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Article: Melatonin and 2[125I]iodomelatonin binding sites in the human colon

TitleMelatonin and 2[125I]iodomelatonin binding sites in the human colon
Authors
Issue Date1996
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/07435800.asp
Citation
Endocrine Research, 1996, v. 22 n. 1, p. 77-94 How to Cite?
Abstract2[125I]Iodomelatonin binding sites were identified in the mucosa of the human colon from Chinese patients with carcinoma of the rectum or colon using biochemical receptor assay and autoradiography. Melatonin in the colonic mucosa/submucosa and muscle layers were quantitated by radioimmunoassay. The binding of 2[125I]iodomelatonin to the membrane preparations of the human colonic mucosa/submucosa was stable, saturable, reversible and of high affinity. Rosenthal analysis from saturation studies performed at 21°C yielded an equilibrium dissociation constant (Kd) of 61.7±4.48 pmol/L (n=3) and maximum number of binding sites (Bmax) of 1.65±0.51 fmol/mg protein (n=3). The linearity of the Rosenthal plots and unity of the Hill coefficient suggested that 2[125I]iodomelatonin was bound to a single class of binding sites. The radioligand binding was displaced by 2-iodomelatonin (Ki=0.02 nmol/L), melatonin (0.65 nmol/L), 6-chloromelatonin (Ki=5.33 nmol/L), 6-hydroxymelatonin (Ki=33.8 nmol/L) and N-acetylserotonin (Ki= 122 nmol/L). The characteristics of the binding sites were similar to those reported in the jejunum of duck, chicken and human but of higher affinity than those in the mouse colon. Autoradiography localizes the binding to the mucosa of the human colon. Radioimmunoassay revealed a melatonin concentration of 467±99 pg/g wet tissue of human colon (n=6). Our findings suggest that melatonin may influence the human colonie functions through interaction with its receptors in the mucosa.
Persistent Identifierhttp://hdl.handle.net/10722/81141
ISSN
2015 Impact Factor: 1.476
2015 SCImago Journal Rankings: 0.539
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPoon, AMSen_HK
dc.contributor.authorMak, ASYen_HK
dc.contributor.authorLuk, HTen_HK
dc.date.accessioned2010-09-06T08:14:18Z-
dc.date.available2010-09-06T08:14:18Z-
dc.date.issued1996en_HK
dc.identifier.citationEndocrine Research, 1996, v. 22 n. 1, p. 77-94en_HK
dc.identifier.issn0743-5800en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81141-
dc.description.abstract2[125I]Iodomelatonin binding sites were identified in the mucosa of the human colon from Chinese patients with carcinoma of the rectum or colon using biochemical receptor assay and autoradiography. Melatonin in the colonic mucosa/submucosa and muscle layers were quantitated by radioimmunoassay. The binding of 2[125I]iodomelatonin to the membrane preparations of the human colonic mucosa/submucosa was stable, saturable, reversible and of high affinity. Rosenthal analysis from saturation studies performed at 21°C yielded an equilibrium dissociation constant (Kd) of 61.7±4.48 pmol/L (n=3) and maximum number of binding sites (Bmax) of 1.65±0.51 fmol/mg protein (n=3). The linearity of the Rosenthal plots and unity of the Hill coefficient suggested that 2[125I]iodomelatonin was bound to a single class of binding sites. The radioligand binding was displaced by 2-iodomelatonin (Ki=0.02 nmol/L), melatonin (0.65 nmol/L), 6-chloromelatonin (Ki=5.33 nmol/L), 6-hydroxymelatonin (Ki=33.8 nmol/L) and N-acetylserotonin (Ki= 122 nmol/L). The characteristics of the binding sites were similar to those reported in the jejunum of duck, chicken and human but of higher affinity than those in the mouse colon. Autoradiography localizes the binding to the mucosa of the human colon. Radioimmunoassay revealed a melatonin concentration of 467±99 pg/g wet tissue of human colon (n=6). Our findings suggest that melatonin may influence the human colonie functions through interaction with its receptors in the mucosa.en_HK
dc.languageengen_HK
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/07435800.aspen_HK
dc.relation.ispartofEndocrine Researchen_HK
dc.rightsEndocrine Research. Copyright © Informa Healthcare.en_HK
dc.titleMelatonin and 2[125I]iodomelatonin binding sites in the human colonen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0743-5800&volume=22&issue=1&spage=77&epage=94&date=1996&atitle=Melatonin+and+2[125I]iodomelatonin+binding+sites+in+the+human+colonen_HK
dc.identifier.emailPoon, AMS: amspoon@hkucc.hku.hken_HK
dc.identifier.authorityPoon, AMS=rp00354en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8690008-
dc.identifier.scopuseid_2-s2.0-0029670379en_HK
dc.identifier.hkuros15380en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029670379&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue1en_HK
dc.identifier.spage77en_HK
dc.identifier.epage94en_HK
dc.identifier.isiWOS:A1996UD11700005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridPoon, AMS=7103068868en_HK
dc.identifier.scopusauthoridMak, ASY=35945385300en_HK
dc.identifier.scopusauthoridLuk, HT=7006636051en_HK

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