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Article: Puerarin, an isoflavonoid derived from Radix puerariae, potentiates endothelium-independent relaxation via the cyclic AMP pathway in porcine coronary artery

TitlePuerarin, an isoflavonoid derived from Radix puerariae, potentiates endothelium-independent relaxation via the cyclic AMP pathway in porcine coronary artery
Authors
Keywords3′-5′-cyclic adenosine monophosphate (cyclic AMP)
Puerarin
Radix puerariae
Vascular relaxation
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
Citation
European Journal Of Pharmacology, 2006, v. 552 n. 1-3, p. 105-111 How to Cite?
AbstractPuerarin, an isoflavonoid derived from the Chinese medicinal herb Radix puerariae, has been suggested to be useful in the management of various cardiovascular disorders. The present study examined the effect of acute exposure (30 min) to puerarin on vascular relaxation. Rings from porcine coronary artery of either sex were used. The highest concentration of puerarin (100 μM) produced a small but statistically significant relaxation of U46619-contracted rings. Vascular relaxations were also studied in the presence of lower concentrations of puerarin (0.1, 1 and 10 μM) which had no direct relaxation effect. Puerarin enhanced vasorelaxation to endothelium-independent relaxing agents, sodium nitroprusside and cromakalim. However, puerarin had no effect on vasorelaxation induced by endothelium-dependent relaxing agents, bradykinin and calcium ionophore A23187. The potentiating action of puerarin (10 μM) on sodium nitroprusside-mediated relaxation was not affected by the nitric oxide synthase inhibitor, Nω-nitro-l-arginine methyl ester (l-NAME; 300 μM), or by the disruption of the endothelium with Triton X-100. The effect of puerarin was reversible following a washout period. The potentiating effects were comparable with the 3′-5′-cyclic adenosine monophosphate (cyclic AMP) analogues, 8-bromoadenosine-3′-5′-cyclic monophosphate (8-Br-cyclic AMP; 10 μM) and Sp-isomer [S nomenclature refers to phosphorus] of adenosine-3′, 5′-cyclic monophosphorothioate (Sp-cyclic AMPS; 3 μM), but not the 3′-5′-cyclic guanosine monophosphate (cyclic GMP) analogue, 8-bromoguanosine-3′-5′-cyclic monophosphate (8-Br-cyclic GMP; 3 μM). The cyclic AMP antagonist, Rp-isomer [R nomenclature refers to phosphorus] of 8-bromoadenosine-3′, 5′-cyclic monophosphorothioate (Rp-8-Br-cyclic AMPS; 10 μM), but not cyclic GMP antagonist, Rp-isomer of 8-bromoguanosine-3′, 5′-cyclic monophosphorothioate (Rp-8-Br-cyclic GMPS; 10 μM), reversed the effects of puerarin (10 μM) on the enhancement of vasorelaxation to sodium nitroprusside. Our results demonstrated that puerarin enhanced sodium nitroprusside-induced relaxation, possibly via the cyclic AMP-dependent pathway. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/80336
ISSN
2015 Impact Factor: 2.73
2015 SCImago Journal Rankings: 1.115
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, DKYen_HK
dc.contributor.authorLeung, SWSen_HK
dc.contributor.authorXu, YCen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.contributor.authorMan, RYKen_HK
dc.date.accessioned2010-09-06T08:05:11Z-
dc.date.available2010-09-06T08:05:11Z-
dc.date.issued2006en_HK
dc.identifier.citationEuropean Journal Of Pharmacology, 2006, v. 552 n. 1-3, p. 105-111en_HK
dc.identifier.issn0014-2999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80336-
dc.description.abstractPuerarin, an isoflavonoid derived from the Chinese medicinal herb Radix puerariae, has been suggested to be useful in the management of various cardiovascular disorders. The present study examined the effect of acute exposure (30 min) to puerarin on vascular relaxation. Rings from porcine coronary artery of either sex were used. The highest concentration of puerarin (100 μM) produced a small but statistically significant relaxation of U46619-contracted rings. Vascular relaxations were also studied in the presence of lower concentrations of puerarin (0.1, 1 and 10 μM) which had no direct relaxation effect. Puerarin enhanced vasorelaxation to endothelium-independent relaxing agents, sodium nitroprusside and cromakalim. However, puerarin had no effect on vasorelaxation induced by endothelium-dependent relaxing agents, bradykinin and calcium ionophore A23187. The potentiating action of puerarin (10 μM) on sodium nitroprusside-mediated relaxation was not affected by the nitric oxide synthase inhibitor, Nω-nitro-l-arginine methyl ester (l-NAME; 300 μM), or by the disruption of the endothelium with Triton X-100. The effect of puerarin was reversible following a washout period. The potentiating effects were comparable with the 3′-5′-cyclic adenosine monophosphate (cyclic AMP) analogues, 8-bromoadenosine-3′-5′-cyclic monophosphate (8-Br-cyclic AMP; 10 μM) and Sp-isomer [S nomenclature refers to phosphorus] of adenosine-3′, 5′-cyclic monophosphorothioate (Sp-cyclic AMPS; 3 μM), but not the 3′-5′-cyclic guanosine monophosphate (cyclic GMP) analogue, 8-bromoguanosine-3′-5′-cyclic monophosphate (8-Br-cyclic GMP; 3 μM). The cyclic AMP antagonist, Rp-isomer [R nomenclature refers to phosphorus] of 8-bromoadenosine-3′, 5′-cyclic monophosphorothioate (Rp-8-Br-cyclic AMPS; 10 μM), but not cyclic GMP antagonist, Rp-isomer of 8-bromoguanosine-3′, 5′-cyclic monophosphorothioate (Rp-8-Br-cyclic GMPS; 10 μM), reversed the effects of puerarin (10 μM) on the enhancement of vasorelaxation to sodium nitroprusside. Our results demonstrated that puerarin enhanced sodium nitroprusside-induced relaxation, possibly via the cyclic AMP-dependent pathway. © 2006 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejpharen_HK
dc.relation.ispartofEuropean Journal of Pharmacologyen_HK
dc.rightsEuropean Journal of Pharmacology. Copyright © Elsevier BV.en_HK
dc.subject3′-5′-cyclic adenosine monophosphate (cyclic AMP)en_HK
dc.subjectPuerarinen_HK
dc.subjectRadix puerariaeen_HK
dc.subjectVascular relaxationen_HK
dc.titlePuerarin, an isoflavonoid derived from Radix puerariae, potentiates endothelium-independent relaxation via the cyclic AMP pathway in porcine coronary arteryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-2999&volume=287&spage=101&epage=105&date=2006&atitle=Puerarin,+an+isoflavonoid+derived+from+Radix+puerariae,+potentiates+endothelium-independent+relaxation+via+the+cyclic+AMP+pathway+in+porcine+coronary+artery.en_HK
dc.identifier.emailLeung, SWS: swsleung@hku.hken_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.authorityLeung, SWS=rp00235en_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ejphar.2006.08.078en_HK
dc.identifier.pmid17027964-
dc.identifier.scopuseid_2-s2.0-33750480231en_HK
dc.identifier.hkuros130663en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33750480231&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume552en_HK
dc.identifier.issue1-3en_HK
dc.identifier.spage105en_HK
dc.identifier.epage111en_HK
dc.identifier.isiWOS:000242452500014-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridYeung, DKY=16176892200en_HK
dc.identifier.scopusauthoridLeung, SWS=24540419500en_HK
dc.identifier.scopusauthoridXu, YC=14039899700en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK

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