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Article: Characterization of adenosine transport in H9c2 cardiomyoblasts

TitleCharacterization of adenosine transport in H9c2 cardiomyoblasts
Authors
KeywordsAdenosine
Heart
Nucleoside transporters
Issue Date2007
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ijcard
Citation
International Journal Of Cardiology, 2007, v. 116 n. 2, p. 186-193 How to Cite?
AbstractAdenosine plays a significant role in various physiological processes including cardioprotection. Nucleoside transporters modulate adenosine levels in the vicinity of adenosine receptors, which in turn modulate adenosine functional efficacy. In the current study, adenosine transport in the rat heart myoblast cell line H9c2 was characterized. Kinetic analysis of adenosine transport in H9c2 cells revealed a Km of 8.9 ± 0.001 μM and a Vmax of 32.1 ± 0.65 pmol/mg protein/min. Adenosine transport in H9c2 cells was Na+-independent. About 6% of the total adenosine uptake was sensitive to nitrobenzylmercaptopurine riboside (NBMPR); however, 94% was insensitive, suggesting that adenosine uptake by H9c2 cells was predominantly mediated by the equilibrative nucleoside transporter (ENT)-2 and only mildly by ENT-1. Results of RT-PCR demonstrated the presence of mRNA for ENT-1, ENT-2 and ENT-3. Upon culture in a cell differentiation medium containing fetal bovine serum (1%) and retinoic acid (10 nM), both the activity and mRNA expression of ENT-1 increased 3-fold, however, ENT-2 was unaffected. Pharmacological studies revealed that ENT-1 activity was stimulated by PKA and PKC-δ/ε, however, ENT-2 activity was unaffected. Taken together, the exceptionally high expression level of ENT-2 in H9c2 cells raises questions regarding the use of H9c2 cells as a model for physiological adenosine activity in the heart. Furthermore, this study may form the basis for further investigation into the effect of cell differentiation and protein kinases on the regulation of nucleoside transporters. © 2006 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/80328
ISSN
2015 Impact Factor: 4.638
2015 SCImago Journal Rankings: 1.513
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorTse, CMen_HK
dc.contributor.authorMan, RYKen_HK
dc.date.accessioned2010-09-06T08:05:05Z-
dc.date.available2010-09-06T08:05:05Z-
dc.date.issued2007en_HK
dc.identifier.citationInternational Journal Of Cardiology, 2007, v. 116 n. 2, p. 186-193en_HK
dc.identifier.issn0167-5273en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80328-
dc.description.abstractAdenosine plays a significant role in various physiological processes including cardioprotection. Nucleoside transporters modulate adenosine levels in the vicinity of adenosine receptors, which in turn modulate adenosine functional efficacy. In the current study, adenosine transport in the rat heart myoblast cell line H9c2 was characterized. Kinetic analysis of adenosine transport in H9c2 cells revealed a Km of 8.9 ± 0.001 μM and a Vmax of 32.1 ± 0.65 pmol/mg protein/min. Adenosine transport in H9c2 cells was Na+-independent. About 6% of the total adenosine uptake was sensitive to nitrobenzylmercaptopurine riboside (NBMPR); however, 94% was insensitive, suggesting that adenosine uptake by H9c2 cells was predominantly mediated by the equilibrative nucleoside transporter (ENT)-2 and only mildly by ENT-1. Results of RT-PCR demonstrated the presence of mRNA for ENT-1, ENT-2 and ENT-3. Upon culture in a cell differentiation medium containing fetal bovine serum (1%) and retinoic acid (10 nM), both the activity and mRNA expression of ENT-1 increased 3-fold, however, ENT-2 was unaffected. Pharmacological studies revealed that ENT-1 activity was stimulated by PKA and PKC-δ/ε, however, ENT-2 activity was unaffected. Taken together, the exceptionally high expression level of ENT-2 in H9c2 cells raises questions regarding the use of H9c2 cells as a model for physiological adenosine activity in the heart. Furthermore, this study may form the basis for further investigation into the effect of cell differentiation and protein kinases on the regulation of nucleoside transporters. © 2006 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ijcarden_HK
dc.relation.ispartofInternational Journal of Cardiologyen_HK
dc.rightsInternational Journal of Cardiology. Copyright © Elsevier Ireland Ltd.en_HK
dc.subjectAdenosineen_HK
dc.subjectHearten_HK
dc.subjectNucleoside transportersen_HK
dc.titleCharacterization of adenosine transport in H9c2 cardiomyoblastsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-5273&volume=116&spage=186&epage=193&date=2006&atitle=Characterization+of+adenosine+transport+in+H9c2+cardiomyoblastsen_HK
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ijcard.2006.03.044en_HK
dc.identifier.pmid16824629-
dc.identifier.scopuseid_2-s2.0-33846919304en_HK
dc.identifier.hkuros136453en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846919304&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume116en_HK
dc.identifier.issue2en_HK
dc.identifier.spage186en_HK
dc.identifier.epage193en_HK
dc.identifier.eissn1874-1754-
dc.identifier.isiWOS:000245087700008-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridLeung, GPH=35963668200en_HK
dc.identifier.scopusauthoridTse, CM=7103295076en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK

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